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General Information
Manuscript title Latent Pathway Activation and Increased Pathway Capacity Enable Escherichia coli Adaptation to Loss of Key Metabolic Enzymes.
PubMed ID 16319065
Journal Journal of Biological Chemistry
Year 2006
Authors Stephen S. Fong, Annik Nanchen, Bernhard O. Palsson and Uwe Sauer
Affiliations Institute of Molecular Systems Biology, ETH Zurich, Zurich CH-8093, Switzerland
Keywords Escherichia coli, latent pathway activation
Full text article Fong_2006.pdf
Project name not specified


Experiment Description
Organism Escherichia coli
Strain MG1655 and (pgi, ppc, pta, tpi) mutants
Data type flux measurements
Data units mmol/gdw h
Execution date not specified


Experimental Details
Temperature (°C) 37
pH not specified
Carbon source glucose,
Culture mode batch
Process condition aerobic
Dilution rate (h⁻¹)
Working volume (L) 0.03
Biomass concentration (g/L) see spreadsheet
Medium composition

M9 minimal medium supplemented with 2 g/liter of glucose in 500-ml Erlenmeyer flasks. M9 medium contained (per liter of deionized water) 0.8 g of NH4Cl, 0.5 g of NaCl, 7.5 g of Na2HPO4·2H2O, and 3.0 g of KH2PO4. The following components were sterilized separately and then added (per liter final volume of medium): 2 ml of 1 m MgSO4, 1 ml of 0.1 m CaCl2, 0.3 ml of 1 mm filter-sterilized thiamine HCl, and 10 ml of a trace element solution containing (per liter) 1 g of FeCl3·6H2O, 0.18 g of ZnSO4·7H2O, 0.12 g of CuCl2·2H2O, 0.12 g of MnSO4·H2O, and 0.18 g of CoCl2·6H2O. At the start of evolution, initial precultures of each mutant were grown overnight in LB medium before being transferred to minimal medium for adaptive evolution.

General protocol information Flux analysis method: flux ratio, 13C constrained MFA

Platform: GC-MS

Methods description - Notes

After 8 h of incubation at 37 °C and constant shaking, LB precultures were used to inoculate M9 medium precultures that were grown overnight for inoculation of cultures for physiological or 13C-labeling experiments. For 13C-labeling experiments, glucose was added either enti ...

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Submission and curation

Entered by: Administrator KiMoSysFirst name: Administrator
Affiliation: INESC-ID/IST
Homepage: http://kdbio.inesc-id.pt/kimosys
Interests: mathematical modeling, accessible data, use of data

Created: 2018-08-22 22:25:31 UTC

Updated: 2020-04-24 16:10:37 UTC

Version: 2

Status: (reviewed) 2018-08-22 22:25:40 UTC

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