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General Information
Manuscript title Metabolic flux analysis for a ppc mutant Escherichia coli based on 13C-labelling experiments together with enzyme activity assays and intracellular metabolite measurements.
PubMed ID 15158257
Journal FEMS Microbiology Letters
Year 2004
Authors Lifeng Peng, Marcos J. Arauzo-Bravo, Kazuyuki Shimizu
Affiliations Department of Biochemical Engineering and Science, Kyushu Institute of Technology, Iizuka, Fukuoka 820-8502, Japan.
Keywords ppc mutant Escherichia coli, 2-Dimensional nuclear magnetic resonance, Gas chromatography-mass spectrometry, Metabolic flux analysis
Full text article Peng_2004.pdf
Project name not specified


Experiment Description
Organism Escherichia coli
Strain BW25113 and ppc mutant
Data type enzyme/protein concentrations
Data units µmol/min mg(protein)
Execution date not specified


Experimental Details
Temperature (°C) 37
pH 7.0
Carbon source glucose,
Culture mode chemostat
Process condition aerobic
Dilution rate (h⁻¹) 0.2
Working volume (L) 1.0
Biomass concentration (g/L) Yield of biomass on glucose (g/g): 0,48 (WT) and 0,55 (ppc)
Medium composition

M9 minimal medium as described previously [1].

General protocol information Measurement method: 2D gel-MALDI-TOF

Methods description - Notes

Enzyme activities were measured spectrophotometrical. Further details on preparation of cell-free extracts and assay procedures for enzyme activities can been seen in [2].
------------------------------------References----------------------------------
[1] Zhao, ...

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Submission and curation

Entered by: Administrator KiMoSysFirst name: Administrator
Affiliation: INESC-ID/IST
Homepage: http://kdbio.inesc-id.pt/kimosys
Interests: mathematical modeling, accessible data, use of data

Created: 2018-07-28 16:46:57 UTC

Updated: 2020-04-24 16:10:36 UTC

Version: 2

Status: (reviewed) 2018-07-28 16:47:40 UTC

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