Methods description - Notes 13C-labeling experiment - for metabolic flux analysis, 13C-labeling experiments were initiated after taking samples for transcriptome, proteome and metabolome analysis. For experiments with gene disruptants, the feed medium containing 4 g/l of natural glucose was replaced by
...
an identical medium containing 0.4 g/l of [1-13C] glucose, 0.4 g/l of uniformly labeled [U-13C] glucose and 3.2 g/l of natural glucose. For experiments examining the effect of changes in dilution rate on flux distributions, the composition of glucose in the feed medium was changed to 0.8 g/l of [1-13C] glucose, 0.8 g/l of [U-13C] glucose and 2.4 g/l of natural glucose. After two residence times, for gas chromatography-mass spectrometry (GC-MS) analysis, E. coli cells were harvested by centrifugation.
--------------------References---------------
http://doi.org/cdjhrn
http://doi.org/cr7qpd
Read more
Data file
Preview file
Information
galM
glk
pgm
pgi
pfkA_2
pfkB
fbp
fbaB
gapC
gpmA
gpmB
pykA
pykF
ppsA
zwf
pgl
gnd
rpe
rpiA
rpiB
tktA
tktB
talA
talB
WT_0.1
WT_0.4
WT_0.5
WT_0.7
Metadata
Data EntryID
35
Entered by
Administrator KiMoSys
Date Added
2013-04-22
Version
3
General Information
Manuscript title
Multiple High-Throughput Analyses Monitor the Response of E. coli to Perturbations.
PubMed ID
17379776
Journal
Science
Year
2007
Authors
Nobuyoshi Ishii, Kenji Nakahigashi, Tomoya Baba, Martin Robert, Tomoyoshi Soga, Akio Kanai, Takashi Hirasawa, Miki Naba, Kenta Hirai, Aminul Hoque, Pei Yee Ho, Yuji Kakazu, Kaori Sugawara, Saori Igarashi, Satoshi Harada, Takeshi Masuda, Naoyuki Sugiyama, Takashi Togashi, Miki Hasegawa, Yuki Takai, Katsuyuki Yugi, Kazuharu Arakawa, Nayuta Iwata, Yoshihiro Toya, Yoichi Nakayama, Takaaki Nishioka, Kazuyuki Shimizu, Hirotada Mori, Masaru Tomita
Affiliations
Institute for Advanced Biosciences, Keio University
Keywords
Escherichia coli, perturbations response, chemostat, single knockouts
Project name
not specified
Experiment Description
Organism
Escherichia coli
Strain
WT K-12 BW25113 and mutants
Data type
flux measurements
Data units
(mmol/g-dry cell weight/h)
Execution date
not specified
Experimental Details
Temperature (0C)
37
pH
7.0
Carbon source
glucose
Culture mode
chemostat
Process condition
aerobic
Dilution rate (h-1)
0.1, 0.2, 0.4, 0.5 and 0.7
Working volume (L)
1.0
Biomass concentration (g/L)
See Supplementary files of the original article (Tabel S3).
Medium composition
Synthetic medium: 48mM Na2HPO4, 22mM KH2PO4, 10mM NaCl, 45mM (NH4)2SO4, 4g/L glucose, 1mM MgSO4, 1mg/L thiamin.HCL, 5.6 mg/L CaCl2, 8 mg/L FeCl3, 1 mg/L MnCl2.4H2O, 1.7 mg/L ZnCl2, 0.43 mg/L CuCl2.2H2O, 0.6 mg/L CoCl2.2H2O and 0.6 mg/L Na2MoO4.2H2O
General protocol information
Flux analysis method: 13C constrained MFA
Platform: GC-MS
Methods description - Notes
13C-labeling experiment - for metabolic flux analysis, 13C-labeling experiments were initiated after taking samples for transcriptome, proteome and metabolome analysis. For experiments with gene disruptants, the feed medium containing 4 g/l of natural glucose was replaced by an identical medium containing 0.4 g/l of [1-13C] glucose, 0.4 g/l of uniformly labeled [U-13C] glucose and 3.2 g/l of natural glucose. For experiments examining the effect of changes in dilution rate on flux distributions, the composition of glucose in the feed medium was changed to 0.8 g/l of [1-13C] glucose, 0.8 g/l of [U-13C] glucose and 2.4 g/l of natural glucose. After two residence times, for gas chromatography-mass spectrometry (GC-MS) analysis, E. coli cells were harvested by centrifugation.
GC-MS analysis - the cells obtained from about 250 ml of culture were suspended in 4 ml of 6 M HCl and then hydrolyzed at 105 ºC for 16 h. After cooling, HCl was evaporated with a centrifugal evaporator (CVE-3100, Tokyo Rikakikai Co., Ltd., Japan). The dried hydrolysate was resuspended in water and then filtrated through a 0.22-μm pore size filter (Millipore Co., USA). The filtrate was dried again and redissolved in 1.5 ml of acetonitrile. For derivatization, the resulting 80 μl of biomass hydrolysate dissolved in acetonitrile was mixed with an equal volume of N-methyl-N-(tert-butyldimethylsilyl)-trifluoroacetamide and then incubated at 110 oC for 30 min. After cooling, the derivatized sample was used for the GC-MS analysis using a TurboMass Gold mass spectrometer (Perkin Elmer, USA). In the present study, two fragment ions, [M-57]+ and [M-159]+, of tert-butyldimethylsilylated (TBDMS-) amino acids (Ala, Gly, Val, Ile, Pro, Ser, Met, Phe, Asp, Glu and Tyr) were monitored. The analytical conditions for GC-MS were as described by Zhao et al [1].
Estimation of metabolic flux distribution - for metabolic flux analysis, we constructed a basic stoichiometric reaction model for the main metabolic pathways including glycolysis, the pentose phosphate pathway, the tricarboxylic acid (TCA) cycle, the glyoxylate shunt
and the anaplerotic pathway. The biomass content reported by Li et al. [2] was used for calculations.
--------------------References---------------
[1] J. Zhao, K. Shimizu, J. Biotechnol. 101, 101 (2003).
[2] M. Li, P. Y. Ho, S. Yao, K. Shimizu, J. Biotechnol. 122, 254 (2006).
Data URL
http://kdbio.inesc-id.pt/kimosys/repository/35
Reaction
KEGG ID
Flux
Unit
Glucose + PEP -> G6P + PYR
R02738 3.04
mmol/g*h
G6P F6P
R02740 1.95
mmol/g*h
F6P -> F1.6P
R04779 2.46
mmol/g*h
F1.6P -> DHAP + G3P
R01070 2.46
mmol/g*h
DHAP -> G3P
R01015 2.46
mmol/g*h
G3P -> 3PG
R01061 5.12
mmol/g*h
3PG PEP
4.82
mmol/g*h
PEP -> PYR
R00200 1.68
mmol/g*h
PYR -> AcCoA + CO2
R00209 4.27
mmol/g*h
G6P -> 6PG
1.05
mmol/g*h
6PG -> Ru5P + CO2
R01528 1.05
mmol/g*h
Ru5P -> X5P
R01529 0.54
mmol/g*h
Ru5P -> R5P
R01056 0.51
mmol/g*h
R5P + X5P S7P + G3P
R01641 0.31
mmol/g*h
S7P + G3P E4P + F6P
R01827 0.31
mmol/g*h
X5P + E4P F6P + G3P
R01830 0.23
mmol/g*h
AcCoA + OAA -> CIT
R00351 2.65
mmol/g*h
CIT -> ICT
R01324 2.65
mmol/g*h
ICT -> 2-KG + CO2
R00267 1.85
mmol/g*h
2-KG -> SUC + CO2
R08549 1.61
mmol/g*h
SUC -> FUM
R00412 2.41
mmol/g*h
FUM -> MAL
R01082 2.41
mmol/g*h
MAL OAA
R00342 3.05
mmol/g*h
PEP + CO2 OAA
R00341 0.0
mmol/g*h
MAL -> PYR + CO2
R00214 0.17
mmol/g*h
ICT -> Glyoxylate + SUC
R00479 0.8
mmol/g*h
Glyoxylate + AcCoA -> MAL
R00472 0.8
mmol/g*h
6-PG -> G3P + PYR
R01064 0.0
mmol/g*h
AcCoA -> Acetate
R00227 0.0
mmol/g*h
PYR -> Lactate
R00704 0.0
mmol/g*h
AcCoA -> Ethanol
0.0
mmol/g*h
G6P -> (Cell synthesis)
0.04
mmol/g*h
F6P -> (Cell synthesis)
0.02
mmol/g*h
R5P -> (Cell synthesis)
0.2
mmol/g*h
E4P -> (Cell synthesis)
0.09
mmol/g*h
G3P -> (Cell synthesis)
0.03
mmol/g*h
3PG -> (Cell synthesis)
0.3
mmol/g*h
PEP -> (Cell synthesis)
0.11
mmol/g*h
PYR -> (Cell synthesis)
0.61
mmol/g*h
AcCoA -> (Cell synthesis)
0.82
mmol/g*h
OAA -> (Cell synthesis)
0.4
mmol/g*h
2KG -> (Cell synthesis)
0.24
mmol/g*h
CO2 -> (Evolution)
8.95
mmol/g*h
Exch. (G6P F6P)
0.03
mmol/g*h
Exch. (3PG PEP)
0.0
mmol/g*h
Exch. (R5P + X5P S7P + G3P)
0.0
mmol/g*h
Exch. (S7P + G3P E4P + F6P)
0.02
mmol/g*h
Exch. (X5P + E4P F6P + G3P)
0.0
mmol/g*h
Exch. (MAL OAA)
0.03
mmol/g*h
Exch. (PEP + CO2 OAA)
0.01
mmol/g*h
Glucose consumption rate
3.038194104929218
mmol/g*h
Oxygen uptake rate (OUR)
15.530458590006845
mmol/g*h
Carbon dioxide evolution rate (CER)
5.763175906913073
mmol/g*h
Ethanol production rate
0.0
mmol/g*h
Acetate production rate
0.0008885332082302536
mmol/g*h
D-Lactate production rate
0.0
mmol/g*h
Succinate production rate
0.0031128484072383473
mmol/g*h
Pyruvate production rate
0.0008591191377242199
mmol/g*h
Formate production rate
0.0
mmol/g*h
Graph Loading...
Reaction
KEGG ID
Flux
Unit
Glucose + PEP -> G6P + PYR
R02738 3.14
mmol/g*h
G6P F6P
R02740 2.72
mmol/g*h
F6P -> F1,6P
R04779 2.79
mmol/g*h
F1,6P -> DHAP + G3P
R01070 2.79
mmol/g*h
DHAP -> G3P
R01015 2.79
mmol/g*h
G3P -> 3PG
R01061 5.55
mmol/g*h
3PG PEP
5.25
mmol/g*h
PEP -> PYR
R00200 1.6
mmol/g*h
PYR -> AcCoA + CO2
R00209 4.13
mmol/g*h
G6P -> 6PG
0.37
mmol/g*h
6PG -> Ru5P + CO2
R01528 0.37
mmol/g*h
Ru5P -> X5P
R01529 0.09
mmol/g*h
Ru5P -> R5P
R01056 0.28
mmol/g*h
R5P + X5P S7P + G3P
R01641 0.09
mmol/g*h
S7P + G3P E4P + F6P
R01827 0.09
mmol/g*h
X5P + E4P F6P + G3P
R01830 0
mmol/g*h
AcCoA + OAA -> CIT
R00351 3.09
mmol/g*h
CIT -> ICT
R01324 3.09
mmol/g*h
ICT -> 2-KG + CO2
R00267 2.86
mmol/g*h
2-KG -> SUC + CO2
R08549 2.61
mmol/g*h
SUC -> FUM
R00412 2.84
mmol/g*h
FUM -> MAL
R01082 2.84
mmol/g*h
MAL OAA
R00342 3.07
mmol/g*h
PEP + CO2 OAA
R00341 0.41
mmol/g*h
MAL -> PYR + CO2
R00214 0
mmol/g*h
ICT -> Glyoxylate + SUC
R00479 0.23
mmol/g*h
Glyoxylate + AcCoA -> MAL
R00472 0.23
mmol/g*h
6-PG -> G3P + PYR
R01064 mmol/g*h
AcCoA -> Acetate
R00227 0
mmol/g*h
PYR -> Lactate
R00704 0
mmol/g*h
AcCoA -> Ethanol
0
mmol/g*h
G6P -> (Cell synthesis)
0.04
mmol/g*h
F6P -> (Cell synthesis)
0.02
mmol/g*h
R5P -> (Cell synthesis)
0.19
mmol/g*h
E4P -> (Cell synthesis)
0.09
mmol/g*h
G3P -> (Cell synthesis)
0.03
mmol/g*h
3PG -> (Cell synthesis)
0.3
mmol/g*h
PEP -> (Cell synthesis)
0.11
mmol/g*h
PYR -> (Cell synthesis)
0.61
mmol/g*h
AcCoA -> (Cell synthesis)
0.82
mmol/g*h
OAA -> (Cell synthesis)
0.4
mmol/g*h
2KG -> (Cell synthesis)
0.24
mmol/g*h
CO2 -> (Evolution)
9.57
mmol/g*h
Exch. (G6P F6P)
0.03
mmol/g*h
Exch. (3PG PEP)
0
mmol/g*h
Exch. (R5P + X5P S7P + G3P)
0
mmol/g*h
Exch. (S7P + G3P E4P + F6P)
0.02
mmol/g*h
Exch. (X5P + E4P F6P + G3P)
0
mmol/g*h
Exch. (MAL OAA)
0.03
mmol/g*h
Exch. (PEP + CO2 OAA)
0.01
mmol/g*h
Glucose consumption rate
3.1434234902648504
mmol/g*h
Oxygen uptake rate (OUR)
5.4390934844192635
mmol/g*h
Carbon dioxide evolution rate (CER)
6.458923512747875
mmol/g*h
Ethanol production rate
0.0
mmol/g*h
Acetate production rate
0.0005254138688484833
mmol/g*h
D-Lactate production rate
3.021718602455149e-05
mmol/g*h
Succinate production rate
0.0008281962885656499
mmol/g*h
Pyruvate production rate
0.0007017890227831436
mmol/g*h
Formate production rate
0.0027566437339808436
mmol/g*h
Graph Loading...
Reaction
KEGG ID
Flux
Unit
Glucose + PEP -> G6P + PYR
R02738 3.06
mmol/g*h
G6P F6P
R02740 2.39
mmol/g*h
F6P -> F1,6P
R04779 2.63
mmol/g*h
F1,6P -> DHAP + G3P
R01070 2.63
mmol/g*h
DHAP -> G3P
R01015 2.63
mmol/g*h
G3P -> 3PG
R01061 5.3
mmol/g*h
3PG PEP
5.01
mmol/g*h
PEP -> PYR
R00200 1.54
mmol/g*h
PYR -> AcCoA + CO2
R00209 3.99
mmol/g*h
G6P -> 6PG
0.63
mmol/g*h
6PG -> Ru5P + CO2
R01528 0.63
mmol/g*h
Ru5P -> X5P
R01529 0.26
mmol/g*h
Ru5P -> R5P
R01056 0.37
mmol/g*h
R5P + X5P S7P + G3P
R01641 0.17
mmol/g*h
S7P + G3P E4P + F6P
R01827 0.17
mmol/g*h
X5P + E4P F6P + G3P
R01830 0.09
mmol/g*h
AcCoA + OAA -> CIT
R00351 2.83
mmol/g*h
CIT -> ICT
R01324 2.83
mmol/g*h
ICT -> 2-KG + CO2
R00267 2.5
mmol/g*h
2-KG -> SUC + CO2
R08549 2.26
mmol/g*h
SUC -> FUM
R00412 2.59
mmol/g*h
FUM -> MAL
R01082 2.59
mmol/g*h
MAL OAA
R00342 2.93
mmol/g*h
PEP + CO2 OAA
R00341 0.3
mmol/g*h
MAL -> PYR + CO2
R00214 0
mmol/g*h
ICT -> Glyoxylate + SUC
R00479 0.34
mmol/g*h
Glyoxylate + AcCoA -> MAL
R00472 0.34
mmol/g*h
6-PG -> G3P + PYR
R01064 0
mmol/g*h
AcCoA -> Acetate
R00227 0
mmol/g*h
PYR -> Lactate
R00704 0
mmol/g*h
AcCoA -> Ethanol
0
mmol/g*h
G6P -> (Cell synthesis)
0.04
mmol/g*h
F6P -> (Cell synthesis)
0.02
mmol/g*h
R5P -> (Cell synthesis)
0.2
mmol/g*h
E4P -> (Cell synthesis)
0.09
mmol/g*h
G3P -> (Cell synthesis)
0.03
mmol/g*h
3PG -> (Cell synthesis)
0.3
mmol/g*h
PEP -> (Cell synthesis)
0.11
mmol/g*h
PYR -> (Cell synthesis)
0.61
mmol/g*h
AcCoA -> (Cell synthesis)
0.82
mmol/g*h
OAA -> (Cell synthesis)
0.39
mmol/g*h
2KG -> (Cell synthesis)
0.24
mmol/g*h
CO2 -> (Evolution)
9.07
mmol/g*h
mmol/g*h
Exch. (G6P F6P)
0.03
mmol/g*h
Exch. (3PG PEP)
0.03
mmol/g*h
Exch. (R5P + X5P S7P + G3P)
0
mmol/g*h
Exch. (S7P + G3P E4P + F6P)
0.02
mmol/g*h
Exch. (X5P + E4P F6P + G3P)
0
mmol/g*h
Exch. (MAL OAA)
0.03
mmol/g*h
Exch. (PEP + CO2 OAA)
0.011
mmol/g*h
Glucose consumption rate
3.0587230700228627
mmol/g*h
Oxygen uptake rate (OUR)
5.789110957960028
mmol/g*h
Carbon dioxide evolution rate (CER)
6.119917298414887
mmol/g*h
Ethanol production rate
0.0
mmol/g*h
Acetate production rate
0.0008125982081323225
mmol/g*h
D-Lactate production rate
1.960027567195038e-05
mmol/g*h
Succinate production rate
0.004029662301860785
mmol/g*h
Pyruvate production rate
0.00037563059958649206
mmol/g*h
Formate production rate
0.0
mmol/g*h
Graph Loading...
Reaction
KEGG ID
Flux
Unit
Glucose + PEP -> G6P + PYR
R02738 2.73
mmol/g*h
G6P F6P
R02740 0
mmol/g*h
F6P -> F1,6P
R04779 0.93
mmol/g*h
F1,6P -> DHAP + G3P
R01070 0.93
mmol/g*h
DHAP -> G3P
R01015 0.93
mmol/g*h
G3P -> 3PG
R01061 3.28
mmol/g*h
3PG PEP
2.98
mmol/g*h
PEP -> PYR
R00200 0.14
mmol/g*h
PYR -> AcCoA + CO2
R00209 3.34
mmol/g*h
G6P -> 6PG
2.69
mmol/g*h
6PG -> Ru5P + CO2
R01528 1.67
mmol/g*h
Ru5P -> X5P
R01529 0.95
mmol/g*h
Ru5P -> R5P
R01056 0.72
mmol/g*h
R5P + X5P S7P + G3P
R01641 0.52
mmol/g*h
S7P + G3P E4P + F6P
R01827 0.52
mmol/g*h
X5P + E4P F6P + G3P
R01830 0.43
mmol/g*h
AcCoA + OAA -> CIT
R00351 1.82
mmol/g*h
CIT -> ICT
R01324 1.82
mmol/g*h
ICT -> 2-KG + CO2
R00267 1.13
mmol/g*h
2-KG -> SUC + CO2
R08549 0.89
mmol/g*h
SUC -> FUM
R00412 1.58
mmol/g*h
FUM -> MAL
R01082 1.58
mmol/g*h
MAL OAA
R00342 2.22
mmol/g*h
PEP + CO2 OAA
R00341 0
mmol/g*h
MAL -> PYR + CO2
R00214 0.06
mmol/g*h
ICT -> Glyoxylate + SUC
R00479 0.69
mmol/g*h
Glyoxylate + AcCoA -> MAL
R00472 0.69
mmol/g*h
6-PG -> G3P + PYR
R01064 1.02
mmol/g*h
AcCoA -> Acetate
R00227 0
mmol/g*h
PYR -> Lactate
R00704 0
mmol/g*h
AcCoA -> Ethanol
0
mmol/g*h
G6P -> (Cell synthesis)
0.04
mmol/g*h
F6P -> (Cell synthesis)
0.02
mmol/g*h
R5P -> (Cell synthesis)
0.2
mmol/g*h
E4P -> (Cell synthesis)
0.09
mmol/g*h
G3P -> (Cell synthesis)
0.03
mmol/g*h
3PG -> (Cell synthesis)
0.3
mmol/g*h
PEP -> (Cell synthesis)
0.11
mmol/g*h
PYR -> (Cell synthesis)
0.61
mmol/g*h
AcCoA -> (Cell synthesis)
0.82
mmol/g*h
OAA -> (Cell synthesis)
0.4
mmol/g*h
2KG -> (Cell synthesis)
0.24
mmol/g*h
CO2 -> (Evolution)
7.08
mmol/g*h
Exch. (G6P F6P)
mmol/g*h
Exch. (3PG PEP)
0.01
mmol/g*h
Exch. (R5P + X5P S7P + G3P)
0.03
mmol/g*h
Exch. (S7P + G3P E4P + F6P)
0.01
mmol/g*h
Exch. (X5P + E4P F6P + G3P)
0
mmol/g*h
Exch. (MAL OAA)
0.01
mmol/g*h
Exch. (PEP + CO2 OAA)
0.002
mmol/g*h
Glucose consumption rate
2.726693608635548
mmol/g*h
Oxygen uptake rate (OUR)
4.4471896232242125
mmol/g*h
Carbon dioxide evolution rate (CER)
5.0401482396541075
mmol/g*h
Ethanol production rate
0.0029036566934357365
mmol/g*h
Acetate production rate
0.0005155376285306863
mmol/g*h
D-Lactate production rate
0.0
mmol/g*h
Succinate production rate
0.0009029107804285025
mmol/g*h
Pyruvate production rate
0.00036111574990746416
mmol/g*h
Formate production rate
0.0020752571154053533
mmol/g*h
Graph Loading...
Reaction
KEGG ID
Flux
Unit
Glucose + PEP -> G6P + PYR
R02738 2.72
mmol/g*h
G6P F6P
R02740 2.3
mmol/g*h
F6P -> F1,6P
R04779 2.37
mmol/g*h
F1,6P -> DHAP + G3P
R01070 2.37
mmol/g*h
DHAP -> G3P
R01015 2.37
mmol/g*h
G3P -> 3PG
R01061 4.7
mmol/g*h
3PG PEP
4.4
mmol/g*h
PEP -> PYR
R00200 1.16
mmol/g*h
PYR -> AcCoA + CO2
R00209 3.26
mmol/g*h
G6P -> 6PG
0.38
mmol/g*h
6PG -> Ru5P + CO2
R01528 0.38
mmol/g*h
Ru5P -> X5P
R01529 0.09
mmol/g*h
Ru5P -> R5P
R01056 0.29
mmol/g*h
R5P + X5P S7P + G3P
R01641 0.09
mmol/g*h
S7P + G3P E4P + F6P
R01827 0.09
mmol/g*h
X5P + E4P F6P + G3P
R01830 0
mmol/g*h
AcCoA + OAA -> CIT
R00351 2.22
mmol/g*h
CIT -> ICT
R01324 2.22
mmol/g*h
ICT -> 2-KG + CO2
R00267 2
mmol/g*h
2-KG -> SUC + CO2
R08549 1.75
mmol/g*h
SUC -> FUM
R00412 1.97
mmol/g*h
FUM -> MAL
R01082 1.97
mmol/g*h
MAL OAA
R00342 2.2
mmol/g*h
PEP + CO2 OAA
R00341 0.42
mmol/g*h
MAL -> PYR + CO2
R00214 0
mmol/g*h
ICT -> Glyoxylate + SUC
R00479 0.22
mmol/g*h
Glyoxylate + AcCoA -> MAL
R00472 0.22
mmol/g*h
6-PG -> G3P + PYR
R01064 mmol/g*h
AcCoA -> Acetate
R00227 0
mmol/g*h
PYR -> Lactate
R00704 0
mmol/g*h
AcCoA -> Ethanol
0
mmol/g*h
G6P -> (Cell synthesis)
0.04
mmol/g*h
F6P -> (Cell synthesis)
0.02
mmol/g*h
R5P -> (Cell synthesis)
0.2
mmol/g*h
E4P -> (Cell synthesis)
0.09
mmol/g*h
G3P -> (Cell synthesis)
0.03
mmol/g*h
3PG -> (Cell synthesis)
0.3
mmol/g*h
PEP -> (Cell synthesis)
0.11
mmol/g*h
PYR -> (Cell synthesis)
0.61
mmol/g*h
AcCoA -> (Cell synthesis)
0.82
mmol/g*h
OAA -> (Cell synthesis)
0.4
mmol/g*h
2KG -> (Cell synthesis)
0.24
mmol/g*h
CO2 -> (Evolution)
6.97
mmol/g*h
Exch. (G6P F6P)
0.03
mmol/g*h
Exch. (3PG PEP)
0.03
mmol/g*h
Exch. (R5P + X5P S7P + G3P)
0
mmol/g*h
Exch. (S7P + G3P E4P + F6P)
0.01
mmol/g*h
Exch. (X5P + E4P F6P + G3P)
0
mmol/g*h
Exch. (MAL OAA)
0.03
mmol/g*h
Exch. (PEP + CO2 OAA)
0.0121312
mmol/g*h
Glucose consumption rate
2.71858500622471
mmol/g*h
Oxygen uptake rate (OUR)
9.558823529411764
mmol/g*h
Carbon dioxide evolution rate (CER)
2.9411764705882355
mmol/g*h
Ethanol production rate
0.0044740101380252575
mmol/g*h
Acetate production rate
0.0
mmol/g*h
D-Lactate production rate
0.0
mmol/g*h
Succinate production rate
0.00095541985812303
mmol/g*h
Pyruvate production rate
0.0005221791949424184
mmol/g*h
Formate production rate
0.0001423902894491131
mmol/g*h
Graph Loading...
Reaction
KEGG ID
Flux
Unit
Glucose + PEP -> G6P + PYR
R02738 2.84
mmol/g*h
G6P F6P
R02740 1.47
mmol/g*h
F6P -> F1,6P
R04779 2.18
mmol/g*h
F1,6P -> DHAP + G3P
R01070 2.18
mmol/g*h
DHAP -> G3P
R01015 2.18
mmol/g*h
G3P -> 3PG
R01061 4.64
mmol/g*h
3PG PEP
4.34
mmol/g*h
PEP -> PYR
R00200 1.38
mmol/g*h
PYR -> AcCoA + CO2
R00209 3.69
mmol/g*h
G6P -> 6PG
1.32
mmol/g*h
6PG -> Ru5P + CO2
R01528 1.32
mmol/g*h
Ru5P -> X5P
R01529 0.72
mmol/g*h
Ru5P -> R5P
R01056 0.6
mmol/g*h
R5P + X5P S7P + G3P
R01641 0.41
mmol/g*h
S7P + G3P E4P + F6P
R01827 0.41
mmol/g*h
X5P + E4P F6P + G3P
R01830 0.32
mmol/g*h
AcCoA + OAA -> CIT
R00351 2.16
mmol/g*h
CIT -> ICT
R01324 2.16
mmol/g*h
ICT -> 2-KG + CO2
R00267 1.46
mmol/g*h
2-KG -> SUC + CO2
R08549 1.21
mmol/g*h
SUC -> FUM
R00412 1.92
mmol/g*h
FUM -> MAL
R01082 1.92
mmol/g*h
MAL OAA
R00342 2.55
mmol/g*h
PEP + CO2 OAA
R00341 0.01
mmol/g*h
MAL -> PYR + CO2
R00214 0.08
mmol/g*h
ICT -> Glyoxylate + SUC
R00479 0.71
mmol/g*h
Glyoxylate + AcCoA -> MAL
R00472 0.71
mmol/g*h
6-PG -> G3P + PYR
R01064 mmol/g*h
AcCoA -> Acetate
R00227 0
mmol/g*h
PYR -> Lactate
R00704 0
mmol/g*h
AcCoA -> Ethanol
0
mmol/g*h
G6P -> (Cell synthesis)
0.04
mmol/g*h
F6P -> (Cell synthesis)
0.02
mmol/g*h
R5P -> (Cell synthesis)
0.2
mmol/g*h
E4P -> (Cell synthesis)
0.09
mmol/g*h
G3P -> (Cell synthesis)
0.03
mmol/g*h
3PG -> (Cell synthesis)
0.3
mmol/g*h
PEP -> (Cell synthesis)
0.11
mmol/g*h
PYR -> (Cell synthesis)
0.61
mmol/g*h
AcCoA -> (Cell synthesis)
0.82
mmol/g*h
OAA -> (Cell synthesis)
0.39
mmol/g*h
2KG -> (Cell synthesis)
0.24
mmol/g*h
CO2 -> (Evolution)
7.76
mmol/g*h
Exch. (G6P F6P)
0.02
mmol/g*h
Exch. (3PG PEP)
0
mmol/g*h
Exch. (R5P + X5P S7P + G3P)
0
mmol/g*h
Exch. (S7P + G3P E4P + F6P)
0.02
mmol/g*h
Exch. (X5P + E4P F6P + G3P)
0
mmol/g*h
Exch. (MAL OAA)
0.02
mmol/g*h
Exch. (PEP + CO2 OAA)
0.0078668
mmol/g*h
Glucose consumption rate
2.8375506028498356
mmol/g*h
Oxygen uptake rate (OUR)
5.831202046035805
mmol/g*h
Carbon dioxide evolution rate (CER)
3.836317135549872
mmol/g*h
Ethanol production rate
0.0
mmol/g*h
Acetate production rate
0.0006831752462645339
mmol/g*h
D-Lactate production rate
0.0
mmol/g*h
Succinate production rate
0.010405583846383584
mmol/g*h
Pyruvate production rate
0.0002470979405794262
mmol/g*h
Formate production rate
0.0
mmol/g*h
Graph Loading...
Reaction
KEGG ID
Flux
Unit
Glucose + PEP -> G6P + PYR
R02738 2.7
mmol/g*h
G6P F6P
R02740 2.29
mmol/g*h
F6P -> F1,6P
R04779 2.35
mmol/g*h
F1,6P -> DHAP + G3P
R01070 2.35
mmol/g*h
DHAP -> G3P
R01015 2.35
mmol/g*h
G3P -> 3PG
R01061 4.67
mmol/g*h
3PG PEP
4.37
mmol/g*h
PEP -> PYR
R00200 1.54
mmol/g*h
PYR -> AcCoA + CO2
R00209 3.84
mmol/g*h
G6P -> 6PG
0.37
mmol/g*h
6PG -> Ru5P + CO2
R01528 0.37
mmol/g*h
Ru5P -> X5P
R01529 0.09
mmol/g*h
Ru5P -> R5P
R01056 0.28
mmol/g*h
R5P + X5P S7P + G3P
R01641 0.09
mmol/g*h
S7P + G3P E4P + F6P
R01827 0.09
mmol/g*h
X5P + E4P F6P + G3P
R01830 0
mmol/g*h
AcCoA + OAA -> CIT
R00351 2.21
mmol/g*h
CIT -> ICT
R01324 2.21
mmol/g*h
ICT -> 2-KG + CO2
R00267 1.39
mmol/g*h
2-KG -> SUC + CO2
R08549 1.15
mmol/g*h
SUC -> FUM
R00412 1.97
mmol/g*h
FUM -> MAL
R01082 1.97
mmol/g*h
MAL OAA
R00342 2.57
mmol/g*h
PEP + CO2 OAA
R00341 0.03
mmol/g*h
MAL -> PYR + CO2
R00214 0.21
mmol/g*h
ICT -> Glyoxylate + SUC
R00479 0.82
mmol/g*h
Glyoxylate + AcCoA -> MAL
R00472 0.82
mmol/g*h
6-PG -> G3P + PYR
R01064 mmol/g*h
AcCoA -> Acetate
R00227 0
mmol/g*h
PYR -> Lactate
R00704 0
mmol/g*h
AcCoA -> Ethanol
0
mmol/g*h
G6P -> (Cell synthesis)
0.04
mmol/g*h
F6P -> (Cell synthesis)
0.02
mmol/g*h
R5P -> (Cell synthesis)
0.2
mmol/g*h
E4P -> (Cell synthesis)
0.09
mmol/g*h
G3P -> (Cell synthesis)
0.03
mmol/g*h
3PG -> (Cell synthesis)
0.3
mmol/g*h
PEP -> (Cell synthesis)
0.11
mmol/g*h
PYR -> (Cell synthesis)
0.61
mmol/g*h
AcCoA -> (Cell synthesis)
0.82
mmol/g*h
OAA -> (Cell synthesis)
0.39
mmol/g*h
2KG -> (Cell synthesis)
0.24
mmol/g*h
CO2 -> (Evolution)
6.93
mmol/g*h
mmol/g*h
Exch. (G6P F6P)
0.02
mmol/g*h
Exch. (3PG PEP)
0.03
mmol/g*h
Exch. (R5P + X5P S7P + G3P)
0
mmol/g*h
Exch. (S7P + G3P E4P + F6P)
0.02
mmol/g*h
Exch. (X5P + E4P F6P + G3P)
0
mmol/g*h
Exch. (MAL OAA)
0.02
mmol/g*h
Exch. (PEP + CO2 OAA)
0.010881
mmol/g*h
Glucose consumption rate
2.704785080719267
mmol/g*h
Oxygen uptake rate (OUR)
5.996343692870201
mmol/g*h
Carbon dioxide evolution rate (CER)
4.095063985374772
mmol/g*h
Ethanol production rate
0.0
mmol/g*h
Acetate production rate
0.0004260235916640919
mmol/g*h
D-Lactate production rate
6.066761459814482e-05
mmol/g*h
Succinate production rate
0.0
mmol/g*h
Pyruvate production rate
0.0004951611225711337
mmol/g*h
Formate production rate
0.0
mmol/g*h
Graph Loading...
Reaction
KEGG ID
Flux
Unit
Glucose + PEP -> G6P + PYR
R02738 2.76
mmol/g*h
G6P F6P
R02740 2.02
mmol/g*h
F6P -> F1,6P
R04779 2.3
mmol/g*h
F1,6P -> DHAP + G3P
R01070 2.3
mmol/g*h
DHAP -> G3P
R01015 2.3
mmol/g*h
G3P -> 3PG
R01061 4.68
mmol/g*h
3PG PEP
4.38
mmol/g*h
PEP -> PYR
R00200 1.24
mmol/g*h
PYR -> AcCoA + CO2
R00209 3.39
mmol/g*h
G6P -> 6PG
0.7
mmol/g*h
6PG -> Ru5P + CO2
R01528 0.7
mmol/g*h
Ru5P -> X5P
R01529 0.3
mmol/g*h
Ru5P -> R5P
R01056 0.39
mmol/g*h
R5P + X5P S7P + G3P
R01641 0.2
mmol/g*h
S7P + G3P E4P + F6P
R01827 0.2
mmol/g*h
X5P + E4P F6P + G3P
R01830 0.11
mmol/g*h
AcCoA + OAA -> CIT
R00351 2.21
mmol/g*h
CIT -> ICT
R01324 2.21
mmol/g*h
ICT -> 2-KG + CO2
R00267 1.85
mmol/g*h
2-KG -> SUC + CO2
R08549 1.61
mmol/g*h
SUC -> FUM
R00412 1.97
mmol/g*h
FUM -> MAL
R01082 1.97
mmol/g*h
MAL OAA
R00342 2.33
mmol/g*h
PEP + CO2 OAA
R00341 0.27
mmol/g*h
MAL -> PYR + CO2
R00214 0
mmol/g*h
ICT -> Glyoxylate + SUC
R00479 0.36
mmol/g*h
Glyoxylate + AcCoA -> MAL
R00472 0.36
mmol/g*h
6-PG -> G3P + PYR
R01064 mmol/g*h
AcCoA -> Acetate
R00227 0
mmol/g*h
PYR -> Lactate
R00704 0
mmol/g*h
AcCoA -> Ethanol
0
mmol/g*h
G6P -> (Cell synthesis)
0.04
mmol/g*h
F6P -> (Cell synthesis)
0.02
mmol/g*h
R5P -> (Cell synthesis)
0.2
mmol/g*h
E4P -> (Cell synthesis)
0.09
mmol/g*h
G3P -> (Cell synthesis)
0.03
mmol/g*h
3PG -> (Cell synthesis)
0.3
mmol/g*h
PEP -> (Cell synthesis)
0.11
mmol/g*h
PYR -> (Cell synthesis)
0.61
mmol/g*h
AcCoA -> (Cell synthesis)
0.82
mmol/g*h
OAA -> (Cell synthesis)
0.39
mmol/g*h
2KG -> (Cell synthesis)
0.24
mmol/g*h
CO2 -> (Evolution)
7.27
mmol/g*h
Exch. (G6P F6P)
0.03
mmol/g*h
Exch. (3PG PEP)
0
mmol/g*h
Exch. (R5P + X5P S7P + G3P)
0
mmol/g*h
Exch. (S7P + G3P E4P + F6P)
0.02
mmol/g*h
Exch. (X5P + E4P F6P + G3P)
0
mmol/g*h
Exch. (MAL OAA)
0.03
mmol/g*h
Exch. (PEP + CO2 OAA)
0.0079764
mmol/g*h
Glucose consumption rate
2.7588358527372527
mmol/g*h
Oxygen uptake rate (OUR)
8.084840923268871
mmol/g*h
Carbon dioxide evolution rate (CER)
2.395508421709295
mmol/g*h
Ethanol production rate
0.00018718971821474867
mmol/g*h
Acetate production rate
0.0005795123522937471
mmol/g*h
D-Lactate production rate
0.00011533929437859332
mmol/g*h
Succinate production rate
0.0
mmol/g*h
Pyruvate production rate
0.00036472014915794556
mmol/g*h
Formate production rate
0.0
mmol/g*h
Graph Loading...
Reaction
KEGG ID
Flux
Unit
Glucose + PEP -> G6P + PYR
R02738 2.99
mmol/g*h
G6P F6P
R02740 1.87
mmol/g*h
F6P -> F1,6P
R04779 2.41
mmol/g*h
F1,6P -> DHAP + G3P
R01070 2.41
mmol/g*h
DHAP -> G3P
R01015 2.41
mmol/g*h
G3P -> 3PG
R01061 5.01
mmol/g*h
3PG PEP
4.72
mmol/g*h
PEP -> PYR
R00200 1.62
mmol/g*h
PYR -> AcCoA + CO2
R00209 4.3
mmol/g*h
G6P -> 6PG
1.08
mmol/g*h
6PG -> Ru5P + CO2
R01528 1.08
mmol/g*h
Ru5P -> X5P
R01529 0.56
mmol/g*h
Ru5P -> R5P
R01056 0.52
mmol/g*h
R5P + X5P S7P + G3P
R01641 0.32
mmol/g*h
S7P + G3P E4P + F6P
R01827 0.32
mmol/g*h
X5P + E4P F6P + G3P
R01830 0.23
mmol/g*h
AcCoA + OAA -> CIT
R00351 2.54
mmol/g*h
CIT -> ICT
R01324 2.54
mmol/g*h
ICT -> 2-KG + CO2
R00267 1.61
mmol/g*h
2-KG -> SUC + CO2
R08549 1.36
mmol/g*h
SUC -> FUM
R00412 2.3
mmol/g*h
FUM -> MAL
R01082 2.3
mmol/g*h
MAL OAA
R00342 2.94
mmol/g*h
PEP + CO2 OAA
R00341 0
mmol/g*h
MAL -> PYR + CO2
R00214 0.3
mmol/g*h
ICT -> Glyoxylate + SUC
R00479 0.94
mmol/g*h
Glyoxylate + AcCoA -> MAL
R00472 0.94
mmol/g*h
6-PG -> G3P + PYR
R01064 mmol/g*h
AcCoA -> Acetate
R00227 0
mmol/g*h
PYR -> Lactate
R00704 0
mmol/g*h
AcCoA -> Ethanol
0
mmol/g*h
G6P -> (Cell synthesis)
0.04
mmol/g*h
F6P -> (Cell synthesis)
0.02
mmol/g*h
R5P -> (Cell synthesis)
0.2
mmol/g*h
E4P -> (Cell synthesis)
0.09
mmol/g*h
G3P -> (Cell synthesis)
0.03
mmol/g*h
3PG -> (Cell synthesis)
0.3
mmol/g*h
PEP -> (Cell synthesis)
0.11
mmol/g*h
PYR -> (Cell synthesis)
0.61
mmol/g*h
AcCoA -> (Cell synthesis)
0.82
mmol/g*h
OAA -> (Cell synthesis)
0.39
mmol/g*h
2KG -> (Cell synthesis)
0.24
mmol/g*h
CO2 -> (Evolution)
8.64
mmol/g*h
mmol/g*h
Exch. (G6P F6P)
0.03
mmol/g*h
Exch. (3PG PEP)
0
mmol/g*h
Exch. (R5P + X5P S7P + G3P)
0
mmol/g*h
Exch. (S7P + G3P E4P + F6P)
0.02
mmol/g*h
Exch. (X5P + E4P F6P + G3P)
0
mmol/g*h
Exch. (MAL OAA)
0.03
mmol/g*h
Exch. (PEP + CO2 OAA)
0.0074152
mmol/g*h
Glucose consumption rate
2.99027957679086
mmol/g*h
Oxygen uptake rate (OUR)
6.790165038733581
mmol/g*h
Carbon dioxide evolution rate (CER)
6.143482654092287
mmol/g*h
Ethanol production rate
0.002425585328109738
mmol/g*h
Acetate production rate
0.0004968135794682484
mmol/g*h
D-Lactate production rate
6.706335840724371e-05
mmol/g*h
Succinate production rate
0.00032822868515063443
mmol/g*h
Pyruvate production rate
0.0008877950240659002
mmol/g*h
Formate production rate
0.0070507412575449005
mmol/g*h
Graph Loading...
Reaction
KEGG ID
Flux
Unit
Glucose + PEP -> G6P + PYR
R02738 2.7
mmol/g*h
G6P F6P
R02740 2.23
mmol/g*h
F6P -> F1,6P
R04779 2.33
mmol/g*h
F1,6P -> DHAP + G3P
R01070 2.33
mmol/g*h
DHAP -> G3P
R01015 2.33
mmol/g*h
G3P -> 3PG
R01061 4.65
mmol/g*h
3PG PEP
4.35
mmol/g*h
PEP -> PYR
R00200 1.1
mmol/g*h
PYR -> AcCoA + CO2
R00209 3.19
mmol/g*h
G6P -> 6PG
0.42
mmol/g*h
6PG -> Ru5P + CO2
R01528 0.42
mmol/g*h
Ru5P -> X5P
R01529 0.12
mmol/g*h
Ru5P -> R5P
R01056 0.3
mmol/g*h
R5P + X5P S7P + G3P
R01641 0.11
mmol/g*h
S7P + G3P E4P + F6P
R01827 0.11
mmol/g*h
X5P + E4P F6P + G3P
R01830 0.02
mmol/g*h
AcCoA + OAA -> CIT
R00351 2.17
mmol/g*h
CIT -> ICT
R01324 2.17
mmol/g*h
ICT -> 2-KG + CO2
R00267 1.98
mmol/g*h
2-KG -> SUC + CO2
R08549 1.74
mmol/g*h
SUC -> FUM
R00412 1.93
mmol/g*h
FUM -> MAL
R01082 1.93
mmol/g*h
MAL OAA
R00342 2.13
mmol/g*h
PEP + CO2 OAA
R00341 0.45
mmol/g*h
MAL -> PYR + CO2
R00214 0
mmol/g*h
ICT -> Glyoxylate + SUC
R00479 0.19
mmol/g*h
Glyoxylate + AcCoA -> MAL
R00472 0.19
mmol/g*h
6-PG -> G3P + PYR
R01064 mmol/g*h
AcCoA -> Acetate
R00227 0
mmol/g*h
PYR -> Lactate
R00704 0
mmol/g*h
AcCoA -> Ethanol
0
mmol/g*h
G6P -> (Cell synthesis)
0.04
mmol/g*h
F6P -> (Cell synthesis)
0.02
mmol/g*h
R5P -> (Cell synthesis)
0.2
mmol/g*h
E4P -> (Cell synthesis)
0.09
mmol/g*h
G3P -> (Cell synthesis)
0.03
mmol/g*h
3PG -> (Cell synthesis)
0.3
mmol/g*h
PEP -> (Cell synthesis)
0.11
mmol/g*h
PYR -> (Cell synthesis)
0.61
mmol/g*h
AcCoA -> (Cell synthesis)
0.82
mmol/g*h
OAA -> (Cell synthesis)
0.4
mmol/g*h
2KG -> (Cell synthesis)
0.24
mmol/g*h
CO2 -> (Evolution)
6.89
mmol/g*h
mmol/g*h
Exch. (G6P F6P)
0.03
mmol/g*h
Exch. (3PG PEP)
0.03
mmol/g*h
Exch. (R5P + X5P S7P + G3P)
0
mmol/g*h
Exch. (S7P + G3P E4P + F6P)
0.02
mmol/g*h
Exch. (X5P + E4P F6P + G3P)
0
mmol/g*h
Exch. (MAL OAA)
0.02
mmol/g*h
Exch. (PEP + CO2 OAA)
0.008289
mmol/g*h
Glucose consumption rate
2.696029461242944
mmol/g*h
Oxygen uptake rate (OUR)
6.705132098390525
mmol/g*h
Carbon dioxide evolution rate (CER)
4.810203461888855
mmol/g*h
Ethanol production rate
0.0020046864872642887
mmol/g*h
Acetate production rate
0.0
mmol/g*h
D-Lactate production rate
0.0
mmol/g*h
Succinate production rate
0.0019532042115846036
mmol/g*h
Pyruvate production rate
0.0004935070769142001
mmol/g*h
Formate production rate
0.0
mmol/g*h
Graph Loading...
Reaction
KEGG ID
Flux
Unit
Glucose + PEP -> G6P + PYR
R02738 3.33
mmol/g*h
G6P F6P
R02740 2.92
mmol/g*h
F6P -> F1,6P
R04779 2.98
mmol/g*h
F1,6P -> DHAP + G3P
R01070 2.98
mmol/g*h
DHAP -> G3P
R01015 2.98
mmol/g*h
G3P -> 3PG
R01061 5.93
mmol/g*h
3PG PEP
5.64
mmol/g*h
PEP -> PYR
R00200 1.83
mmol/g*h
PYR -> AcCoA + CO2
R00209 4.56
mmol/g*h
G6P -> 6PG
0.37
mmol/g*h
6PG -> Ru5P + CO2
R01528 0.37
mmol/g*h
Ru5P -> X5P
R01529 0.09
mmol/g*h
Ru5P -> R5P
R01056 0.28
mmol/g*h
R5P + X5P S7P + G3P
R01641 0.09
mmol/g*h
S7P + G3P E4P + F6P
R01827 0.09
mmol/g*h
X5P + E4P F6P + G3P
R01830 0
mmol/g*h
AcCoA + OAA -> CIT
R00351 3.47
mmol/g*h
CIT -> ICT
R01324 3.47
mmol/g*h
ICT -> 2-KG + CO2
R00267 3.2
mmol/g*h
2-KG -> SUC + CO2
R08549 2.96
mmol/g*h
SUC -> FUM
R00412 3.23
mmol/g*h
FUM -> MAL
R01082 3.23
mmol/g*h
MAL OAA
R00342 3.5
mmol/g*h
PEP + CO2 OAA
R00341 0.37
mmol/g*h
MAL -> PYR + CO2
R00214 0
mmol/g*h
ICT -> Glyoxylate + SUC
R00479 0.27
mmol/g*h
Glyoxylate + AcCoA -> MAL
R00472 0.27
mmol/g*h
6-PG -> G3P + PYR
R01064 mmol/g*h
AcCoA -> Acetate
R00227 0
mmol/g*h
PYR -> Lactate
R00704 0
mmol/g*h
AcCoA -> Ethanol
0
mmol/g*h
G6P -> (Cell synthesis)
0.04
mmol/g*h
F6P -> (Cell synthesis)
0.02
mmol/g*h
R5P -> (Cell synthesis)
0.2
mmol/g*h
E4P -> (Cell synthesis)
0.09
mmol/g*h
G3P -> (Cell synthesis)
0.03
mmol/g*h
3PG -> (Cell synthesis)
0.3
mmol/g*h
PEP -> (Cell synthesis)
0.11
mmol/g*h
PYR -> (Cell synthesis)
0.61
mmol/g*h
AcCoA -> (Cell synthesis)
0.82
mmol/g*h
OAA -> (Cell synthesis)
0.4
mmol/g*h
2KG -> (Cell synthesis)
0.24
mmol/g*h
CO2 -> (Evolution)
10.72
mmol/g*h
mmol/g*h
Exch. (G6P F6P)
0.03
mmol/g*h
Exch. (3PG PEP)
0.03
mmol/g*h
Exch. (R5P + X5P S7P + G3P)
0
mmol/g*h
Exch. (S7P + G3P E4P + F6P)
0.02
mmol/g*h
Exch. (X5P + E4P F6P + G3P)
0
mmol/g*h
Exch. (MAL OAA)
0.03
mmol/g*h
Exch. (PEP + CO2 OAA)
0.0154512
mmol/g*h
Glucose consumption rate
3.3314979264979265
mmol/g*h
Oxygen uptake rate (OUR)
5.045045045045044
mmol/g*h
Carbon dioxide evolution rate (CER)
5.045045045045044
mmol/g*h
Ethanol production rate
0.0
mmol/g*h
Acetate production rate
0.0
mmol/g*h
D-Lactate production rate
0.0
mmol/g*h
Succinate production rate
0.0
mmol/g*h
Pyruvate production rate
0.0003099743806837445
mmol/g*h
Formate production rate
0.0
mmol/g*h
Graph Loading...
Reaction
KEGG ID
Flux
Unit
Glucose + PEP -> G6P + PYR
R02738 2.82
mmol/g*h
G6P F6P
R02740 2.1
mmol/g*h
F6P -> F1,6P
R04779 2.37
mmol/g*h
F1,6P -> DHAP + G3P
R01070 2.37
mmol/g*h
DHAP -> G3P
R01015 2.37
mmol/g*h
G3P -> 3PG
R01061 4.8
mmol/g*h
3PG PEP
4.5
mmol/g*h
PEP -> PYR
R00200 1.44
mmol/g*h
PYR -> AcCoA + CO2
R00209 3.65
mmol/g*h
G6P -> 6PG
0.67
mmol/g*h
6PG -> Ru5P + CO2
R01528 0.67
mmol/g*h
Ru5P -> X5P
R01529 0.29
mmol/g*h
Ru5P -> R5P
R01056 0.39
mmol/g*h
R5P + X5P S7P + G3P
R01641 0.19
mmol/g*h
S7P + G3P E4P + F6P
R01827 0.19
mmol/g*h
X5P + E4P F6P + G3P
R01830 0.1
mmol/g*h
AcCoA + OAA -> CIT
R00351 2.33
mmol/g*h
CIT -> ICT
R01324 2.33
mmol/g*h
ICT -> 2-KG + CO2
R00267 1.84
mmol/g*h
2-KG -> SUC + CO2
R08549 1.6
mmol/g*h
SUC -> FUM
R00412 2.09
mmol/g*h
FUM -> MAL
R01082 2.09
mmol/g*h
MAL OAA
R00342 2.59
mmol/g*h
PEP + CO2 OAA
R00341 0.14
mmol/g*h
MAL -> PYR + CO2
R00214 0
mmol/g*h
ICT -> Glyoxylate + SUC
R00479 0.5
mmol/g*h
Glyoxylate + AcCoA -> MAL
R00472 0.5
mmol/g*h
6-PG -> G3P + PYR
R01064 mmol/g*h
AcCoA -> Acetate
R00227 0
mmol/g*h
PYR -> Lactate
R00704 0
mmol/g*h
AcCoA -> Ethanol
0
mmol/g*h
G6P -> (Cell synthesis)
0.05
mmol/g*h
F6P -> (Cell synthesis)
0.02
mmol/g*h
R5P -> (Cell synthesis)
0.2
mmol/g*h
E4P -> (Cell synthesis)
0.09
mmol/g*h
G3P -> (Cell synthesis)
0.03
mmol/g*h
3PG -> (Cell synthesis)
0.3
mmol/g*h
PEP -> (Cell synthesis)
0.11
mmol/g*h
PYR -> (Cell synthesis)
0.61
mmol/g*h
AcCoA -> (Cell synthesis)
0.82
mmol/g*h
OAA -> (Cell synthesis)
0.39
mmol/g*h
2KG -> (Cell synthesis)
0.24
mmol/g*h
CO2 -> (Evolution)
7.62
mmol/g*h
Exch. (G6P F6P)
0.03
mmol/g*h
Exch. (3PG PEP)
0.02
mmol/g*h
Exch. (R5P + X5P S7P + G3P)
0
mmol/g*h
Exch. (S7P + G3P E4P + F6P)
0.02
mmol/g*h
Exch. (X5P + E4P F6P + G3P)
0
mmol/g*h
Exch. (MAL OAA)
0.03
mmol/g*h
Exch. (PEP + CO2 OAA)
0.0082344
mmol/g*h
Glucose consumption rate
2.8212117983027074
mmol/g*h
Oxygen uptake rate (OUR)
7.171010807374444
mmol/g*h
Carbon dioxide evolution rate (CER)
5.034965034965035
mmol/g*h
Ethanol production rate
0.0
mmol/g*h
Acetate production rate
0.0011371433083820306
mmol/g*h
D-Lactate production rate
0.0
mmol/g*h
Succinate production rate
0.0018586300743977336
mmol/g*h
Pyruvate production rate
0.00043047224101463907
mmol/g*h
Formate production rate
0.0010566874203237868
mmol/g*h
Graph Loading...
Reaction
KEGG ID
Flux
Unit
Glucose + PEP -> G6P + PYR
R02738 2.8
mmol/g*h
G6P F6P
R02740 1.3
mmol/g*h
F6P -> F1,6P
R04779 2.09
mmol/g*h
F1,6P -> DHAP + G3P
R01070 2.09
mmol/g*h
DHAP -> G3P
R01015 2.09
mmol/g*h
G3P -> 3PG
R01061 4.51
mmol/g*h
3PG PEP
4.21
mmol/g*h
PEP -> PYR
R00200 1.3
mmol/g*h
PYR -> AcCoA + CO2
R00209 3.69
mmol/g*h
G6P -> 6PG
1.45
mmol/g*h
6PG -> Ru5P + CO2
R01528 1.45
mmol/g*h
Ru5P -> X5P
R01529 0.81
mmol/g*h
Ru5P -> R5P
R01056 0.64
mmol/g*h
R5P + X5P S7P + G3P
R01641 0.45
mmol/g*h
S7P + G3P E4P + F6P
R01827 0.45
mmol/g*h
X5P + E4P F6P + G3P
R01830 0.36
mmol/g*h
AcCoA + OAA -> CIT
R00351 2.04
mmol/g*h
CIT -> ICT
R01324 2.04
mmol/g*h
ICT -> 2-KG + CO2
R00267 1.2
mmol/g*h
2-KG -> SUC + CO2
R08549 0.96
mmol/g*h
SUC -> FUM
R00412 1.8
mmol/g*h
FUM -> MAL
R01082 1.8
mmol/g*h
MAL OAA
R00342 2.43
mmol/g*h
PEP + CO2 OAA
R00341 0.01
mmol/g*h
MAL -> PYR + CO2
R00214 0.21
mmol/g*h
ICT -> Glyoxylate + SUC
R00479 0.83
mmol/g*h
Glyoxylate + AcCoA -> MAL
R00472 0.83
mmol/g*h
6-PG -> G3P + PYR
R01064 mmol/g*h
AcCoA -> Acetate
R00227 0
mmol/g*h
PYR -> Lactate
R00704 0
mmol/g*h
AcCoA -> Ethanol
0
mmol/g*h
G6P -> (Cell synthesis)
0.04
mmol/g*h
F6P -> (Cell synthesis)
0.02
mmol/g*h
R5P -> (Cell synthesis)
0.2
mmol/g*h
E4P -> (Cell synthesis)
0.09
mmol/g*h
G3P -> (Cell synthesis)
0.03
mmol/g*h
3PG -> (Cell synthesis)
0.3
mmol/g*h
PEP -> (Cell synthesis)
0.11
mmol/g*h
PYR -> (Cell synthesis)
0.61
mmol/g*h
AcCoA -> (Cell synthesis)
0.82
mmol/g*h
OAA -> (Cell synthesis)
0.39
mmol/g*h
2KG -> (Cell synthesis)
0.24
mmol/g*h
CO2 -> (Evolution)
7.51
mmol/g*h
Exch. (G6P F6P)
0.02
mmol/g*h
Exch. (3PG PEP)
0
mmol/g*h
Exch. (R5P + X5P S7P + G3P)
0
mmol/g*h
Exch. (S7P + G3P E4P + F6P)
0.02
mmol/g*h
Exch. (X5P + E4P F6P + G3P)
0
mmol/g*h
Exch. (MAL OAA)
0.03
mmol/g*h
Exch. (PEP + CO2 OAA)
0.007952
mmol/g*h
Glucose consumption rate
2.8048010107391033
mmol/g*h
Oxygen uptake rate (OUR)
6.0644346178142765
mmol/g*h
Carbon dioxide evolution rate (CER)
4.851547694251421
mmol/g*h
Ethanol production rate
0.0
mmol/g*h
Acetate production rate
0.0006255337965887556
mmol/g*h
D-Lactate production rate
0.0
mmol/g*h
Succinate production rate
0.000543790271636134
mmol/g*h
Pyruvate production rate
0.000166923562855338
mmol/g*h
Formate production rate
3.566645609602022e-05
mmol/g*h
Graph Loading...
Reaction
KEGG ID
Flux
Unit
Glucose + PEP -> G6P + PYR
R02738 3
mmol/g*h
G6P F6P
R02740 2.03
mmol/g*h
F6P -> F1,6P
R04779 2.47
mmol/g*h
F1,6P -> DHAP + G3P
R01070 2.47
mmol/g*h
DHAP -> G3P
R01015 2.47
mmol/g*h
G3P -> 3PG
R01061 5.09
mmol/g*h
3PG PEP
4.79
mmol/g*h
PEP -> PYR
R00200 1.04
mmol/g*h
PYR -> AcCoA + CO2
R00209 3.43
mmol/g*h
G6P -> 6PG
0.93
mmol/g*h
6PG -> Ru5P + CO2
R01528 0.93
mmol/g*h
Ru5P -> X5P
R01529 0.46
mmol/g*h
Ru5P -> R5P
R01056 0.47
mmol/g*h
R5P + X5P S7P + G3P
R01641 0.27
mmol/g*h
S7P + G3P E4P + F6P
R01827 0.27
mmol/g*h
X5P + E4P F6P + G3P
R01830 0.19
mmol/g*h
AcCoA + OAA -> CIT
R00351 2.61
mmol/g*h
CIT -> ICT
R01324 2.61
mmol/g*h
ICT -> 2-KG + CO2
R00267 2.61
mmol/g*h
2-KG -> SUC + CO2
R08549 2.37
mmol/g*h
SUC -> FUM
R00412 2.37
mmol/g*h
FUM -> MAL
R01082 2.37
mmol/g*h
MAL OAA
R00342 2.37
mmol/g*h
PEP + CO2 OAA
R00341 0.64
mmol/g*h
MAL -> PYR + CO2
R00214 0
mmol/g*h
ICT -> Glyoxylate + SUC
R00479 0
mmol/g*h
Glyoxylate + AcCoA -> MAL
R00472 0
mmol/g*h
6-PG -> G3P + PYR
R01064 mmol/g*h
AcCoA -> Acetate
R00227 0
mmol/g*h
PYR -> Lactate
R00704 0
mmol/g*h
AcCoA -> Ethanol
0
mmol/g*h
G6P -> (Cell synthesis)
0.05
mmol/g*h
F6P -> (Cell synthesis)
0.02
mmol/g*h
R5P -> (Cell synthesis)
0.2
mmol/g*h
E4P -> (Cell synthesis)
0.09
mmol/g*h
G3P -> (Cell synthesis)
0.03
mmol/g*h
3PG -> (Cell synthesis)
0.3
mmol/g*h
PEP -> (Cell synthesis)
0.11
mmol/g*h
PYR -> (Cell synthesis)
0.61
mmol/g*h
AcCoA -> (Cell synthesis)
0.82
mmol/g*h
OAA -> (Cell synthesis)
0.4
mmol/g*h
2KG -> (Cell synthesis)
0.24
mmol/g*h
CO2 -> (Evolution)
8.71
mmol/g*h
Exch. (G6P F6P)
0.03
mmol/g*h
Exch. (3PG PEP)
0.03
mmol/g*h
Exch. (R5P + X5P S7P + G3P)
0
mmol/g*h
Exch. (S7P + G3P E4P + F6P)
0.02
mmol/g*h
Exch. (X5P + E4P F6P + G3P)
0
mmol/g*h
Exch. (MAL OAA)
0.03
mmol/g*h
Exch. (PEP + CO2 OAA)
0.00999
mmol/g*h
Glucose consumption rate
2.99797434166104
mmol/g*h
Oxygen uptake rate (OUR)
9.952734638757596
mmol/g*h
Carbon dioxide evolution rate (CER)
4.105334233625928
mmol/g*h
Ethanol production rate
0.0
mmol/g*h
Acetate production rate
0.0008235964392919314
mmol/g*h
D-Lactate production rate
0.0
mmol/g*h
Succinate production rate
0.0
mmol/g*h
Pyruvate production rate
0.0005464256010389427
mmol/g*h
Formate production rate
0.0
mmol/g*h
Graph Loading...
Reaction
KEGG ID
Flux
Unit
Glucose + PEP -> G6P + PYR
R02738 3.18
mmol/g*h
G6P F6P
R02740 3.14
mmol/g*h
F6P -> F1,6P
R04779 2.95
mmol/g*h
F1,6P -> DHAP + G3P
R01070 2.95
mmol/g*h
DHAP -> G3P
R01015 2.95
mmol/g*h
G3P -> 3PG
R01061 5.75
mmol/g*h
3PG PEP
5.45
mmol/g*h
PEP -> PYR
R00200 2.16
mmol/g*h
PYR -> AcCoA + CO2
R00209 4.96
mmol/g*h
G6P -> 6PG
0
mmol/g*h
6PG -> Ru5P + CO2
R01528 0
mmol/g*h
Ru5P -> X5P
R01529 -0.16
mmol/g*h
Ru5P -> R5P
R01056 0.16
mmol/g*h
R5P + X5P S7P + G3P
R01641 -0.04
mmol/g*h
S7P + G3P E4P + F6P
R01827 -0.04
mmol/g*h
X5P + E4P F6P + G3P
R01830 -0.13
mmol/g*h
AcCoA + OAA -> CIT
R00351 3.27
mmol/g*h
CIT -> ICT
R01324 3.27
mmol/g*h
ICT -> 2-KG + CO2
R00267 2.4
mmol/g*h
2-KG -> SUC + CO2
R08549 2.16
mmol/g*h
SUC -> FUM
R00412 3.03
mmol/g*h
FUM -> MAL
R01082 3.03
mmol/g*h
MAL OAA
R00342 3.66
mmol/g*h
PEP + CO2 OAA
R00341 0
mmol/g*h
MAL -> PYR + CO2
R00214 0.23
mmol/g*h
ICT -> Glyoxylate + SUC
R00479 0.87
mmol/g*h
Glyoxylate + AcCoA -> MAL
R00472 0.87
mmol/g*h
6-PG -> G3P + PYR
R01064 mmol/g*h
AcCoA -> Acetate
R00227 0
mmol/g*h
PYR -> Lactate
R00704 0
mmol/g*h
AcCoA -> Ethanol
0.01
mmol/g*h
G6P -> (Cell synthesis)
0.04
mmol/g*h
F6P -> (Cell synthesis)
0.02
mmol/g*h
R5P -> (Cell synthesis)
0.2
mmol/g*h
E4P -> (Cell synthesis)
0.09
mmol/g*h
G3P -> (Cell synthesis)
0.03
mmol/g*h
3PG -> (Cell synthesis)
0.3
mmol/g*h
PEP -> (Cell synthesis)
0.11
mmol/g*h
PYR -> (Cell synthesis)
0.61
mmol/g*h
AcCoA -> (Cell synthesis)
0.82
mmol/g*h
OAA -> (Cell synthesis)
0.39
mmol/g*h
2KG -> (Cell synthesis)
0.24
mmol/g*h
CO2 -> (Evolution)
9.75
mmol/g*h
Exch. (G6P F6P)
0
mmol/g*h
Exch. (3PG PEP)
0.03
mmol/g*h
Exch. (R5P + X5P S7P + G3P)
0
mmol/g*h
Exch. (S7P + G3P E4P + F6P)
0.01
mmol/g*h
Exch. (X5P + E4P F6P + G3P)
0
mmol/g*h
Exch. (MAL OAA)
0.03
mmol/g*h
Exch. (PEP + CO2 OAA)
0.0114798
mmol/g*h
Glucose consumption rate
3.178003966833232
mmol/g*h
Oxygen uptake rate (OUR)
8.764769065521081
mmol/g*h
Carbon dioxide evolution rate (CER)
5.155746509130048
mmol/g*h
Ethanol production rate
0.01360839538767558
mmol/g*h
Acetate production rate
2.903604875729586e-05
mmol/g*h
D-Lactate production rate
0.0
mmol/g*h
Succinate production rate
0.0
mmol/g*h
Pyruvate production rate
0.0006149678626188129
mmol/g*h
Formate production rate
0.0
mmol/g*h
Graph Loading...
Reaction
KEGG ID
Flux
Unit
Glucose + PEP -> G6P + PYR
R02738 3.36
mmol/g*h
G6P F6P
R02740 2.62
mmol/g*h
F6P -> F1,6P
R04779 2.87
mmol/g*h
F1,6P -> DHAP + G3P
R01070 2.87
mmol/g*h
DHAP -> G3P
R01015 2.87
mmol/g*h
G3P -> 3PG
R01061 5.8
mmol/g*h
3PG PEP
5.45
mmol/g*h
PEP -> PYR
R00200 1.73
mmol/g*h
PYR -> AcCoA + CO2
R00209 4.39
mmol/g*h
G6P -> 6PG
0.69
mmol/g*h
6PG -> Ru5P + CO2
R01528 0.69
mmol/g*h
Ru5P -> X5P
R01529 0.27
mmol/g*h
Ru5P -> R5P
R01056 0.41
mmol/g*h
R5P + X5P S7P + G3P
R01641 0.19
mmol/g*h
S7P + G3P E4P + F6P
R01827 0.19
mmol/g*h
X5P + E4P F6P + G3P
R01830 0.09
mmol/g*h
AcCoA + OAA -> CIT
R00351 2.95
mmol/g*h
CIT -> ICT
R01324 2.95
mmol/g*h
ICT -> 2-KG + CO2
R00267 2.46
mmol/g*h
2-KG -> SUC + CO2
R08549 2.19
mmol/g*h
SUC -> FUM
R00412 2.68
mmol/g*h
FUM -> MAL
R01082 2.68
mmol/g*h
MAL OAA
R00342 3.17
mmol/g*h
PEP + CO2 OAA
R00341 0.24
mmol/g*h
MAL -> PYR + CO2
R00214 0
mmol/g*h
ICT -> Glyoxylate + SUC
R00479 0.49
mmol/g*h
Glyoxylate + AcCoA -> MAL
R00472 0.49
mmol/g*h
6-PG -> G3P + PYR
R01064 0
mmol/g*h
AcCoA -> Acetate
R00227 0
mmol/g*h
PYR -> Lactate
R00704 0
mmol/g*h
AcCoA -> Ethanol
0
mmol/g*h
G6P -> (Cell synthesis)
0.05
mmol/g*h
F6P -> (Cell synthesis)
0.02
mmol/g*h
R5P -> (Cell synthesis)
0.23
mmol/g*h
E4P -> (Cell synthesis)
0.1
mmol/g*h
G3P -> (Cell synthesis)
0.04
mmol/g*h
3PG -> (Cell synthesis)
0.34
mmol/g*h
PEP -> (Cell synthesis)
0.12
mmol/g*h
PYR -> (Cell synthesis)
0.7
mmol/g*h
AcCoA -> (Cell synthesis)
0.94
mmol/g*h
OAA -> (Cell synthesis)
0.45
mmol/g*h
2KG -> (Cell synthesis)
0.28
mmol/g*h
CO2 -> (Evolution)
9.48
mmol/g*h
Exch. (G6P F6P)
0.03
mmol/g*h
Exch. (3PG PEP)
0.03
mmol/g*h
Exch. (R5P + X5P S7P + G3P)
0
mmol/g*h
Exch. (S7P + G3P E4P + F6P)
0.02
mmol/g*h
Exch. (X5P + E4P F6P + G3P)
0
mmol/g*h
Exch. (MAL OAA)
0.03
mmol/g*h
Exch. (PEP + CO2 OAA)
0.0085008
mmol/g*h
Glucose consumption rate
3.361309989602278
mmol/g*h
Oxygen uptake rate (OUR)
11.866717152593624
mmol/g*h
Carbon dioxide evolution rate (CER)
7.088224157516038
mmol/g*h
Ethanol production rate
0.0
mmol/g*h
Acetate production rate
0.0021863769019778967
mmol/g*h
D-Lactate production rate
0.00048466489965921485
mmol/g*h
Succinate production rate
0.0012545907491396885
mmol/g*h
Pyruvate production rate
0.0
mmol/g*h
Formate production rate
0.002920771152501533
mmol/g*h
Graph Loading...
Reaction
KEGG ID
Flux
Unit
Glucose + PEP -> G6P + PYR
R02738 2.92
mmol/g*h
G6P F6P
R02740 2.88
mmol/g*h
F6P -> F1,6P
R04779 2.73
mmol/g*h
F1,6P -> DHAP + G3P
R01070 2.73
mmol/g*h
DHAP -> G3P
R01015 2.73
mmol/g*h
G3P -> 3PG
R01061 5.32
mmol/g*h
3PG PEP
5.06
mmol/g*h
PEP -> PYR
R00200 1.95
mmol/g*h
PYR -> AcCoA + CO2
R00209 4.34
mmol/g*h
G6P -> 6PG
0
mmol/g*h
6PG -> Ru5P + CO2
R01528 mmol/g*h
Ru5P -> X5P
R01529 -0.14
mmol/g*h
Ru5P -> R5P
R01056 0.14
mmol/g*h
R5P + X5P S7P + G3P
R01641 -0.03
mmol/g*h
S7P + G3P E4P + F6P
R01827 -0.03
mmol/g*h
X5P + E4P F6P + G3P
R01830 -0.11
mmol/g*h
AcCoA + OAA -> CIT
R00351 3.17
mmol/g*h
CIT -> ICT
R01324 3.17
mmol/g*h
ICT -> 2-KG + CO2
R00267 2.7
mmol/g*h
2-KG -> SUC + CO2
R08549 2.49
mmol/g*h
SUC -> FUM
R00412 2.96
mmol/g*h
FUM -> MAL
R01082 2.96
mmol/g*h
MAL OAA
R00342 3.42
mmol/g*h
PEP + CO2 OAA
R00341 0.09
mmol/g*h
MAL -> PYR + CO2
R00214 0
mmol/g*h
ICT -> Glyoxylate + SUC
R00479 0.46
mmol/g*h
Glyoxylate + AcCoA -> MAL
R00472 0.46
mmol/g*h
6-PG -> G3P + PYR
R01064 0
mmol/g*h
AcCoA -> Acetate
R00227 0
mmol/g*h
PYR -> Lactate
R00704 0
mmol/g*h
AcCoA -> Ethanol
0
mmol/g*h
G6P -> (Cell synthesis)
0.04
mmol/g*h
F6P -> (Cell synthesis)
0.02
mmol/g*h
R5P -> (Cell synthesis)
0.17
mmol/g*h
E4P -> (Cell synthesis)
0.08
mmol/g*h
G3P -> (Cell synthesis)
0.03
mmol/g*h
3PG -> (Cell synthesis)
0.26
mmol/g*h
PEP -> (Cell synthesis)
0.09
mmol/g*h
PYR -> (Cell synthesis)
0.53
mmol/g*h
AcCoA -> (Cell synthesis)
0.71
mmol/g*h
OAA -> (Cell synthesis)
0.34
mmol/g*h
2KG -> (Cell synthesis)
0.21
mmol/g*h
CO2 -> (Evolution)
9.45
mmol/g*h
Exch. (G6P F6P)
0
mmol/g*h
Exch. (3PG PEP)
0.03
mmol/g*h
Exch. (R5P + X5P S7P + G3P)
0.02
mmol/g*h
Exch. (S7P + G3P E4P + F6P)
0
mmol/g*h
Exch. (X5P + E4P F6P + G3P)
0
mmol/g*h
Exch. (MAL OAA)
0.02
mmol/g*h
Exch. (PEP + CO2 OAA)
0.0098696
mmol/g*h
Glucose consumption rate
2.9219556057303913
mmol/g*h
Oxygen uptake rate (OUR)
6.005926901547595
mmol/g*h
Carbon dioxide evolution rate (CER)
4.109318406322039
mmol/g*h
Ethanol production rate
0.0011856376093443907
mmol/g*h
Acetate production rate
0.0006669806430604876
mmol/g*h
D-Lactate production rate
0.0
mmol/g*h
Succinate production rate
0.0002567035801678537
mmol/g*h
Pyruvate production rate
0.00024797623843564337
mmol/g*h
Formate production rate
0.003404989259450908
mmol/g*h
Graph Loading...
Reaction
KEGG ID
Flux
Unit
Glucose + PEP -> G6P + PYR
R02738 3.63
mmol/g*h
G6P F6P
R02740 3.35
mmol/g*h
F6P -> F1,6P
R04779 3.33
mmol/g*h
F1,6P -> DHAP + G3P
R01070 3.33
mmol/g*h
DHAP -> G3P
R01015 3.33
mmol/g*h
G3P -> 3PG
R01061 6.57
mmol/g*h
3PG PEP
6.28
mmol/g*h
PEP -> PYR
R00200 2.54
mmol/g*h
PYR -> AcCoA + CO2
R00209 5.55
mmol/g*h
G6P -> 6PG
0.24
mmol/g*h
6PG -> Ru5P + CO2
R01528 0.24
mmol/g*h
Ru5P -> X5P
R01529 mmol/g*h
Ru5P -> R5P
R01056 0.24
mmol/g*h
R5P + X5P S7P + G3P
R01641 0.04
mmol/g*h
S7P + G3P E4P + F6P
R01827 0.04
mmol/g*h
X5P + E4P F6P + G3P
R01830 -0.04
mmol/g*h
AcCoA + OAA -> CIT
R00351 4.04
mmol/g*h
CIT -> ICT
R01324 4.04
mmol/g*h
ICT -> 2-KG + CO2
R00267 3.41
mmol/g*h
2-KG -> SUC + CO2
R08549 3.17
mmol/g*h
SUC -> FUM
R00412 3.8
mmol/g*h
FUM -> MAL
R01082 3.8
mmol/g*h
MAL OAA
R00342 4.44
mmol/g*h
PEP + CO2 OAA
R00341 0
mmol/g*h
MAL -> PYR + CO2
R00214 0
mmol/g*h
ICT -> Glyoxylate + SUC
R00479 0.64
mmol/g*h
Glyoxylate + AcCoA -> MAL
R00472 0.64
mmol/g*h
6-PG -> G3P + PYR
R01064 0
mmol/g*h
AcCoA -> Acetate
R00227 0
mmol/g*h
PYR -> Lactate
R00704 0.01
mmol/g*h
AcCoA -> Ethanol
0.05
mmol/g*h
G6P -> (Cell synthesis)
0.04
mmol/g*h
F6P -> (Cell synthesis)
0.02
mmol/g*h
R5P -> (Cell synthesis)
0.2
mmol/g*h
E4P -> (Cell synthesis)
0.09
mmol/g*h
G3P -> (Cell synthesis)
0.03
mmol/g*h
3PG -> (Cell synthesis)
0.3
mmol/g*h
PEP -> (Cell synthesis)
0.11
mmol/g*h
PYR -> (Cell synthesis)
0.61
mmol/g*h
AcCoA -> (Cell synthesis)
0.82
mmol/g*h
OAA -> (Cell synthesis)
0.4
mmol/g*h
2KG -> (Cell synthesis)
0.24
mmol/g*h
CO2 -> (Evolution)
12.37
mmol/g*h
Exch. (G6P F6P)
0
mmol/g*h
Exch. (3PG PEP)
0.03
mmol/g*h
Exch. (R5P + X5P S7P + G3P)
0.03
mmol/g*h
Exch. (S7P + G3P E4P + F6P)
0.01
mmol/g*h
Exch. (X5P + E4P F6P + G3P)
0
mmol/g*h
Exch. (MAL OAA)
0.03
mmol/g*h
Exch. (PEP + CO2 OAA)
0.0092928
mmol/g*h
Glucose consumption rate
3.632823006780454
mmol/g*h
Oxygen uptake rate (OUR)
8.036006546644845
mmol/g*h
Carbon dioxide evolution rate (CER)
6.317512274959084
mmol/g*h
Ethanol production rate
0.053066617120392824
mmol/g*h
Acetate production rate
0.0013914242190506093
mmol/g*h
D-Lactate production rate
0.0
mmol/g*h
Succinate production rate
0.0037215492720039122
mmol/g*h
Pyruvate production rate
0.0014190840223380899
mmol/g*h
Formate production rate
0.00592606709801324
mmol/g*h
Graph Loading...
Reaction
KEGG ID
Flux
Unit
Glucose + PEP -> G6P + PYR
R02738 3.18
mmol/g*h
G6P F6P
R02740 2.14
mmol/g*h
F6P -> F1,6P
R04779 2.21
mmol/g*h
F1,6P -> DHAP + G3P
R01070 2.21
mmol/g*h
DHAP -> G3P
R01015 2.21
mmol/g*h
G3P -> 3PG
R01061 5
mmol/g*h
3PG PEP
4.71
mmol/g*h
PEP -> PYR
R00200 1.42
mmol/g*h
PYR -> AcCoA + CO2
R00209 4.6
mmol/g*h
G6P -> 6PG
1
mmol/g*h
6PG -> Ru5P + CO2
R01528 0.38
mmol/g*h
Ru5P -> X5P
R01529 0.09
mmol/g*h
Ru5P -> R5P
R01056 0.29
mmol/g*h
R5P + X5P S7P + G3P
R01641 0.09
mmol/g*h
S7P + G3P E4P + F6P
R01827 0.09
mmol/g*h
X5P + E4P F6P + G3P
R01830 0
mmol/g*h
AcCoA + OAA -> CIT
R00351 3.15
mmol/g*h
CIT -> ICT
R01324 3.15
mmol/g*h
ICT -> 2-KG + CO2
R00267 2.52
mmol/g*h
2-KG -> SUC + CO2
R08549 2.27
mmol/g*h
SUC -> FUM
R00412 2.91
mmol/g*h
FUM -> MAL
R01082 2.91
mmol/g*h
MAL OAA
R00342 3.55
mmol/g*h
PEP + CO2 OAA
R00341 0
mmol/g*h
MAL -> PYR + CO2
R00214 0
mmol/g*h
ICT -> Glyoxylate + SUC
R00479 0.64
mmol/g*h
Glyoxylate + AcCoA -> MAL
R00472 0.64
mmol/g*h
6-PG -> G3P + PYR
R01064 0.62
mmol/g*h
AcCoA -> Acetate
R00227 0
mmol/g*h
PYR -> Lactate
R00704 0
mmol/g*h
AcCoA -> Ethanol
0
mmol/g*h
G6P -> (Cell synthesis)
0.04
mmol/g*h
F6P -> (Cell synthesis)
0.02
mmol/g*h
R5P -> (Cell synthesis)
0.2
mmol/g*h
E4P -> (Cell synthesis)
0.09
mmol/g*h
G3P -> (Cell synthesis)
0.03
mmol/g*h
3PG -> (Cell synthesis)
0.3
mmol/g*h
PEP -> (Cell synthesis)
0.11
mmol/g*h
PYR -> (Cell synthesis)
0.61
mmol/g*h
AcCoA -> (Cell synthesis)
0.82
mmol/g*h
OAA -> (Cell synthesis)
0.39
mmol/g*h
2KG -> (Cell synthesis)
0.24
mmol/g*h
CO2 -> (Evolution)
9.77
mmol/g*h
Exch. (G6P F6P)
0.01
mmol/g*h
Exch. (3PG PEP)
0.03
mmol/g*h
Exch. (R5P + X5P S7P + G3P)
0.01
mmol/g*h
Exch. (S7P + G3P E4P + F6P)
0.02
mmol/g*h
Exch. (X5P + E4P F6P + G3P)
0.01
mmol/g*h
Exch. (MAL OAA)
0.03
mmol/g*h
Exch. (PEP + CO2 OAA)
0.0129108
mmol/g*h
Glucose consumption rate
3.1848870208052125
mmol/g*h
Oxygen uptake rate (OUR)
3.021169716541084
mmol/g*h
Carbon dioxide evolution rate (CER)
6.207391460351633
mmol/g*h
Ethanol production rate
0.0
mmol/g*h
Acetate production rate
0.0008330016668650543
mmol/g*h
D-Lactate production rate
0.0
mmol/g*h
Succinate production rate
0.0
mmol/g*h
Pyruvate production rate
0.0010903624113816706
mmol/g*h
Formate production rate
0.0011535037010458585
mmol/g*h
Graph Loading...
Reaction
KEGG ID
Flux
Unit
Glucose + PEP -> G6P + PYR
R02738 4.15
mmol/g*h
G6P F6P
R02740 2.11
mmol/g*h
F6P -> F1,6P
R04779 3.26
mmol/g*h
F1,6P -> DHAP + G3P
R01070 3.26
mmol/g*h
DHAP -> G3P
R01015 3.26
mmol/g*h
G3P -> 3PG
R01061 7.03
mmol/g*h
3PG PEP
6.73
mmol/g*h
PEP -> PYR
R00200 2.18
mmol/g*h
PYR -> AcCoA + CO2
R00209 5.72
mmol/g*h
G6P -> 6PG
2
mmol/g*h
6PG -> Ru5P + CO2
R01528 2
mmol/g*h
Ru5P -> X5P
R01529 1.17
mmol/g*h
Ru5P -> R5P
R01056 0.82
mmol/g*h
R5P + X5P S7P + G3P
R01641 0.63
mmol/g*h
S7P + G3P E4P + F6P
R01827 0.63
mmol/g*h
X5P + E4P F6P + G3P
R01830 0.54
mmol/g*h
AcCoA + OAA -> CIT
R00351 4.55
mmol/g*h
CIT -> ICT
R01324 4.55
mmol/g*h
ICT -> 2-KG + CO2
R00267 4.2
mmol/g*h
2-KG -> SUC + CO2
R08549 3.96
mmol/g*h
SUC -> FUM
R00412 4.3
mmol/g*h
FUM -> MAL
R01082 4.3
mmol/g*h
MAL OAA
R00342 4.64
mmol/g*h
PEP + CO2 OAA
R00341 0.29
mmol/g*h
MAL -> PYR + CO2
R00214 0.01
mmol/g*h
ICT -> Glyoxylate + SUC
R00479 0.35
mmol/g*h
Glyoxylate + AcCoA -> MAL
R00472 0.35
mmol/g*h
6-PG -> G3P + PYR
R01064 mmol/g*h
AcCoA -> Acetate
R00227 0
mmol/g*h
PYR -> Lactate
R00704 0
mmol/g*h
AcCoA -> Ethanol
0.01
mmol/g*h
G6P -> (Cell synthesis)
0.05
mmol/g*h
F6P -> (Cell synthesis)
0.02
mmol/g*h
R5P -> (Cell synthesis)
0.2
mmol/g*h
E4P -> (Cell synthesis)
0.09
mmol/g*h
G3P -> (Cell synthesis)
0.03
mmol/g*h
3PG -> (Cell synthesis)
0.3
mmol/g*h
PEP -> (Cell synthesis)
0.11
mmol/g*h
PYR -> (Cell synthesis)
0.61
mmol/g*h
AcCoA -> (Cell synthesis)
0.82
mmol/g*h
OAA -> (Cell synthesis)
0.39
mmol/g*h
2KG -> (Cell synthesis)
0.24
mmol/g*h
CO2 -> (Evolution)
15.59
mmol/g*h
Exch. (G6P F6P)
0.04
mmol/g*h
Exch. (3PG PEP)
0.04
mmol/g*h
Exch. (R5P + X5P S7P + G3P)
0.04
mmol/g*h
Exch. (S7P + G3P E4P + F6P)
0.02
mmol/g*h
Exch. (X5P + E4P F6P + G3P)
0.01
mmol/g*h
Exch. (MAL OAA)
0.04
mmol/g*h
Exch. (PEP + CO2 OAA)
0.016351
mmol/g*h
Glucose consumption rate
4.14784821243139
mmol/g*h
Oxygen uptake rate (OUR)
15.523364485981308
mmol/g*h
Carbon dioxide evolution rate (CER)
6.990654205607477
mmol/g*h
Ethanol production rate
0.013554343349920582
mmol/g*h
Acetate production rate
0.0
mmol/g*h
D-Lactate production rate
0.0
mmol/g*h
Succinate production rate
0.007438157900406344
mmol/g*h
Pyruvate production rate
0.0017750233881546184
mmol/g*h
Formate production rate
0.0003619641002818576
mmol/g*h
Graph Loading...
Reaction
KEGG ID
Flux
Unit
Glucose + PEP -> G6P + PYR
R02738 4.49
mmol/g*h
G6P F6P
R02740 3.49
mmol/g*h
F6P -> F1,6P
R04779 3.56
mmol/g*h
F1,6P -> DHAP + G3P
R01070 3.56
mmol/g*h
DHAP -> G3P
R01015 3.56
mmol/g*h
G3P -> 3PG
R01061 7.66
mmol/g*h
3PG PEP
7.37
mmol/g*h
PEP -> PYR
R00200 2.13
mmol/g*h
PYR -> AcCoA + CO2
R00209 6.59
mmol/g*h
G6P -> 6PG
0.95
mmol/g*h
6PG -> Ru5P + CO2
R01528 0.38
mmol/g*h
Ru5P -> X5P
R01529 0.09
mmol/g*h
Ru5P -> R5P
R01056 0.29
mmol/g*h
R5P + X5P S7P + G3P
R01641 0.09
mmol/g*h
S7P + G3P E4P + F6P
R01827 0.09
mmol/g*h
X5P + E4P F6P + G3P
R01830 0
mmol/g*h
AcCoA + OAA -> CIT
R00351 5.77
mmol/g*h
CIT -> ICT
R01324 5.77
mmol/g*h
ICT -> 2-KG + CO2
R00267 5.77
mmol/g*h
2-KG -> SUC + CO2
R08549 5.53
mmol/g*h
SUC -> FUM
R00412 5.53
mmol/g*h
FUM -> MAL
R01082 5.53
mmol/g*h
MAL OAA
R00342 5.53
mmol/g*h
PEP + CO2 OAA
R00341 0.64
mmol/g*h
MAL -> PYR + CO2
R00214 0
mmol/g*h
ICT -> Glyoxylate + SUC
R00479 0
mmol/g*h
Glyoxylate + AcCoA -> MAL
R00472 0
mmol/g*h
6-PG -> G3P + PYR
R01064 0.57
mmol/g*h
AcCoA -> Acetate
R00227 0
mmol/g*h
PYR -> Lactate
R00704 0
mmol/g*h
AcCoA -> Ethanol
0
mmol/g*h
G6P -> (Cell synthesis)
0.04
mmol/g*h
F6P -> (Cell synthesis)
0.02
mmol/g*h
R5P -> (Cell synthesis)
0.2
mmol/g*h
E4P -> (Cell synthesis)
0.09
mmol/g*h
G3P -> (Cell synthesis)
0.03
mmol/g*h
3PG -> (Cell synthesis)
0.3
mmol/g*h
PEP -> (Cell synthesis)
0.11
mmol/g*h
PYR -> (Cell synthesis)
0.61
mmol/g*h
AcCoA -> (Cell synthesis)
0.82
mmol/g*h
OAA -> (Cell synthesis)
0.4
mmol/g*h
2KG -> (Cell synthesis)
0.24
mmol/g*h
CO2 -> (Evolution)
17.62
mmol/g*h
Exch. (G6P F6P)
0.04
mmol/g*h
Exch. (3PG PEP)
0.04
mmol/g*h
Exch. (R5P + X5P S7P + G3P)
0
mmol/g*h
Exch. (S7P + G3P E4P + F6P)
0.02
mmol/g*h
Exch. (X5P + E4P F6P + G3P)
0
mmol/g*h
Exch. (MAL OAA)
0.04
mmol/g*h
Exch. (PEP + CO2 OAA)
0.0162987
mmol/g*h
Glucose consumption rate
4.492772379667117
mmol/g*h
Oxygen uptake rate (OUR)
8.997975708502025
mmol/g*h
Carbon dioxide evolution rate (CER)
7.57085020242915
mmol/g*h
Ethanol production rate
0.0
mmol/g*h
Acetate production rate
0.0019234322476806
mmol/g*h
D-Lactate production rate
0.0
mmol/g*h
Succinate production rate
0.0
mmol/g*h
Pyruvate production rate
0.00125370204980996
mmol/g*h
Formate production rate
0.0011596833965255
mmol/g*h
Graph Loading...
Reaction
KEGG ID
Flux
Unit
Glucose + PEP -> G6P + PYR
R02738 2.97
mmol/g*h
G6P F6P
R02740 2.55
mmol/g*h
F6P -> F1,6P
R04779 2.62
mmol/g*h
F1,6P -> DHAP + G3P
R01070 2.62
mmol/g*h
DHAP -> G3P
R01015 2.62
mmol/g*h
G3P -> 3PG
R01061 5.21
mmol/g*h
3PG PEP
4.91
mmol/g*h
PEP -> PYR
R00200 1.2
mmol/g*h
PYR -> AcCoA + CO2
R00209 3.56
mmol/g*h
G6P -> 6PG
0.37
mmol/g*h
6PG -> Ru5P + CO2
R01528 0.37
mmol/g*h
Ru5P -> X5P
R01529 0.09
mmol/g*h
Ru5P -> R5P
R01056 0.29
mmol/g*h
R5P + X5P S7P + G3P
R01641 0.09
mmol/g*h
S7P + G3P E4P + F6P
R01827 0.09
mmol/g*h
X5P + E4P F6P + G3P
R01830 0
mmol/g*h
AcCoA + OAA -> CIT
R00351 2.74
mmol/g*h
CIT -> ICT
R01324 2.74
mmol/g*h
ICT -> 2-KG + CO2
R00267 2.74
mmol/g*h
2-KG -> SUC + CO2
R08549 2.5
mmol/g*h
SUC -> FUM
R00412 2.5
mmol/g*h
FUM -> MAL
R01082 2.5
mmol/g*h
MAL OAA
R00342 2.5
mmol/g*h
PEP + CO2 OAA
R00341 0.64
mmol/g*h
MAL -> PYR + CO2
R00214 0
mmol/g*h
ICT -> Glyoxylate + SUC
R00479 0
mmol/g*h
Glyoxylate + AcCoA -> MAL
R00472 0
mmol/g*h
6-PG -> G3P + PYR
R01064 mmol/g*h
AcCoA -> Acetate
R00227 0
mmol/g*h
PYR -> Lactate
R00704 0
mmol/g*h
AcCoA -> Ethanol
0
mmol/g*h
G6P -> (Cell synthesis)
0.04
mmol/g*h
F6P -> (Cell synthesis)
0.02
mmol/g*h
R5P -> (Cell synthesis)
0.2
mmol/g*h
E4P -> (Cell synthesis)
0.09
mmol/g*h
G3P -> (Cell synthesis)
0.03
mmol/g*h
3PG -> (Cell synthesis)
0.3
mmol/g*h
PEP -> (Cell synthesis)
0.11
mmol/g*h
PYR -> (Cell synthesis)
0.61
mmol/g*h
AcCoA -> (Cell synthesis)
0.82
mmol/g*h
OAA -> (Cell synthesis)
0.4
mmol/g*h
2KG -> (Cell synthesis)
0.24
mmol/g*h
CO2 -> (Evolution)
8.53
mmol/g*h
Exch. (G6P F6P)
0.03
mmol/g*h
Exch. (3PG PEP)
0.03
mmol/g*h
Exch. (R5P + X5P S7P + G3P)
0
mmol/g*h
Exch. (S7P + G3P E4P + F6P)
0.02
mmol/g*h
Exch. (X5P + E4P F6P + G3P)
0
mmol/g*h
Exch. (MAL OAA)
0.03
mmol/g*h
Exch. (PEP + CO2 OAA)
0.0116127
mmol/g*h
Glucose consumption rate
2.968758167436469
mmol/g*h
Oxygen uptake rate (OUR)
12.207357859531939
mmol/g*h
Carbon dioxide evolution rate (CER)
5.946488294314462
mmol/g*h
Ethanol production rate
0.0
mmol/g*h
Acetate production rate
0.0
mmol/g*h
D-Lactate production rate
0.0
mmol/g*h
Succinate production rate
0.0
mmol/g*h
Pyruvate production rate
0.0005634027994276486
mmol/g*h
Formate production rate
0.0005828953655040661
mmol/g*h
Graph Loading...
Reaction
KEGG ID
Flux
Unit
Glucose + PEP -> G6P + PYR
R02738 2.8
mmol/g*h
G6P F6P
R02740 2.39
mmol/g*h
F6P -> F1,6P
R04779 2.45
mmol/g*h
F1,6P -> DHAP + G3P
R01070 2.45
mmol/g*h
DHAP -> G3P
R01015 2.45
mmol/g*h
G3P -> 3PG
R01061 4.87
mmol/g*h
3PG PEP
4.57
mmol/g*h
PEP -> PYR
R00200 1.09
mmol/g*h
PYR -> AcCoA + CO2
R00209 3.28
mmol/g*h
G6P -> 6PG
0.37
mmol/g*h
6PG -> Ru5P + CO2
R01528 0.37
mmol/g*h
Ru5P -> X5P
R01529 0.09
mmol/g*h
Ru5P -> R5P
R01056 0.28
mmol/g*h
R5P + X5P S7P + G3P
R01641 0.09
mmol/g*h
S7P + G3P E4P + F6P
R01827 0.09
mmol/g*h
X5P + E4P F6P + G3P
R01830 0
mmol/g*h
AcCoA + OAA -> CIT
R00351 2.4
mmol/g*h
CIT -> ICT
R01324 2.4
mmol/g*h
ICT -> 2-KG + CO2
R00267 2.33
mmol/g*h
2-KG -> SUC + CO2
R08549 2.09
mmol/g*h
SUC -> FUM
R00412 2.16
mmol/g*h
FUM -> MAL
R01082 2.16
mmol/g*h
MAL OAA
R00342 2.22
mmol/g*h
PEP + CO2 OAA
R00341 0.57
mmol/g*h
MAL -> PYR + CO2
R00214 0
mmol/g*h
ICT -> Glyoxylate + SUC
R00479 0.07
mmol/g*h
Glyoxylate + AcCoA -> MAL
R00472 0.07
mmol/g*h
6-PG -> G3P + PYR
R01064
AcCoA -> Acetate
R00227 0
mmol/g*h
PYR -> Lactate
R00704 0
mmol/g*h
AcCoA -> Ethanol
0
mmol/g*h
G6P -> (Cell synthesis)
0.04
mmol/g*h
F6P -> (Cell synthesis)
0.02
mmol/g*h
R5P -> (Cell synthesis)
0.2
mmol/g*h
E4P -> (Cell synthesis)
0.09
mmol/g*h
G3P -> (Cell synthesis)
0.03
mmol/g*h
3PG -> (Cell synthesis)
0.3
mmol/g*h
PEP -> (Cell synthesis)
0.11
mmol/g*h
PYR -> (Cell synthesis)
0.61
mmol/g*h
AcCoA -> (Cell synthesis)
0.82
mmol/g*h
OAA -> (Cell synthesis)
0.39
mmol/g*h
2KG -> (Cell synthesis)
0.24
mmol/g*h
CO2 -> (Evolution)
7.51
mmol/g*h
Exch. (G6P F6P)
0.02
mmol/g*h
Exch. (3PG PEP)
0.03
mmol/g*h
Exch. (R5P + X5P S7P + G3P)
0
mmol/g*h
Exch. (S7P + G3P E4P + F6P)
0.02
mmol/g*h
Exch. (X5P + E4P F6P + G3P)
0
mmol/g*h
Exch. (MAL OAA)
0.03
mmol/g*h
Exch. (PEP + CO2 OAA)
0.01344
mmol/g*h
Glucose consumption rate
2.8035473332731606
mmol/g*h
Oxygen uptake rate (OUR)
3.3986102337334057
mmol/g*h
Carbon dioxide evolution rate (CER)
5.912823752368899
mmol/g*h
Ethanol production rate
0.0
mmol/g*h
Acetate production rate
0.0006189584105021452
mmol/g*h
D-Lactate production rate
0.0004342434664607717
mmol/g*h
Succinate production rate
0.0014569461109593354
mmol/g*h
Pyruvate production rate
0.0
mmol/g*h
Formate production rate
6.116575920745034e-05
mmol/g*h
Graph Loading...
Reaction
KEGG ID
Flux
Unit
Glucose + PEP -> G6P + PYR
R02738 2.94
mmol/g*h
G6P F6P
R02740 2.52
mmol/g*h
F6P -> F1,6P
R04779 2.59
mmol/g*h
F1,6P -> DHAP + G3P
R01070 2.59
mmol/g*h
DHAP -> G3P
R01015 2.59
mmol/g*h
G3P -> 3PG
R01061 5.15
mmol/g*h
3PG PEP
4.85
mmol/g*h
PEP -> PYR
R00200 1.22
mmol/g*h
PYR -> AcCoA + CO2
R00209 3.55
mmol/g*h
G6P -> 6PG
0.37
mmol/g*h
6PG -> Ru5P + CO2
R01528 0.37
mmol/g*h
Ru5P -> X5P
R01529 0.09
mmol/g*h
Ru5P -> R5P
R01056 0.29
mmol/g*h
R5P + X5P S7P + G3P
R01641 0.09
mmol/g*h
S7P + G3P E4P + F6P
R01827 0.09
mmol/g*h
X5P + E4P F6P + G3P
R01830 0
mmol/g*h
AcCoA + OAA -> CIT
R00351 2.67
mmol/g*h
CIT -> ICT
R01324 2.67
mmol/g*h
ICT -> 2-KG + CO2
R00267 2.62
mmol/g*h
2-KG -> SUC + CO2
R08549 2.38
mmol/g*h
SUC -> FUM
R00412 2.43
mmol/g*h
FUM -> MAL
R01082 2.43
mmol/g*h
MAL OAA
R00342 2.49
mmol/g*h
PEP + CO2 OAA
R00341 0.58
mmol/g*h
MAL -> PYR + CO2
R00214 0
mmol/g*h
ICT -> Glyoxylate + SUC
R00479 0.06
mmol/g*h
Glyoxylate + AcCoA -> MAL
R00472 0.06
mmol/g*h
6-PG -> G3P + PYR
R01064 mmol/g*h
AcCoA -> Acetate
R00227 0
mmol/g*h
PYR -> Lactate
R00704 0
mmol/g*h
AcCoA -> Ethanol
0
mmol/g*h
G6P -> (Cell synthesis)
0.04
mmol/g*h
F6P -> (Cell synthesis)
0.02
mmol/g*h
R5P -> (Cell synthesis)
0.2
mmol/g*h
E4P -> (Cell synthesis)
0.09
mmol/g*h
G3P -> (Cell synthesis)
0.03
mmol/g*h
3PG -> (Cell synthesis)
0.3
mmol/g*h
PEP -> (Cell synthesis)
0.11
mmol/g*h
PYR -> (Cell synthesis)
0.61
mmol/g*h
AcCoA -> (Cell synthesis)
0.82
mmol/g*h
OAA -> (Cell synthesis)
0.4
mmol/g*h
2KG -> (Cell synthesis)
0.24
mmol/g*h
CO2 -> (Evolution)
8.33
mmol/g*h
Exch. (G6P F6P)
0.01
mmol/g*h
Exch. (3PG PEP)
0.03
mmol/g*h
Exch. (R5P + X5P S7P + G3P)
0
mmol/g*h
Exch. (S7P + G3P E4P + F6P)
0.01
mmol/g*h
Exch. (X5P + E4P F6P + G3P)
0
mmol/g*h
Exch. (MAL OAA)
0.03
mmol/g*h
Exch. (PEP + CO2 OAA)
0.0114072
mmol/g*h
Glucose consumption rate
2.9352461157302425
mmol/g*h
Oxygen uptake rate (OUR)
10.985449735449736
mmol/g*h
Carbon dioxide evolution rate (CER)
6.805555555555555
mmol/g*h
Ethanol production rate
0.0
mmol/g*h
Acetate production rate
0.0007037181367353846
mmol/g*h
D-Lactate production rate
0.0
mmol/g*h
Succinate production rate
0.0013749795121504984
mmol/g*h
Pyruvate production rate
0.0002184581349580685
mmol/g*h
Formate production rate
0.0001600949021583944
mmol/g*h
Graph Loading...
Reaction
KEGG ID
Flux
Unit
Glucose + PEP -> G6P + PYR
R02738 1.34
mmol/g*h
G6P F6P
R02740 0.83
mmol/g*h
F6P -> F1,6P
R04779 1.07
mmol/g*h
F1,6P -> DHAP + G3P
R01070 1.07
mmol/g*h
DHAP -> G3P
R01015 1.07
mmol/g*h
G3P -> 3PG
R01061 2.24
mmol/g*h
3PG PEP
2.1
mmol/g*h
PEP -> PYR
R00200 0.71
mmol/g*h
PYR -> AcCoA + CO2
R00209 1.82
mmol/g*h
G6P -> 6PG
0.49
mmol/g*h
6PG -> Ru5P + CO2
R01528 0.49
mmol/g*h
Ru5P -> X5P
R01529 0.25
mmol/g*h
Ru5P -> R5P
R01056 0.24
mmol/g*h
R5P + X5P S7P + G3P
R01641 0.15
mmol/g*h
S7P + G3P E4P + F6P
R01827 0.15
mmol/g*h
X5P + E4P F6P + G3P
R01830 0.11
mmol/g*h
AcCoA + OAA -> CIT
R00351 1.1
mmol/g*h
CIT -> ICT
R01324 1.1
mmol/g*h
ICT -> 2-KG + CO2
R00267 0.76
mmol/g*h
2-KG -> SUC + CO2
R08549 0.65
mmol/g*h
SUC -> FUM
R00412 0.99
mmol/g*h
FUM -> MAL
R01082 0.99
mmol/g*h
MAL OAA
R00342 1.28
mmol/g*h
PEP + CO2 OAA
R00341 0
mmol/g*h
MAL -> PYR + CO2
R00214 0.05
mmol/g*h
ICT -> Glyoxylate + SUC
R00479 0.34
mmol/g*h
Glyoxylate + AcCoA -> MAL
R00472 0.34
mmol/g*h
6-PG -> G3P + PYR
R01064 mmol/g*h
AcCoA -> Acetate
R00227 0
mmol/g*h
PYR -> Lactate
R00704 0
mmol/g*h
AcCoA -> Ethanol
0
mmol/g*h
G6P -> (Cell synthesis)
0.02
mmol/g*h
F6P -> (Cell synthesis)
0.01
mmol/g*h
R5P -> (Cell synthesis)
0.09
mmol/g*h
E4P -> (Cell synthesis)
0.04
mmol/g*h
G3P -> (Cell synthesis)
0.01
mmol/g*h
3PG -> (Cell synthesis)
0.14
mmol/g*h
PEP -> (Cell synthesis)
0.05
mmol/g*h
PYR -> (Cell synthesis)
0.28
mmol/g*h
AcCoA -> (Cell synthesis)
0.38
mmol/g*h
OAA -> (Cell synthesis)
0.18
mmol/g*h
2KG -> (Cell synthesis)
0.11
mmol/g*h
CO2 -> (Evolution)
3.76
mmol/g*h
Exch. (G6P F6P)
0.01
mmol/g*h
Exch. (3PG PEP)
0.01
mmol/g*h
Exch. (R5P + X5P S7P + G3P)
0
mmol/g*h
Exch. (S7P + G3P E4P + F6P)
0.01
mmol/g*h
Exch. (X5P + E4P F6P + G3P)
0
mmol/g*h
Exch. (MAL OAA)
0.01
mmol/g*h
Exch. (PEP + CO2 OAA)
0.0039932
mmol/g*h
Glucose consumption rate
1.338092110669479
mmol/g*h
Oxygen uptake rate (OUR)
2.1996726677577847
mmol/g*h
Carbon dioxide evolution rate (CER)
0.3142389525368264
mmol/g*h
Ethanol production rate
0.0
mmol/g*h
Acetate production rate
0.000288715630037671
mmol/g*h
D-Lactate production rate
0.0
mmol/g*h
Succinate production rate
0.00041086598166849124
mmol/g*h
Pyruvate production rate
7.758740528989164e-05
mmol/g*h
Formate production rate
0.0
mmol/g*h
Graph Loading...
Reaction
KEGG ID
Flux
Unit
Glucose + PEP -> G6P + PYR
R02738 5.05
mmol/g*h
G6P F6P
R02740 3.14
mmol/g*h
F6P -> F1,6P
R04779 4.05
mmol/g*h
F1,6P -> DHAP + G3P
R01070 4.05
mmol/g*h
DHAP -> G3P
R01015 4.05
mmol/g*h
G3P -> 3PG
R01061 8.44
mmol/g*h
3PG PEP
7.92
mmol/g*h
PEP -> PYR
R00200 2.67
mmol/g*h
PYR -> AcCoA + CO2
R00209 6.87
mmol/g*h
G6P -> 6PG
1.83
mmol/g*h
6PG -> Ru5P + CO2
R01528 1.83
mmol/g*h
Ru5P -> X5P
R01529 0.94
mmol/g*h
Ru5P -> R5P
R01056 0.89
mmol/g*h
R5P + X5P S7P + G3P
R01641 0.55
mmol/g*h
S7P + G3P E4P + F6P
R01827 0.55
mmol/g*h
X5P + E4P F6P + G3P
R01830 0.4
mmol/g*h
AcCoA + OAA -> CIT
R00351 4.15
mmol/g*h
CIT -> ICT
R01324 4.15
mmol/g*h
ICT -> 2-KG + CO2
R00267 2.85
mmol/g*h
2-KG -> SUC + CO2
R08549 2.43
mmol/g*h
SUC -> FUM
R00412 3.73
mmol/g*h
FUM -> MAL
R01082 3.73
mmol/g*h
MAL OAA
R00342 4.82
mmol/g*h
PEP + CO2 OAA
R00341 0.01
mmol/g*h
MAL -> PYR + CO2
R00214 0.2
mmol/g*h
ICT -> Glyoxylate + SUC
R00479 1.3
mmol/g*h
Glyoxylate + AcCoA -> MAL
R00472 1.3
mmol/g*h
6-PG -> G3P + PYR
R01064 mmol/g*h
AcCoA -> Acetate
R00227 0
mmol/g*h
PYR -> Lactate
R00704 0
mmol/g*h
AcCoA -> Ethanol
0
mmol/g*h
G6P -> (Cell synthesis)
0.08
mmol/g*h
F6P -> (Cell synthesis)
0.04
mmol/g*h
R5P -> (Cell synthesis)
0.34
mmol/g*h
E4P -> (Cell synthesis)
0.15
mmol/g*h
G3P -> (Cell synthesis)
0.06
mmol/g*h
3PG -> (Cell synthesis)
0.52
mmol/g*h
PEP -> (Cell synthesis)
0.19
mmol/g*h
PYR -> (Cell synthesis)
1.06
mmol/g*h
AcCoA -> (Cell synthesis)
1.42
mmol/g*h
OAA -> (Cell synthesis)
0.69
mmol/g*h
2KG -> (Cell synthesis)
0.42
mmol/g*h
CO2 -> (Evolution)
14.18
mmol/g*h
Exch. (G6P F6P)
0.05
mmol/g*h
Exch. (3PG PEP)
0.05
mmol/g*h
Exch. (R5P + X5P S7P + G3P)
0
mmol/g*h
Exch. (S7P + G3P E4P + F6P)
0.03
mmol/g*h
Exch. (X5P + E4P F6P + G3P)
0
mmol/g*h
Exch. (MAL OAA)
0.05
mmol/g*h
Exch. (PEP + CO2 OAA)
0.015049
mmol/g*h
Glucose consumption rate
5.049447704671668
mmol/g*h
Oxygen uptake rate (OUR)
9.56904133685144
mmol/g*h
Carbon dioxide evolution rate (CER)
7.88038698328942
mmol/g*h
Ethanol production rate
0.0018203891863534688
mmol/g*h
Acetate production rate
0.005509023555814386
mmol/g*h
D-Lactate production rate
0.0
mmol/g*h
Succinate production rate
0.0036581426638014817
mmol/g*h
Pyruvate production rate
0.0004959569153356474
mmol/g*h
Formate production rate
0.0029737404196507364
mmol/g*h
Graph Loading...
Reaction
KEGG ID
Flux
Unit
Glucose + PEP -> G6P + PYR
R02738 6.63
mmol/g*h
G6P F6P
R02740 5.53
mmol/g*h
F6P -> F1,6P
R04779 5.75
mmol/g*h
F1,6P -> DHAP + G3P
R01070 5.75
mmol/g*h
DHAP -> G3P
R01015 5.75
mmol/g*h
G3P -> 3PG
R01061 11.45
mmol/g*h
3PG PEP
10.72
mmol/g*h
PEP -> PYR
R00200 2.27
mmol/g*h
PYR -> AcCoA + CO2
R00209 7.4
mmol/g*h
G6P -> 6PG
1
mmol/g*h
6PG -> Ru5P + CO2
R01528 1
mmol/g*h
Ru5P -> X5P
R01529 0.28
mmol/g*h
Ru5P -> R5P
R01056 0.72
mmol/g*h
R5P + X5P S7P + G3P
R01641 0.24
mmol/g*h
S7P + G3P E4P + F6P
R01827 0.24
mmol/g*h
X5P + E4P F6P + G3P
R01830 0.03
mmol/g*h
AcCoA + OAA -> CIT
R00351 4.76
mmol/g*h
CIT -> ICT
R01324 4.76
mmol/g*h
ICT -> 2-KG + CO2
R00267 4.76
mmol/g*h
2-KG -> SUC + CO2
R08549 4.17
mmol/g*h
SUC -> FUM
R00412 4.17
mmol/g*h
FUM -> MAL
R01082 4.17
mmol/g*h
MAL OAA
R00342 4.17
mmol/g*h
PEP + CO2 OAA
R00341 1.56
mmol/g*h
MAL -> PYR + CO2
R00214 0
mmol/g*h
ICT -> Glyoxylate + SUC
R00479 mmol/g*h
Glyoxylate + AcCoA -> MAL
R00472 mmol/g*h
6-PG -> G3P + PYR
R01064 mmol/g*h
AcCoA -> Acetate
R00227 0.49
mmol/g*h
PYR -> Lactate
R00704 0
mmol/g*h
AcCoA -> Ethanol
0.15
mmol/g*h
G6P -> (Cell synthesis)
0.11
mmol/g*h
F6P -> (Cell synthesis)
0.05
mmol/g*h
R5P -> (Cell synthesis)
0.48
mmol/g*h
E4P -> (Cell synthesis)
0.21
mmol/g*h
G3P -> (Cell synthesis)
0.08
mmol/g*h
3PG -> (Cell synthesis)
0.73
mmol/g*h
PEP -> (Cell synthesis)
0.27
mmol/g*h
PYR -> (Cell synthesis)
1.5
mmol/g*h
AcCoA -> (Cell synthesis)
2
mmol/g*h
OAA -> (Cell synthesis)
0.97
mmol/g*h
2KG -> (Cell synthesis)
0.59
mmol/g*h
CO2 -> (Evolution)
15.77
mmol/g*h
Exch. (G6P F6P)
0.06
mmol/g*h
Exch. (3PG PEP)
0
mmol/g*h
Exch. (R5P + X5P S7P + G3P)
0
mmol/g*h
Exch. (S7P + G3P E4P + F6P)
0.04
mmol/g*h
Exch. (X5P + E4P F6P + G3P)
0
mmol/g*h
Exch. (MAL OAA)
0.06
mmol/g*h
Exch. (PEP + CO2 OAA)
0.0068952
mmol/g*h
Glucose consumption rate
6.632329678611488
mmol/g*h
Oxygen uptake rate (OUR)
13.052245646196251
mmol/g*h
Carbon dioxide evolution rate (CER)
8.945921173235632
mmol/g*h
Ethanol production rate
0.14644791322016956
mmol/g*h
Acetate production rate
0.4903045675374672
mmol/g*h
D-Lactate production rate
0.0
mmol/g*h
Succinate production rate
0.0
mmol/g*h
Pyruvate production rate
0.0010955591769246649
mmol/g*h
Formate production rate
0.12614458848006146
mmol/g*h
Graph Loading...
Reaction
KEGG ID
Flux
Unit
Glucose + PEP -> G6P + PYR
R02738 13.34
mmol/g*h
G6P F6P
R02740 7.522954499942079
mmol/g*h
F6P -> F1,6P
R04779 10.663721970712663
mmol/g*h
F1,6P -> DHAP + G3P
R01070 10.663721970712663
mmol/g*h
DHAP -> G3P
R01015 10.663721970712663
mmol/g*h
G3P -> 3PG
R01061 22.665711834169567
mmol/g*h
3PG PEP
21.626526538667413
mmol/g*h
PEP -> PYR
R00200 5.67643295175968
mmol/g*h
PYR -> AcCoA + CO2
R00209 16.87615575909977
mmol/g*h
G6P -> 6PG
5.6636265604415525
mmol/g*h
6PG -> Ru5P + CO2
R01528 5.6636265604415525
mmol/g*h
Ru5P -> X5P
R01529 3.215471420126323
mmol/g*h
Ru5P -> R5P
R01056 2.448155140315229
mmol/g*h
R5P + X5P S7P + G3P
R01641 1.7611456060615547
mmol/g*h
S7P + G3P E4P + F6P
R01827 1.7611456060615547
mmol/g*h
X5P + E4P F6P + G3P
R01830 1.4543258140647681
mmol/g*h
AcCoA + OAA -> CIT
R00351 11.925685115186402
mmol/g*h
CIT -> ICT
R01324 11.925685115186402
mmol/g*h
ICT -> 2-KG + CO2
R00267 11.925685115186402
mmol/g*h
2-KG -> SUC + CO2
R08549 11.08126368764742
mmol/g*h
SUC -> FUM
R00412 11.08126368764742
mmol/g*h
FUM -> MAL
R01082 11.08126368764742
mmol/g*h
MAL OAA
R00342 11.076461290903122
mmol/g*h
PEP + CO2 OAA
R00341 2.2339148855557336
mmol/g*h
MAL -> PYR + CO2
R00214 0.004802396744297529
mmol/g*h
ICT -> Glyoxylate + SUC
R00479 #VALUE!
mmol/g*h
Glyoxylate + AcCoA -> MAL
R00472 #VALUE!
mmol/g*h
6-PG -> G3P + PYR
R01064 #VALUE!
mmol/g*h
AcCoA -> Acetate
R00227 1.9329646895797552
mmol/g*h
PYR -> Lactate
R00704 0.005335996382552811
mmol/g*h
AcCoA -> Ethanol
0.15340989599839328
mmol/g*h
G6P -> (Cell synthesis)
0.15340989599839328
mmol/g*h
F6P -> (Cell synthesis)
0.07470394935573935
mmol/g*h
R5P -> (Cell synthesis)
0.6870095342536744
mmol/g*h
E4P -> (Cell synthesis)
0.30681979199678655
mmol/g*h
G3P -> (Cell synthesis)
0.11605792132052362
mmol/g*h
3PG -> (Cell synthesis)
1.0391852955021597
mmol/g*h
PEP -> (Cell synthesis)
0.3761877449699731
mmol/g*h
PYR -> (Cell synthesis)
2.139734549403677
mmol/g*h
AcCoA -> (Cell synthesis)
2.864096058335221
mmol/g*h
OAA -> (Cell synthesis)
1.3846910612724543
mmol/g*h
2KG -> (Cell synthesis)
0.8444214275389823
mmol/g*h
CO2 -> (Evolution)
43.31761863356372
mmol/g*h
0.0
Exch. (G6P F6P)
0.12672991408562922
mmol/g*h
Exch. (3PG PEP)
0.12672991408562922
mmol/g*h
Exch. (R5P + X5P S7P + G3P)
0.0
mmol/g*h
Exch. (S7P + G3P E4P + F6P)
0.04522256934213507
mmol/g*h
Exch. (X5P + E4P F6P + G3P)
0.0
mmol/g*h
Exch. (MAL OAA)
0.12539591498999103
mmol/g*h
Exch. (PEP + CO2 OAA)
0.030148379561423376
mmol/g*h
Glucose consumption rate
13.343821042281059
mmol/g*h
Oxygen uptake rate (OUR)
14.867256637167968
mmol/g*h
Carbon dioxide evolution rate (CER)
10.831858407079519
mmol/g*h
Ethanol production rate
0.1536616652996288
mmol/g*h
Acetate production rate
1.9333875086608887
mmol/g*h
D-Lactate production rate
0.004140806293018628
mmol/g*h
Succinate production rate
0.0
mmol/g*h
Pyruvate production rate
0.005586017042550038
mmol/g*h
Formate production rate
0.0797738446411004
mmol/g*h
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KIMODATAID35_v3.xlsx
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