Repository » Data AccessID 51

Detail View - Data AccessID 51

Backimage Back

General Information info

Manuscript title Dynamic simulation of an in vitro multi-enzyme system.
PubMed ID 17239859
Journal FEBS Letters
Year 2007
Authors Nobuyoshi Ishii, Yoshihiro Suga, Akiko Hagiya, Hisami Watanabe, Hirotada Mori, Masataka Yoshino, Masaru Tomita
Affiliations Institute for Advanced Biosciences, Keio University, Japan
Keywords dynamic simulation, enzyme kinetics, metabolism, glycolysis, in vitro
Full text article Downloadarticle Ishii_2007.pdf
Project name not specified

Experiment Description info

Organism Escherichia coli
Strain K-12 AG1
Data type time-series data of metabolites
Data units mM
Execution date not specified

Experimental Details info

Temperature (0C) 37.0
pH not specified
Carbon source glucose,
Culture mode batch
Process condition aerobic
Dilution rate (h-1)
Working volume (L) 0.2
Biomass concentration (g/L) not specified
Medium composition

LB medium supplemented with 50 µg/ml chloramphenicol to OD600=0.5

General protocol information Sampling method: Samples were obtained 2 h after addition of 0.1 mM isopropyl-b-D-thiogalactopyranoside (IPTG)

Quenching procedure: cells were collected by centrifugation (6000 r.p.m., 5 min at 4 ºC) and resuspended in 10 ml of cold buffer I [50 mM sodium phosphate (pH 7.0), 200 mM NaCl, Protease inhibitor (Complete Mini EDTA-Free, Roche Diagnostics, Basel, Switzerland)].

Extraction technique: sonication

Sample analyzing method: enzymatic

Methods description - Notes

Measurement of metabolite concentrations - A sample was added to 100 mM MOPS buffer (pH 7.2) containing 0.1 mM NADH, 20 mM MgCl2, 5 mM ATP, and 2 mM DTT (I), and maintained at 37 ºC. Then, 1.8 U/ml ALD, 31 U/ml G3PDH, and 90 U/ml TIM (II), 3 U/ml PfkA (III), 18 U/ml Pgi (IV), and 10 U/ml Glk (V) were added sequentially, and A340 was recorded at each step. Concentrations of fructose-1,6-diphosphate (FDP), F6P, G6P, and glucose were calculated from the difference in A340 between I and II, II and III, III and IV, and IV and V, respectively. The FDP concentration of each sample was calibrated by the measured value of a known concentration standard solution of FDP treated using the same procedure employed for sample preparation.

Data file
Downloadmetabolites KIMODATAID51_v0.xlsx
Alternative format(s)
KIMODATAID51_metab_timeseries.csv
Cite|Share

Organism


Related Data: AccessID 30 | AccessID 35 | AccessID 41 | AccessID 44 | AccessID 54 | AccessID 63 | AccessID 64 | AccessID 65 | AccessID 67 | AccessID 68 | AccessID 74 | AccessID 75 | AccessID 78 | AccessID 79 | AccessID 80 | AccessID 86 | AccessID 87 | AccessID 92 | AccessID 96 | AccessID 101 | AccessID 102 | AccessID 103 | AccessID 104 | AccessID 105 | AccessID 106 | AccessID 107 | AccessID 108 | AccessID 109 | AccessID 110 | AccessID 112 | AccessID 116 | AccessID 118 | AccessID 119 | AccessID 125 | AccessID 126


Submission and curation info

Entered by Administrator KiMoSysFirst name: Administrator
Affiliation: INESC-ID/IST
Homepage: http://kdbio.inesc-id.pt/kimosys
Interests: mathematical modeling, accessible data, use of data

Created 2013-05-13 14:45:37 UTC

Updated 2014-05-02 22:19:44 UTC

Version 0

Status (reviewed) 2013-12-06 17:17:38 UTC




Associated Models


Here we can find relevant models associated with Data EntryID 51:

Model
EntryID
Model name Category Model Type Data used for Access Json



Associate models to data

- Several models can be associated.

Add New Model



Backimage Back | Top