Synthetic mineral medium: 4.0 g/L Na2SO4, 5.36 g/L (NH4)2SO4, 1.0 g/L NHCl, 7.3 g/L K2HPO4, 1.8 g/L NaH2PO4 H2O, 120 g/L (NH4)2-H-citrate, 4.0 mL/L MgSO4 (1M), 6.0 ml/L trace element solution, 0.02 g/L thiamine, 20.0 g/L glucose.

The concentrations of the extracellular metabolites (glucose, acetate) and most of the intracellular metabolites were determined as describe previously [1]. Improved analysis of the adenine nucleotides was summarized by Mailinger et al. [2] and Meyer et al. [3]. The metaboli ... tes and cometabolites from the pentose-phosphate pathway (6PG, NADP+, and NADPH) were measured according to Vaseghi et al. [4].
See also Figure 1 of the original model.

-------------References------------
[1] Theobals U., Mailinger W., Baltes M., Rizzi M., Reuss M., 1997. In vivo analysis of metabolic dynamics in Saccharomyces cerevisae: I. Experimental observations. Biotechnol Bioeng. 55: 305-316. http://doi.org/crtbrx
[2] Mailinger W., Baumeister A., Reuss M., Rizzi M., 1998. Rapid and highly automated determination of adenine and pyridine nucleotides in extracts of Saccharomyces cerevisae using a micro robotic sample preparation-HPLC system. J. Biotechnol. 63: 155-166. http://doi.org/bvbfcn
[3] Meyer S., Noisommit-Rizzi N., Reuss M., Neubauer P., 1999. Optimized analysis of intracellular adenosine and guanosine phosphate in Escherichia coli. Anal Biochem. 271: 43-52. http://doi.org/c7nb99
[4] Vaseghi S., Baumeister A., Rizzi M., Reuss M., 1999. In vivo dynamics of the pentose phosphate pathway in Saccharomyces cerevisae. Metab Eng. 1: 128-140. http://doi.org/bvvrdg