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General Information
Manuscript title Analysis of Escherichia coli Anaplerotic Metabolism and Its Regulation Mechanisms From the Metabolic Responses to Altered Dilution Rates and Phosphoenolpyruvate Carboxykinase Knockout.
PubMed ID 12966569
Journal Biotechnology and Bioengineering
Year 2003
Authors Yang C, Hua Q, Baba T, Mori H, Shimizu K
Affiliations Metabolome Unit, Institute for Advanced Biosciences, Keio University, Tsuruoka 997-0017, Japan.
Keywords Escherichia coli; metabolic flux; 13C labeling; anaplerotic reaction; phosphoenolpyruvate carboxykinase; in vivo regulation
Full text article Yang2003.pdf
Project name not specified


Experiment Description
Organism Escherichia coli
Strain W3110 and pck mutant
Data type flux measurements
Data units (mmol/g-dry cell weight/h)
Execution date not specified


Experimental Details
Temperature (°C) 37
pH 7.0
Carbon source glucose,
Culture mode chemostat
Process condition aerobic
Dilution rate (h⁻¹) 0.1, 0.32, 0.55 (WT ) and 0.1 (pck)
Working volume (L) 1.0
Biomass concentration (g/L) see worksheet.
Medium composition

Medium containing (per liter): 5.0 g of glucose, 1.0 g of NH4Cl, 2.7 g of (NH4)2SO4,6.8gofNa2HPO4,3.0gofKH2PO4, 0.6 g of NaCl, 0.2 g ofMgSO4 7H2O, 1.0 µg of thiamine HCl, 2.0 µL of polypropylene glycol 2000 as an antiform agent, and 10 mL of trace element solution [1].

General protocol information Flux analysis method: 13C constrained MFA

Platform: NMR

Methods description - Notes

MFA analysis: The carbon flux distribution in the bioreaction network was determined as a best fit to all extracellular flux measurements, the macromolecular biomass composition, and the relative intensities of the 13C-13C scalar coupling multiplets of the aforementioned 47 ...

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Submission and curation

Entered by: Administrator KiMoSysFirst name: Administrator
Affiliation: INESC-ID/IST
Homepage: http://kdbio.inesc-id.pt/kimosys
Interests: mathematical modeling, accessible data, use of data

Created: 2018-07-21 16:52:41 UTC

Updated: 2020-04-24 16:10:36 UTC

Version: 1

Status: (reviewed) 2018-07-21 16:56:08 UTC

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Associated Models

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Model
EntryID
Model name Category Model Type Data used for Access Json
16 glycolysis Escherichia coli model Metabolism ordinary differential equations Model validation {"id":16,"organism_id":38,"comments":"","sbml_file_name":"Kadir_2010.pdf","article_file_name":null,"category":"Metabolism","used_for":"---\n- Model validation\n","model_type":"ordinary differential equations","pubmed_id":"21092096","dilution_rate":"—","name_of_model":"glycolysis Escherichia coli model","manuscript_title":"Modeling and simulation of the main metabolism in Escherichia coli and its several single-gene knockout mutants with experimental verification.","authors":"Tuty AA Kadir, Ahmad A Mannan, Andrzej M Kierzek, Johnjoe McFadden and Kazuyuki Shimizu","journal":"Microbial Cell Factories","affiliation":"Department of Bioscience and Bioinformatics, Kyushu Institute of Technology, Iizuka, Fukuoka 820-8502, Japan","project_name":"","biomodels_id":"","keywords":"Escherichia coli, single-gene knockouts, main central metabolism and TCA","software":"http://www.matlab.com (MATLAB)","control":"2018-07-21T20:34:09.507Z","main_organism":"Escherichia coli","year":2010,"combine_archive_file_name":null,"combine_archive_content_type":null,"combine_archive_file_size":null,"combine_archive_updated_at":null,"review_journal_id":null,"doi":null} Administrator KiMoSys



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