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Manuscript title – title of the original publication where the data are published or a general title if not published yet. PubMed ID - the PubMed ID related to this manuscript. Journal - the journal name of the manuscript. Year - the year of publication. Authors - the authors of the manuscript. Affiliations - the affilation(s) of the first author. Keywords - general keywords that characterize the data. Full text article - article file where the data are described. Project name - the comprehensive name (acronym) of the project (if the data is part of a general project).
Absolute Metabolite Concentrations and Implied Enzyme Active Site Occupancy in Escherichia coli.
This section provides basic information about the data.
Organism - scientific name of the organism used to generate the data. Strain - name of the strain used to generate the data. Data type - experimental data type uploaded in this EntryID. Data units - units of the data set. Execution date - experimental start date of the data.
Temperature - the temperature of the experimental conditions in this data. pH - the pH of the experimental conditions in this data. Carbon source - the carbon source name used in this study. Culture mode - how the culture growth in the experiment. Process condition - process condition of the experiment. Dilution rate - dilution rate of the experiment for chemostat culture. Working volume - working volume used. Biomass concentration - biomass concentration used in this experiment. Medium composition - detailed concentration components used in the culture medium. General protocol information - overview about the experimental techniques and instrument type. Methods description and Notes - a summary description of the overall experiment, protocolos steps that have been performed and any other pertinent information helpful for researches. Description taken and adapted from the original manuscript. Data file - freely available structured Excel data file (contains metadata + dataset). Alternative format(s) - freely available data for download in delimited format files (.txt and/or .csv) as part of the normal Excel data file (contains dataset). The .txt files can be used as input for the "Tools" tab
and the .csv files are in the SBtab (http://www.sbtab.net.) format. The time-series data files (.csv) are specified in the machine-readable format for Copasi tool. Related Data (if available)- data entities with the same PubMed ID, organism or project name.
glucose, glycerol, acetate
Dilution rate (h-1)
Working volume (L)
Biomass concentration (g/L)
Cultures were grown to OD650 of 0.35.
Gutnick minimal complete medium with 4g/L of glucose, glycerol, or acetate as the carbon source. Complete media formulation was 4.7g/L KH2PO4, 13.5g/L K2HPO4, 1 g/L K2SO4, 0.1 g/L MgSO4·7H2O, and final concentrations of 10 mM NH4Cl, and 4g/L of the carbon source (acetate was added as 6.65 g/L of potassium acetate)
General protocol information
This liquid culture was grown to OD650~0.1, at which time it was transferred to filter culture as follows:for each filter culture, 5 mL of liquid culture was passed through an 82 mm diameter round nylon filter and the placed cell-side up onto a plate.
Cells were filter (0.45-µm), washed with 10 mL of Milli-Q water and plunged into 1 mL of methanol containing 10µL of 0.56 mM PIPES.