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General Information
Manuscript title Sampling of intracellular metabolites for stationary and non-stationary 13C metabolic flux analysis in Escherichia coli
PubMed ID 25102204
Journal Analytical Biochemistry
Year 2014
Authors Pierre Millard, Stéphane Massou, Christoph wittmann, Jean-Charles Portais, Fabien Létisse
Affiliations Université de Toulouse, INSA, UPS, INP, LISBP, F-31077 Toulouse, France; INRA, UMR 792, Ingénierie des Systèmes Biologiques et des Procédés, F-31400 Toulouse, France; CNRS, UMR 5504, F-31400 Toulouse, France
Keywords Quantitative metabolomics, Metabolism, Sampling procedures
Full text article Millard_2014.pdf
Project name not specified


Experiment Description
Organism Escherichia coli
Strain K-12 MG1655
Data type metabolites at steady-state
Data units mM
Execution date not specified


Experimental Details
Temperature (°C) 37.0
pH 7.0
Carbon source glucose,
Culture mode batch
Process condition aerobic
Dilution rate (h⁻¹)
Working volume (L) 0.150
Biomass concentration (g/L)
Medium composition

Minimal synthetic medium: 5 mM KH2PO4, 10 mM Na2HPO4, 9 mM NaCl, 40 mM NH4Cl, 0.8 mM MgSO4, 0.1 mM CaCl2, 0.1 g/L thiamine, and 3 g/L glucose. Glucose and thiamine were sterilized by filtration (Minisart polyamide 0.2 μm, Sartorius, Göttingen, Germany), and other solutions were autoclaved separately.

General protocol information Sampling method: different methods (see Figure 1).

Quenching procedure: different methods (see Figure 1).

Extraction technique: hot ethanol

Sample analyzing method: NMR, GC-MS, LC-MS

Methods description - Notes

Samples were collected using the differential method described in detail in [1]. Briefly, 120 μl of broth or filtered extracellular medium (Sartolon polyamide 0.2 μm, Sartorius) was plunged with 120 μl of fully 13C-labeled cellular extract (used as internal standard) in 5 ml ...

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Submission and curation

Entered by: Administrator KiMoSysFirst name: Administrator
Affiliation: INESC-ID/IST
Homepage: http://kdbio.inesc-id.pt/kimosys
Interests: mathematical modeling, accessible data, use of data

Created: 2015-05-01 11:55:03 UTC

Updated: 2020-04-24 16:10:35 UTC

Version: 0

Status: (reviewed) 2015-05-01 12:03:29 UTC

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