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General Information info

Manuscript title Absolute Metabolite Concentrations and Implied Enzyme Active Site Occupancy in Escherichia coli.
PubMed ID 19561621
Journal Nature Chemical Biology
Year 2009
Authors Bryson D Bennett, Elizabeth H Kimball, Melissa Gao, Robin Osterhout, Stephen J Van Dien, and Joshua D Rabinowitz
Affiliations Department of Chemistry and Lewis-Sigler Institute for Integrative Genomics, Princeton
Keywords not specified
Full text article no file uploaded
Project name not specified

Experiment Description info

Organism Escherichia coli
Strain WT K-12 strain NCM3722 of E. coli
Data type metabolites at steady-state
Data units mM
Execution date not specified

Experimental Details info

Temperature (0C) 37
pH not specified
Carbon source glucose, glycerol, acetate
Culture mode batch
Process condition aerobic
Dilution rate (h-1) -
Working volume (L) 0.05
Biomass concentration (g/L) Cultures were grown to OD650 of 0.35.
Medium composition

Gutnick minimal complete medium with 4g/L of glucose, glycerol, or acetate as the carbon source. Complete media formulation was 4.7g/L KH2PO4, 13.5g/L K2HPO4, 1 g/L K2SO4, 0.1 g/L MgSO4·7H2O, and final concentrations of 10 mM NH4Cl, and 4g/L of the carbon source (acetate was added as 6.65 g/L of potassium acetate)

General protocol information Sampling method: This liquid culture was grown to OD650~0.1, at which time it was transferred to filter culture as follows:for each filter culture, 5 mL of liquid culture was passed through an 82 mm diameter round nylon filter and the placed cell-side up onto a plate.

Quenching procedure: Cells were filter (0.45-µm), washed with 10 mL of Milli-Q water and plunged into 1 mL of methanol containing 10µL of 0.56 mM PIPES.

Extraction technique: acidic acetonitrile-methanol

Sample analyzing method: NMR, LC-ESI-MS

Methods description - Notes

See more details in Supplementary Methods online.

Data file
Downloadmetabolites KIMODATAID92_v2.xlsx
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Organism


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Submission and curation info

Entered by Administrator KiMoSysFirst name: Administrator
Affiliation: INESC-ID/IST
Homepage: http://kdbio.inesc-id.pt/kimosys
Interests: mathematical modeling, accessible data, use of data

Created 2018-07-06 10:59:59 UTC

Updated 2018-07-06 11:10:59 UTC

Version 2

Status (reviewed) 2018-07-06 11:01:53 UTC




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