Repository » Data AccessID 109

Detail View - Data AccessID 109

Backimage Back

General Information info

Manuscript title Metabolic flux analysis for a ppc mutant Escherichia coli based on 13C-labelling experiments together with enzyme activity assays and intracellular metabolite measurements.
PubMed ID 15158257
Journal FEMS Microbiology Letters
Year 2004
Authors Lifeng Peng, Marcos J. Arauzo-Bravo, Kazuyuki Shimizu
Affiliations Department of Biochemical Engineering and Science, Kyushu Institute of Technology, Iizuka, Fukuoka 820-8502, Japan.
Keywords ppc mutant Escherichia coli, 2-Dimensional nuclear magnetic resonance, Gas chromatography-mass spectrometry, Metabolic flux analysis
Full text article Downloadarticle Peng_2004.pdf
Project name not specified

Experiment Description info

Organism Escherichia coli
Strain BW25113 and ppc mutant
Data type enzyme/protein concentrations
Data units µmol/min mg(protein)
Execution date not specified

Experimental Details info

Temperature (0C) 37
pH 7.0
Carbon source glucose,
Culture mode chemostat
Process condition aerobic
Dilution rate (h-1) 0.2
Working volume (L) 1.0
Biomass concentration (g/L) Yield of biomass on glucose (g/g): 0,48 (WT) and 0,55 (ppc)
Medium composition

M9 minimal medium as described previously [1].

General protocol information Measurement method: 2D gel-MALDI-TOF

Methods description - Notes

Enzyme activities were measured spectrophotometrical. Further details on preparation of cell-free extracts and assay procedures for enzyme activities can been seen in [2].
------------------------------------References----------------------------------
[1] Zhao, J. and Shimizu, K. (2002). J. Biotechnol. 101, 101–117. http://doi.org/cdjhrn
[2] Peng, L. and Shimizu, K. (2003) . Appl. Microbiol. Biotechnol. 61, 163–178. http://doi.org/dt3mcm

Data file
Downloadproteomic KIMODATAID109_v2.xlsx
Alternative format(s)
no file uploaded
Cite|Share

Organism


Related Data: AccessID 30 | AccessID 35 | AccessID 41 | AccessID 44 | AccessID 51 | AccessID 54 | AccessID 63 | AccessID 64 | AccessID 65 | AccessID 67 | AccessID 68 | AccessID 74 | AccessID 75 | AccessID 78 | AccessID 79 | AccessID 80 | AccessID 86 | AccessID 87 | AccessID 92 | AccessID 96 | AccessID 101 | AccessID 102 | AccessID 103 | AccessID 104 | AccessID 105 | AccessID 106 | AccessID 107 | AccessID 108 | AccessID 110 | AccessID 112 | AccessID 116 | AccessID 118 | AccessID 119 | AccessID 125 | AccessID 126


Submission and curation info

Entered by Administrator KiMoSysFirst name: Administrator
Affiliation: INESC-ID/IST
Homepage: http://kdbio.inesc-id.pt/kimosys
Interests: mathematical modeling, accessible data, use of data

Created 2018-07-28 16:46:57 UTC

Updated 2018-07-29 10:36:34 UTC

Version 2

Status (reviewed) 2018-07-28 16:47:40 UTC




Associated Models


Here we can find relevant models associated with Data EntryID 109:

Model
EntryID
Model name Category Model Type Data used for Access Json



Associate models to data

- Several models can be associated.

Add New Model



Backimage Back | Top