KiMoSys: a web-based repository of experimental data for Kinetic Models of biological Systems

Metadata
Data EntryID	64
Entered by	Administrator KiMoSys
Date Added	2013-06-12
Version	0
General Information
Manuscript title	Impact of global transcriptional regulation by arcA, arcB, cra, crp, cya, fnr, and mlc on glucose catabolism in Escherichia coli.
PubMed ID	15838044
Journal	Journal of Bacteriology
Year	2005
Authors	Annik Perrenoud and Uwe Sauer
Affiliations	Institute of Biotechnology, ETH, Zurich, Switzerland
Keywords	Escherichia coli, aerobic glucose catabolism
Project name	not specified
Experiment Description
Organism	Escherichia coli
Strain	BW25113 and mutants
Data type	flux measurements
Data units	(mmol/g.h)
Execution date	not specified
Experimental Details
Temperature (0C)	37
pH	not specified
Carbon source	glucose
Culture mode	batch
Process condition	aerobic
Dilution rate (h-1)	—
Working volume (L)	0.030-0.050
Biomass concentration (g/L)	not specified
Medium composition	M9 medium (per liter deionized water): 0.8 g of NH4Cl, 0.5 g of NaCl, 7.5 g of Na2HPO4 · 2H2O, and 3.0 g of KH2PO4. The following components were sterilized separately and then added (per liter [final volume] of medium): 2 ml of 1 M MgSO4, 1 ml of 0.1 M CaCl2, 0.3 ml of 1 mM filter-sterilized thiamine HCl, and 10 ml of a trace element solution containing (per liter) 1 g of FeCl3 · 6H2O, 0.18 g of ZnSO4 · 7H2O, 0.12 g of CuCl2 · 2H2O, 0.12 g of MnSO4 · H2O, and 0.18 g of CoCl2 · 6H2O. Sterilized glucose was added to a final concentration of 2 or 3 g per liter.
General protocol information	Flux analysis method: 13C constrained MFA
	Platform: GC-MS
Methods description - Notes	Metabolic flux ratio analysis by GC-MS - Samples for gas chromatography (GC)-mass spectrometry (MS) analysis were prepared as described previously [1]. Briefly, aliquots of 13C-labeled batch cultures were withdrawn during the mid-exponential growth phase, which was defined as an OD600 of 0.8 to 1.2 for aerobic cultures and an OD600 of 0.4 to 0.7 for anaerobic cultures. Cell pellets were hydrolyzed in 6 M HCl at 105 °C for 24 h in sealed microtubes. The hydrolysates were dried under a stream of air at around 60°C and then derivatized at 85°C in 30 µ l of dimethylformamide (Fluka, Buchs, Switzerland) and 30µl of N-(tert-butyldimethylsilyl)-N-methyl-trifluoroacetamide with 1% (vol/vol) tert-butyldimethylchlorosilane (Fluka) for 60 min. Derivatized amino acids were analyzed with a series 8000 gas chromatograph combined with an MD 800 mass spectrometer (Fisons Instruments, Beverly, Mass.). The GC-MS-derived mass isotope distributions of proteinogenic amino acids were then corrected for naturally occurring isotopes [1]. The corrected mass distributions were related to the in vivo metabolic activities with previously described algebraic equations and statistical data treatment, which quantified nine ratios of fluxes through converging reactions and pathways for the synthesis of five intracellular metabolites [1]. 13C-constrained metabolic net flux analysis - Intracellular net carbon fluxes were estimated by using the previously described [2] stoichiometric model. -----------References------------ [1] Fischer, E., and U. Sauer. 2003. Metabolic flux profiling of Escherichia coli mutants in central carbon metabolism using GC-MS. Eur. J. Biochem. 270: 880–891. [2] Fischer, E., N. Zamboni, and U. Sauer. 2004. High-throughput metabolic flux analysis based on gas chromatography-mass spectrometry derived 13C constraints. Anal. Biochem. 325:308–316.
Data URL	http://kdbio.inesc-id.pt/kimosys/repository/64

Reaction	KEGG ID	Flux	Std	Unit
GLC + ATP -> G6P	R02738	7.7	0.09	mmol/g*h
G6P -> 6PG + NADPH	R01528	2.27	0.1	mmol/g*h
6PG -> P5P + CO2 + NADPH	R10221	1.98	0.11	mmol/g*h
G6P -> F6P	R02740	5.39	0.12	mmol/g*h
6PG -> T3P + PYR	R05605	0.29	0.13	mmol/g*h
F6P + ATP -> 2 T3P		6.19	0.14	mmol/g*h
2 P5P -> S7P + T3P		0.54	0.04	mmol/g*h
P5P + E4P -> F6P + T3P	R01067	0.31	0.04	mmol/g*h
S7P + T3P -> E4P + F6P	R01827	0.54	0.04	mmol/g*h
T3P -> PGA + ATP + NADH		12.91	0.22	mmol/g*h
PGA -> PEP		11.78	0.22	mmol/g*h
PEP -> PYR + ATP	R00200	9.21	0.23	mmol/g*h
PYR -> AcCoA + CO2 + NADH	R01196	7.9	0.23	mmol/g*h
OAA + AcCoA -> ICT		1.86	0.24	mmol/g*h
ICT -> OGA + CO2 + NADPH	R00267	1.86	0.24	mmol/g*h
OGA -> FUM + CO2 + 1.5 ATP + 2 NADH		1.06	0.24	mmol/g*h
FUM -> MAL	R01082	1.06	0.24	mmol/g*h
MAL -> OAA + NADH	R00342	0.82	0.18	mmol/g*h
MAL -> PYR + CO2 + NADH	R00214	0.24	0.14	mmol/g*h
OAA + ATP -> PEP + CO2	R00341	0.01	0.02	mmol/g*h
PEP + CO2 -> OAA	R00341	2.09	0.15	mmol/g*h
AcCoA -> Acetate + ATP	R00229	4.59	0.18	mmol/g*h
NADPH -> NADH	R00105	-4.04	0.72	mmol/g*h
O2 + 2 NADH -> 2 PO + ATP		10.93	0.71	mmol/g*h
Biomass		0.64	0.01	mmol/g*h

Graph

Loading...

Reaction	KEGG ID	Flux	Std	Unit
GLC + ATP -> G6P	R02738	7.3	0.2	mmol/g*h
G6P -> 6PG + NADPH	R01528	2	0.1	mmol/g*h
6PG -> P5P + CO2 + NADPH	R10221	1.6	0.1	mmol/g*h
G6P -> F6P	R02740	5.3	0.2	mmol/g*h
6PG -> T3P + PYR	R05605	0.4	0.1	mmol/g*h
F6P + ATP -> 2 T3P		5.8	0.2	mmol/g*h
2 P5P -> S7P + T3P		0.4	0	mmol/g*h
P5P + E4P -> F6P + T3P	R01067	0.2	0	mmol/g*h
S7P + T3P -> E4P + F6P	R01827	0.4	0	mmol/g*h
T3P -> PGA + ATP + NADH		12.2	0.3	mmol/g*h
PGA -> PEP		11.1	0.3	mmol/g*h
PEP -> PYR + ATP	R00200	8.7	0.3	mmol/g*h
PYR -> AcCoA + CO2 + NADH	R01196	7.5	0.3	mmol/g*h
OAA + AcCoA -> ICT		2.6	0.3	mmol/g*h
ICT -> OGA + CO2 + NADPH	R00267	2.6	0.3	mmol/g*h
OGA -> FUM + CO2 + 1.5 ATP + 2 NADH		1.8	0.3	mmol/g*h
FUM -> MAL	R01082	1.8	0.3	mmol/g*h
MAL -> OAA + NADH	R00342	1.6	0.2	mmol/g*h
MAL -> PYR + CO2 + NADH	R00214	0.2	0.1	mmol/g*h
OAA + ATP -> PEP + CO2	R00341	0	0.1	mmol/g*h
PEP + CO2 -> OAA	R00341	2	0.2	mmol/g*h
AcCoA -> Acetate + ATP	R00229	3.5	0.1	mmol/g*h
NADPH -> NADH	R00105	-3.4	0.8	mmol/g*h
O2 + 2 NADH -> 2 PO + ATP		11.8	0.9	mmol/g*h
Biomass		0.6	0.01	mmol/g*h

Graph

Loading...

Reaction	KEGG ID	Flux	Std	Unit
GLC + ATP -> G6P	R02738	7.5	0.1	mmol/g*h
G6P -> 6PG + NADPH	R01528	2.3	0.1	mmol/g*h
6PG -> P5P + CO2 + NADPH	R10221	1.9	0.1	mmol/g*h
G6P -> F6P	R02740	5.1	0.1	mmol/g*h
6PG -> T3P + PYR	R05605	0.5	0.1	mmol/g*h
F6P + ATP -> 2 T3P		5.9	0.1	mmol/g*h
2 P5P -> S7P + T3P		0.5	0	mmol/g*h
P5P + E4P -> F6P + T3P	R01067	0.3	0	mmol/g*h
S7P + T3P -> E4P + F6P	R01827	0.5	0	mmol/g*h
T3P -> PGA + ATP + NADH		12.4	0.2	mmol/g*h
PGA -> PEP		11.4	0.2	mmol/g*h
PEP -> PYR + ATP	R00200	9	0.2	mmol/g*h
PYR -> AcCoA + CO2 + NADH	R01196	7.9	0.2	mmol/g*h
OAA + AcCoA -> ICT		1.6	0.2	mmol/g*h
ICT -> OGA + CO2 + NADPH	R00267	1.6	0.2	mmol/g*h
OGA -> FUM + CO2 + 1.5 ATP + 2 NADH		0.9	0.2	mmol/g*h
FUM -> MAL	R01082	0.9	0.2	mmol/g*h
MAL -> OAA + NADH	R00342	0.7	0.2	mmol/g*h
MAL -> PYR + CO2 + NADH	R00214	0.2	0.1	mmol/g*h
OAA + ATP -> PEP + CO2	R00341	0	0.1	mmol/g*h
PEP + CO2 -> OAA	R00341	1.9	0.2	mmol/g*h
AcCoA -> Acetate + ATP	R00229	4.9	0.1	mmol/g*h
NADPH -> NADH	R00105	-3.8	0.7	mmol/g*h
O2 + 2 NADH -> 2 PO + ATP		10.5	0.7	mmol/g*h
Biomass		0.6	0.01	mmol/g*h

Graph

Loading...

Reaction	KEGG ID	Flux	Std	Unit
GLC + ATP -> G6P	R02738	8.4	0.2	mmol/g*h
G6P -> 6PG + NADPH	R01528	2.4	0.1	mmol/g*h
6PG -> P5P + CO2 + NADPH	R10221	2.1	0.1	mmol/g*h
G6P -> F6P	R02740	6	0.2	mmol/g*h
6PG -> T3P + PYR	R05605	0.3	0.1	mmol/g*h
F6P + ATP -> 2 T3P		6.8	0.2	mmol/g*h
2 P5P -> S7P + T3P		0.6	0	mmol/g*h
P5P + E4P -> F6P + T3P	R01067	0.3	0	mmol/g*h
S7P + T3P -> E4P + F6P	R01827	0.6	0	mmol/g*h
T3P -> PGA + ATP + NADH		14.3	0.4	mmol/g*h
PGA -> PEP		13.1	0.4	mmol/g*h
PEP -> PYR + ATP	R00200	10.2	0.4	mmol/g*h
PYR -> AcCoA + CO2 + NADH	R01196	9.1	0.4	mmol/g*h
OAA + AcCoA -> ICT		2.2	0.3	mmol/g*h
ICT -> OGA + CO2 + NADPH	R00267	2.2	0.3	mmol/g*h
OGA -> FUM + CO2 + 1.5 ATP + 2 NADH		1.4	0.3	mmol/g*h
FUM -> MAL	R01082	1.4	0.3	mmol/g*h
MAL -> OAA + NADH	R00342	0.9	0.2	mmol/g*h
MAL -> PYR + CO2 + NADH	R00214	0.5	0.2	mmol/g*h
OAA + ATP -> PEP + CO2	R00341	0	0	mmol/g*h
PEP + CO2 -> OAA	R00341	2.4	0.2	mmol/g*h
AcCoA -> Acetate + ATP	R00229	5.4	0.3	mmol/g*h
NADPH -> NADH	R00105	-3.5	0.8	mmol/g*h
O2 + 2 NADH -> 2 PO + ATP		13	1	mmol/g*h
Biomass		0.65	0.01	mmol/g*h

Graph

Loading...

Reaction	KEGG ID	Flux	Std	Unit
GLC + ATP -> G6P	R02738	2.6	0.1	mmol/g*h
G6P -> 6PG + NADPH	R01528	0.9	0.1	mmol/g*h
6PG -> P5P + CO2 + NADPH	R10221	0.8	0.1	mmol/g*h
G6P -> F6P	R02740	1.7	0.1	mmol/g*h
6PG -> T3P + PYR	R05605	0.1	0.1	mmol/g*h
F6P + ATP -> 2 T3P		2.1	0.1	mmol/g*h
2 P5P -> S7P + T3P		0.3	0	mmol/g*h
P5P + E4P -> F6P + T3P	R01067	0.2	0	mmol/g*h
S7P + T3P -> E4P + F6P	R01827	0.3	0	mmol/g*h
T3P -> PGA + ATP + NADH		4.4	0.2	mmol/g*h
PGA -> PEP		4	0.2	mmol/g*h
PEP -> PYR + ATP	R00200	3	0.2	mmol/g*h
PYR -> AcCoA + CO2 + NADH	R01196	2.6	0.2	mmol/g*h
OAA + AcCoA -> ICT		1.5	0.2	mmol/g*h
ICT -> OGA + CO2 + NADPH	R00267	1.5	0.2	mmol/g*h
OGA -> FUM + CO2 + 1.5 ATP + 2 NADH		1.2	0.2	mmol/g*h
FUM -> MAL	R01082	1.2	0.2	mmol/g*h
MAL -> OAA + NADH	R00342	1	0.2	mmol/g*h
MAL -> PYR + CO2 + NADH	R00214	0.2	0.1	mmol/g*h
OAA + ATP -> PEP + CO2	R00341	0.1	0.1	mmol/g*h
PEP + CO2 -> OAA	R00341	0.8	0.1	mmol/g*h
AcCoA -> Acetate + ATP	R00229	0.6	0.1	mmol/g*h
NADPH -> NADH	R00105	-0.9	0.7	mmol/g*h
O2 + 2 NADH -> 2 PO + ATP		5.1	0.6	mmol/g*h
Biomass		0.24	0.01	mmol/g*h

Graph

Loading...

Reaction	KEGG ID	Flux	Std	Unit
GLC + ATP -> G6P	R02738	2.8	0.1	mmol/g*h
G6P -> 6PG + NADPH	R01528	1	0.1	mmol/g*h
6PG -> P5P + CO2 + NADPH	R10221	0.9	0.1	mmol/g*h
G6P -> F6P	R02740	1.8	0.1	mmol/g*h
6PG -> T3P + PYR	R05605	0.1	0.1	mmol/g*h
F6P + ATP -> 2 T3P		2.2	0.1	mmol/g*h
2 P5P -> S7P + T3P		0.3	0	mmol/g*h
P5P + E4P -> F6P + T3P	R01067	0.2	0	mmol/g*h
S7P + T3P -> E4P + F6P	R01827	0.3	0	mmol/g*h
T3P -> PGA + ATP + NADH		4.7	0.2	mmol/g*h
PGA -> PEP		4.3	0.2	mmol/g*h
PEP -> PYR + ATP	R00200	3.2	0.2	mmol/g*h
PYR -> AcCoA + CO2 + NADH	R01196	2.7	0.2	mmol/g*h
OAA + AcCoA -> ICT		1.4	0.2	mmol/g*h
ICT -> OGA + CO2 + NADPH	R00267	1.4	0.2	mmol/g*h
OGA -> FUM + CO2 + 1.5 ATP + 2 NADH		1.1	0.2	mmol/g*h
FUM -> MAL	R01082	1.1	0.2	mmol/g*h
MAL -> OAA + NADH	R00342	0.9	0.2	mmol/g*h
MAL -> PYR + CO2 + NADH	R00214	0.2	0.1	mmol/g*h
OAA + ATP -> PEP + CO2	R00341	0.1	0.1	mmol/g*h
PEP + CO2 -> OAA	R00341	1	0.1	mmol/g*h
AcCoA -> Acetate + ATP	R00229	0.7	0.1	mmol/g*h
NADPH -> NADH	R00105	-1.1	0.7	mmol/g*h
O2 + 2 NADH -> 2 PO + ATP		5.1	0.6	mmol/g*h
Biomass		0.26	0.01	mmol/g*h

Graph

Loading...

Reaction	KEGG ID	Flux	Std	Unit
GLC + ATP -> G6P	R02738	7.8	0.2	mmol/g*h
G6P -> 6PG + NADPH	R01528	2.4	0.1	mmol/g*h
6PG -> P5P + CO2 + NADPH	R10221	2	0.1	mmol/g*h
G6P -> F6P	R02740	5.4	0.2	mmol/g*h
6PG -> T3P + PYR	R05605	0.4	0.2	mmol/g*h
F6P + ATP -> 2 T3P		6.2	0.2	mmol/g*h
2 P5P -> S7P + T3P		0.6	0	mmol/g*h
P5P + E4P -> F6P + T3P	R01067	0.3	0	mmol/g*h
S7P + T3P -> E4P + F6P	R01827	0.6	0	mmol/g*h
T3P -> PGA + ATP + NADH		13.1	0.4	mmol/g*h
PGA -> PEP		12	0.4	mmol/g*h
PEP -> PYR + ATP	R00200	9.5	0.3	mmol/g*h
PYR -> AcCoA + CO2 + NADH	R01196	8.2	0.3	mmol/g*h
OAA + AcCoA -> ICT		2	0.3	mmol/g*h
ICT -> OGA + CO2 + NADPH	R00267	2	0.3	mmol/g*h
OGA -> FUM + CO2 + 1.5 ATP + 2 NADH		1.2	0.3	mmol/g*h
FUM -> MAL	R01082	1.2	0.3	mmol/g*h
MAL -> OAA + NADH	R00342	1	0.2	mmol/g*h
MAL -> PYR + CO2 + NADH	R00214	0.2	0.2	mmol/g*h
OAA + ATP -> PEP + CO2	R00341	0.1	0.2	mmol/g*h
PEP + CO2 -> OAA	R00341	2.1	0.2	mmol/g*h
AcCoA -> Acetate + ATP	R00229	4.8	0.1	mmol/g*h
NADPH -> NADH	R00105	-3.3	0.8	mmol/g*h
O2 + 2 NADH -> 2 PO + ATP		11.7	1	mmol/g*h
Biomass		0.61	0.01	mmol/g*h

Graph

Loading...

Reaction	KEGG ID	Flux	Std	Unit
GLC + ATP -> G6P	R02738	7.2	0.1	mmol/g*h
G6P -> 6PG + NADPH	R01528	2.3	0.1	mmol/g*h
6PG -> P5P + CO2 + NADPH	R10221	1.8	0.1	mmol/g*h
G6P -> F6P	R02740	4.9	0.1	mmol/g*h
6PG -> T3P + PYR	R05605	0.5	0.1	mmol/g*h
F6P + ATP -> 2 T3P		5.6	0.1	mmol/g*h
2 P5P -> S7P + T3P		0.5	0	mmol/g*h
P5P + E4P -> F6P + T3P	R01067	0.3	0	mmol/g*h
S7P + T3P -> E4P + F6P	R01827	0.5	0	mmol/g*h
T3P -> PGA + ATP + NADH		11.9	0.2	mmol/g*h
PGA -> PEP		10.9	0.2	mmol/g*h
PEP -> PYR + ATP	R00200	8.4	0.2	mmol/g*h
PYR -> AcCoA + CO2 + NADH	R01196	7.4	0.2	mmol/g*h
OAA + AcCoA -> ICT		1.9	0.2	mmol/g*h
ICT -> OGA + CO2 + NADPH	R00267	1.9	0.2	mmol/g*h
OGA -> FUM + CO2 + 1.5 ATP + 2 NADH		1.2	0.2	mmol/g*h
FUM -> MAL	R01082	1.2	0.2	mmol/g*h
MAL -> OAA + NADH	R00342	0.9	0.2	mmol/g*h
MAL -> PYR + CO2 + NADH	R00214	0.3	0.1	mmol/g*h
OAA + ATP -> PEP + CO2	R00341	0	0	mmol/g*h
PEP + CO2 -> OAA	R00341	2	0.1	mmol/g*h
AcCoA -> Acetate + ATP	R00229	4.1	0.2	mmol/g*h
NADPH -> NADH	R00105	-3.4	0.7	mmol/g*h
O2 + 2 NADH -> 2 PO + ATP		10.6	0.7	mmol/g*h
Biomass		0.59	0.01	mmol/g*h

Graph

Loading...

Reaction	KEGG ID	Flux	Std	Unit
GLC + ATP -> G6P	R02738	11.6	0.1	mmol/g*h
G6P -> 6PG + NADPH	R01528	3.6	0.16	mmol/g*h
6PG -> P5P + CO2 + NADPH	R10221	3	0.18	mmol/g*h
G6P -> F6P	R02740	8	0.17	mmol/g*h
6PG -> T3P + PYR	R05605	0.6	0.22	mmol/g*h
F6P + ATP -> 2 T3P		9.1	0.21	mmol/g*h
2 P5P -> S7P + T3P		0.7	0.06	mmol/g*h
P5P + E4P -> F6P + T3P	R01067	0.4	0.06	mmol/g*h
S7P + T3P -> E4P + F6P	R01827	0.7	0.06	mmol/g*h
T3P -> PGA + ATP + NADH		19.2	0.32	mmol/g*h
PGA -> PEP		17.4	0.4	mmol/g*h
PEP -> PYR + ATP	R00200	13.9	0.34	mmol/g*h
PYR -> AcCoA + CO2 + NADH	R01196	12.5	0.71	mmol/g*h
OAA + AcCoA -> ICT		2.6	0.85	mmol/g*h
ICT -> OGA + CO2 + NADPH	R00267	2.6	0.85	mmol/g*h
OGA -> FUM + CO2 + 1.5 ATP + 2 NADH		1.6	0.92	mmol/g*h
FUM -> MAL	R01082	1.6	0.92	mmol/g*h
MAL -> OAA + NADH	R00342	1.1	0.29	mmol/g*h
MAL -> PYR + CO2 + NADH	R00214	0.4	0.71	mmol/g*h
OAA + ATP -> PEP + CO2	R00341	0.1	0.23	mmol/g*h
PEP + CO2 -> OAA	R00341	3	0.59	mmol/g*h
AcCoA -> Acetate + ATP	R00229	7.8	0.1	mmol/g*h
NADPH -> NADH	R00105	-4.5	1.78	mmol/g*h
O2 + 2 NADH -> 2 PO + ATP		17.4	2.57	mmol/g*h
Biomass		0.91	0.06	mmol/g*h

Graph

Loading...

Reaction	KEGG ID	Flux	Std	Unit
GLC + ATP -> G6P	R02738	9.3	0.1	mmol/g*h
G6P -> 6PG + NADPH	R01528	2.5	0.1	mmol/g*h
6PG -> P5P + CO2 + NADPH	R10221	2.1	0.1	mmol/g*h
G6P -> F6P	R02740	6.8	0.1	mmol/g*h
6PG -> T3P + PYR	R05605	0.4	0.2	mmol/g*h
F6P + ATP -> 2 T3P		7.7	0.2	mmol/g*h
2 P5P -> S7P + T3P		0.6	0	mmol/g*h
P5P + E4P -> F6P + T3P	R01067	0.3	0	mmol/g*h
S7P + T3P -> E4P + F6P	R01827	0.6	0	mmol/g*h
T3P -> PGA + ATP + NADH		16	0.2	mmol/g*h
PGA -> PEP		14.8	0.3	mmol/g*h
PEP -> PYR + ATP	R00200	12.3	0.3	mmol/g*h
PYR -> AcCoA + CO2 + NADH	R01196	10.7	0.2	mmol/g*h
OAA + AcCoA -> ICT		3.7	0.3	mmol/g*h
ICT -> OGA + CO2 + NADPH	R00267	3.7	0.3	mmol/g*h
OGA -> FUM + CO2 + 1.5 ATP + 2 NADH		2.8	0.3	mmol/g*h
FUM -> MAL	R01082	2.8	0.3	mmol/g*h
MAL -> OAA + NADH	R00342	2.8	0.3	mmol/g*h
MAL -> PYR + CO2 + NADH	R00214	0	0.1	mmol/g*h
OAA + ATP -> PEP + CO2	R00341	0.5	0.2	mmol/g*h
PEP + CO2 -> OAA	R00341	2.5	0.2	mmol/g*h
AcCoA -> Acetate + ATP	R00229	5.4	0.1	mmol/g*h
NADPH -> NADH	R00105	-2.2	0.7	mmol/g*h
O2 + 2 NADH -> 2 PO + ATP		17.4	0.8	mmol/g*h
Biomass		0.66	0.01	mmol/g*h

Graph

Loading...

Reaction	KEGG ID	Flux	Std	Unit
GLC + ATP -> G6P	R02738	10.6	0.4	mmol/g*h
G6P -> 6PG + NADPH	R01528	3.6	0.2	mmol/g*h
6PG -> P5P + CO2 + NADPH	R10221	3.1	0.2	mmol/g*h
G6P -> F6P	R02740	7	0.3	mmol/g*h
6PG -> T3P + PYR	R05605	0.5	0.2	mmol/g*h
F6P + ATP -> 2 T3P		8.3	0.4	mmol/g*h
2 P5P -> S7P + T3P		0.8	0.1	mmol/g*h
P5P + E4P -> F6P + T3P	R01067	0.5	0.1	mmol/g*h
S7P + T3P -> E4P + F6P	R01827	0.8	0.1	mmol/g*h
T3P -> PGA + ATP + NADH		17.4	0.8	mmol/g*h
PGA -> PEP		15.8	0.8	mmol/g*h
PEP -> PYR + ATP	R00200	12.3	0.6	mmol/g*h
PYR -> AcCoA + CO2 + NADH	R01196	10.7	0.8	mmol/g*h
OAA + AcCoA -> ICT		3.2	0.8	mmol/g*h
ICT -> OGA + CO2 + NADPH	R00267	3.2	0.8	mmol/g*h
OGA -> FUM + CO2 + 1.5 ATP + 2 NADH		2.2	0.8	mmol/g*h
FUM -> MAL	R01082	2.2	0.8	mmol/g*h
MAL -> OAA + NADH	R00342	1.8	0.4	mmol/g*h
MAL -> PYR + CO2 + NADH	R00214	0.4	0.5	mmol/g*h
OAA + ATP -> PEP + CO2	R00341	0.4	0.2	mmol/g*h
PEP + CO2 -> OAA	R00341	3.3	0.5	mmol/g*h
AcCoA -> Acetate + ATP	R00229	5.5	0.5	mmol/g*h
NADPH -> NADH	R00105	-3.4	1.2	mmol/g*h
O2 + 2 NADH -> 2 PO + ATP		17	2.4	mmol/g*h
Biomass		0.88	0.03	mmol/g*h

Graph

Loading...

Reaction	KEGG ID	Flux	Std	Unit
GLC + ATP -> G6P	R02738	7.2	0.1	mmol/g*h
G6P -> 6PG + NADPH	R01528	2.1	0.1	mmol/g*h
6PG -> P5P + CO2 + NADPH	R10221	1.8	0.1	mmol/g*h
G6P -> F6P	R02740	5.1	0.1	mmol/g*h
6PG -> T3P + PYR	R05605	0.3	0.1	mmol/g*h
F6P + ATP -> 2 T3P		5.9	0.2	mmol/g*h
2 P5P -> S7P + T3P		0.5	0	mmol/g*h
P5P + E4P -> F6P + T3P	R01067	0.3	0	mmol/g*h
S7P + T3P -> E4P + F6P	R01827	0.5	0	mmol/g*h
T3P -> PGA + ATP + NADH		12.3	0.2	mmol/g*h
PGA -> PEP		11.5	0.2	mmol/g*h
PEP -> PYR + ATP	R00200	9.3	0.2	mmol/g*h
PYR -> AcCoA + CO2 + NADH	R01196	8.3	0.2	mmol/g*h
OAA + AcCoA -> ICT		2.9	0.3	mmol/g*h
ICT -> OGA + CO2 + NADPH	R00267	2.9	0.3	mmol/g*h
OGA -> FUM + CO2 + 1.5 ATP + 2 NADH		2.2	0.3	mmol/g*h
FUM -> MAL	R01082	2.2	0.3	mmol/g*h
MAL -> OAA + NADH	R00342	2	0.2	mmol/g*h
MAL -> PYR + CO2 + NADH	R00214	0.2	0.1	mmol/g*h
OAA + ATP -> PEP + CO2	R00341	0.1	0.2	mmol/g*h
PEP + CO2 -> OAA	R00341	1.8	0.2	mmol/g*h
AcCoA -> Acetate + ATP	R00229	4.3	0.1	mmol/g*h
NADPH -> NADH	R00105	-1.4	0.7	mmol/g*h
O2 + 2 NADH -> 2 PO + ATP		13.6	0.8	mmol/g*h
Biomass		0.5	0.01	mmol/g*h

Graph

Loading...

Reaction	KEGG ID	Flux	Std	Unit
GLC + ATP -> G6P	R02738	8.3	0.3	mmol/g*h
G6P -> 6PG + NADPH	R01528	2.2	0.1	mmol/g*h
6PG -> P5P + CO2 + NADPH	R10221	1.9	0.1	mmol/g*h
G6P -> F6P	R02740	6.1	0.2	mmol/g*h
6PG -> T3P + PYR	R05605	0.3	0.2	mmol/g*h
F6P + ATP -> 2 T3P		7	0.3	mmol/g*h
2 P5P -> S7P + T3P		0.6	0	mmol/g*h
P5P + E4P -> F6P + T3P	R01067	0.4	0	mmol/g*h
S7P + T3P -> E4P + F6P	R01827	0.6	0	mmol/g*h
T3P -> PGA + ATP + NADH		14.5	0.5	mmol/g*h
PGA -> PEP		13.7	0.5	mmol/g*h
PEP -> PYR + ATP	R00200	11.4	0.5	mmol/g*h
PYR -> AcCoA + CO2 + NADH	R01196	10.3	0.5	mmol/g*h
OAA + AcCoA -> ICT		1.9	0.3	mmol/g*h
ICT -> OGA + CO2 + NADPH	R00267	1.9	0.3	mmol/g*h
OGA -> FUM + CO2 + 1.5 ATP + 2 NADH		1.2	0.3	mmol/g*h
FUM -> MAL	R01082	1.2	0.3	mmol/g*h
MAL -> OAA + NADH	R00342	0.9	0.2	mmol/g*h
MAL -> PYR + CO2 + NADH	R00214	0.3	0.2	mmol/g*h
OAA + ATP -> PEP + CO2	R00341	0	0.1	mmol/g*h
PEP + CO2 -> OAA	R00341	1.8	0.2	mmol/g*h
AcCoA -> Acetate + ATP	R00229	7.3	0.4	mmol/g*h
NADPH -> NADH	R00105	-2.3	0.8	mmol/g*h
O2 + 2 NADH -> 2 PO + ATP		13.7	1.1	mmol/g*h
Biomass		0.51	0.01	mmol/g*h

Graph

Loading...

Reaction	KEGG ID	Flux	Std	Unit
GLC + ATP -> G6P	R02738	2.9	0.1	mmol/g*h
G6P -> 6PG + NADPH	R01528	0.7	0	mmol/g*h
6PG -> P5P + CO2 + NADPH	R10221	0.6	0.1	mmol/g*h
G6P -> F6P	R02740	2.1	0.1	mmol/g*h
6PG -> T3P + PYR	R05605	0.1	0.1	mmol/g*h
F6P + ATP -> 2 T3P		2.4	0.1	mmol/g*h
2 P5P -> S7P + T3P		0.2	0	mmol/g*h
P5P + E4P -> F6P + T3P	R01067	0.1	0	mmol/g*h
S7P + T3P -> E4P + F6P	R01827	0.2	0	mmol/g*h
T3P -> PGA + ATP + NADH		5	0.2	mmol/g*h
PGA -> PEP		4.7	0.2	mmol/g*h
PEP -> PYR + ATP	R00200	3.7	0.2	mmol/g*h
PYR -> AcCoA + CO2 + NADH	R01196	3.4	0.2	mmol/g*h
OAA + AcCoA -> ICT		1.1	0.2	mmol/g*h
ICT -> OGA + CO2 + NADPH	R00267	1.1	0.2	mmol/g*h
OGA -> FUM + CO2 + 1.5 ATP + 2 NADH		0.8	0.2	mmol/g*h
FUM -> MAL	R01082	0.8	0.2	mmol/g*h
MAL -> OAA + NADH	R00342	0.7	0.1	mmol/g*h
MAL -> PYR + CO2 + NADH	R00214	0.2	0.1	mmol/g*h
OAA + ATP -> PEP + CO2	R00341	0	0.1	mmol/g*h
PEP + CO2 -> OAA	R00341	0.7	0.1	mmol/g*h
AcCoA -> Acetate + ATP	R00229	1.9	0.1	mmol/g*h
NADPH -> NADH	R00105	-1.5	0.7	mmol/g*h
O2 + 2 NADH -> 2 PO + ATP		5	0.6	mmol/g*h
Biomass		0.23	0.01	mmol/g*h

Graph

Loading...

Reaction	KEGG ID	Flux	Std	Unit
GLC + ATP -> G6P	R02738	8.3	0.3	mmol/g*h
G6P -> 6PG + NADPH	R01528	2.4	0.1	mmol/g*h
6PG -> P5P + CO2 + NADPH	R10221	1.9	0.1	mmol/g*h
G6P -> F6P	R02740	5.9	0.2	mmol/g*h
6PG -> T3P + PYR	R05605	0.5	0.2	mmol/g*h
F6P + ATP -> 2 T3P		6.6	0.3	mmol/g*h
2 P5P -> S7P + T3P		0.5	0	mmol/g*h
P5P + E4P -> F6P + T3P	R01067	0.3	0	mmol/g*h
S7P + T3P -> E4P + F6P	R01827	0.5	0	mmol/g*h
T3P -> PGA + ATP + NADH		13.9	0.5	mmol/g*h
PGA -> PEP		12.7	0.5	mmol/g*h
PEP -> PYR + ATP	R00200	10.1	0.5	mmol/g*h
PYR -> AcCoA + CO2 + NADH	R01196	8.7	0.5	mmol/g*h
OAA + AcCoA -> ICT		2.2	0.3	mmol/g*h
ICT -> OGA + CO2 + NADPH	R00267	2.2	0.3	mmol/g*h
OGA -> FUM + CO2 + 1.5 ATP + 2 NADH		1.3	0.3	mmol/g*h
FUM -> MAL	R01082	1.3	0.3	mmol/g*h
MAL -> OAA + NADH	R00342	1.3	0.3	mmol/g*h
MAL -> PYR + CO2 + NADH	R00214	0	0.1	mmol/g*h
OAA + ATP -> PEP + CO2	R00341	0.2	0.3	mmol/g*h
PEP + CO2 -> OAA	R00341	2.2	0.4	mmol/g*h
AcCoA -> Acetate + ATP	R00229	4.9	0.5	mmol/g*h
NADPH -> NADH	R00105	-3.9	0.8	mmol/g*h
O2 + 2 NADH -> 2 PO + ATP		12.3	1	mmol/g*h
Biomass		0.66	0.01	mmol/g*h

Graph

Loading...

Reaction	KEGG ID	Flux	Std	Unit
GLC + ATP -> G6P	R02738	8	0.2	mmol/g*h
G6P -> 6PG + NADPH	R01528	2.2	0.1	mmol/g*h
6PG -> P5P + CO2 + NADPH	R10221	1.8	0.1	mmol/g*h
G6P -> F6P	R02740	5.8	0.2	mmol/g*h
6PG -> T3P + PYR	R05605	0.4	0.1	mmol/g*h
F6P + ATP -> 2 T3P		6.4	0.2	mmol/g*h
2 P5P -> S7P + T3P		0.5	0	mmol/g*h
P5P + E4P -> F6P + T3P	R01067	0.2	0	mmol/g*h
S7P + T3P -> E4P + F6P	R01827	0.5	0	mmol/g*h
T3P -> PGA + ATP + NADH		13.4	0.4	mmol/g*h
PGA -> PEP		12.1	0.4	mmol/g*h
PEP -> PYR + ATP	R00200	9.6	0.4	mmol/g*h
PYR -> AcCoA + CO2 + NADH	R01196	8	0.4	mmol/g*h
OAA + AcCoA -> ICT		2.3	0.3	mmol/g*h
ICT -> OGA + CO2 + NADPH	R00267	2.3	0.3	mmol/g*h
OGA -> FUM + CO2 + 1.5 ATP + 2 NADH		1.4	0.3	mmol/g*h
FUM -> MAL	R01082	1.4	0.3	mmol/g*h
MAL -> OAA + NADH	R00342	1.4	0.2	mmol/g*h
MAL -> PYR + CO2 + NADH	R00214	0	0.2	mmol/g*h
OAA + ATP -> PEP + CO2	R00341	0.3	0.2	mmol/g*h
PEP + CO2 -> OAA	R00341	2.4	0.2	mmol/g*h
AcCoA -> Acetate + ATP	R00229	4.1	0.3	mmol/g*h
NADPH -> NADH	R00105	-4.2	0.8	mmol/g*h
O2 + 2 NADH -> 2 PO + ATP		11.7	1	mmol/g*h
Biomass		0.67	0.01	mmol/g*h

Graph

Loading...

Reaction	KEGG ID	Flux	Std	Unit
GLC + ATP -> G6P	R02738	9.7	0.3	mmol/g*h
G6P -> 6PG + NADPH	R01528	2.9	0.1	mmol/g*h
6PG -> P5P + CO2 + NADPH	R10221	2.4	0.2	mmol/g*h
G6P -> F6P	R02740	6.7	0.2	mmol/g*h
6PG -> T3P + PYR	R05605	0.5	0.2	mmol/g*h
F6P + ATP -> 2 T3P		7.7	0.3	mmol/g*h
2 P5P -> S7P + T3P		0.6	0.1	mmol/g*h
P5P + E4P -> F6P + T3P	R01067	0.4	0.1	mmol/g*h
S7P + T3P -> E4P + F6P	R01827	0.6	0.1	mmol/g*h
T3P -> PGA + ATP + NADH		16.2	0.5	mmol/g*h
PGA -> PEP		14.8	0.5	mmol/g*h
PEP -> PYR + ATP	R00200	11.6	0.5	mmol/g*h
PYR -> AcCoA + CO2 + NADH	R01196	10.3	0.6	mmol/g*h
OAA + AcCoA -> ICT		3.8	0.6	mmol/g*h
ICT -> OGA + CO2 + NADPH	R00267	3.8	0.6	mmol/g*h
OGA -> FUM + CO2 + 1.5 ATP + 2 NADH		2.9	0.6	mmol/g*h
FUM -> MAL	R01082	2.9	0.6	mmol/g*h
MAL -> OAA + NADH	R00342	2.5	0.4	mmol/g*h
MAL -> PYR + CO2 + NADH	R00214	0.4	0.4	mmol/g*h
OAA + ATP -> PEP + CO2	R00341	0.4	0.2	mmol/g*h
PEP + CO2 -> OAA	R00341	3	0.4	mmol/g*h
AcCoA -> Acetate + ATP	R00229	4.8	0.5	mmol/g*h
NADPH -> NADH	R00105	-2.5	1.1	mmol/g*h
O2 + 2 NADH -> 2 PO + ATP		17.5	1.8	mmol/g*h
Biomass		0.74	0.03	mmol/g*h

Graph

Loading...

Reaction	KEGG ID	Flux	Std	Unit
GLC + ATP -> G6P	R02738	9.6	0.1	mmol/g*h
G6P -> 6PG + NADPH	R01528	2.9	0.1	mmol/g*h
6PG -> P5P + CO2 + NADPH	R10221	2.4	0.1	mmol/g*h
G6P -> F6P	R02740	6.6	0.1	mmol/g*h
6PG -> T3P + PYR	R05605	0.5	0.2	mmol/g*h
F6P + ATP -> 2 T3P		7.6	0.2	mmol/g*h
2 P5P -> S7P + T3P		0.7	0	mmol/g*h
P5P + E4P -> F6P + T3P	R01067	0.4	0	mmol/g*h
S7P + T3P -> E4P + F6P	R01827	0.7	0	mmol/g*h
T3P -> PGA + ATP + NADH		16	0.2	mmol/g*h
PGA -> PEP		14.7	0.3	mmol/g*h
PEP -> PYR + ATP	R00200	11.2	0.3	mmol/g*h
PYR -> AcCoA + CO2 + NADH	R01196	10.4	0.3	mmol/g*h
OAA + AcCoA -> ICT		3.6	0.3	mmol/g*h
ICT -> OGA + CO2 + NADPH	R00267	3.6	0.3	mmol/g*h
OGA -> FUM + CO2 + 1.5 ATP + 2 NADH		2.7	0.3	mmol/g*h
FUM -> MAL	R01082	2.7	0.3	mmol/g*h
MAL -> OAA + NADH	R00342	1.9	0.2	mmol/g*h
MAL -> PYR + CO2 + NADH	R00214	0.9	0.3	mmol/g*h
OAA + ATP -> PEP + CO2	R00341	0	0.2	mmol/g*h
PEP + CO2 -> OAA	R00341	3	0.3	mmol/g*h
AcCoA -> Acetate + ATP	R00229	5.1	0.1	mmol/g*h
NADPH -> NADH	R00105	-2.4	0.8	mmol/g*h
O2 + 2 NADH -> 2 PO + ATP		17.3	0.9	mmol/g*h
Biomass		0.73	0.01	mmol/g*h

Graph

Loading...

Manuscript title	Impact of global transcriptional regulation by arcA, arcB, cra, crp, cya, fnr, and mlc on glucose catabolism in Escherichia coli.
PubMed ID	15838044
Journal	Journal of Bacteriology
Year	2005
Authors	Annik Perrenoud and Uwe Sauer
Affiliations	Institute of Biotechnology, ETH, Zurich, Switzerland
Keywords	Escherichia coli, aerobic glucose catabolism
Full text article	Perrenoud_2005.pdf
Project name	not specified

Organism	Escherichia coli
Strain	BW25113 and mutants
Data type	flux measurements
Data units	(mmol/g.h)
Execution date	not specified

APA	KiMoSys (https://kimosys.org). (2020, April 26). Data EntryID 64 (Escherichia coli). https://doi.org/10.34619/z4tb-zk02
MLA	KiMoSys (https://kimosys.org). "Data EntryID 64 (Escherichia coli)." 2024, https://doi.org/10.34619/z4tb-zk02
ISO-690	KiMoSys (https://kimosys.org). Data EntryID 64 (Escherichia coli). [online], [Accessed 26 April 2024]. Available from: https://doi.org/10.34619/z4tb-zk02
BibTeX	@misc{ KiMoSysDataEntryID64, author = "{KiMoSys (https://kimosys.org)}", title = "Data EntryID 64 (Escherichia coli)", url = https://doi.org/10.34619/z4tb-zk02", doi = "10.34619/z4tb-zk02", note = "[Online; accessed 26-April-2024]" }

Detail View - Data AccessID 64

General Information

Experiment Description

Experimental Details

Submission and curation

Associated Models

Associate models to data