Detail View - Data AccessID 92
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General Information
| Manuscript title | Absolute Metabolite Concentrations and Implied Enzyme Active Site Occupancy in Escherichia coli. |
| PubMed ID | 19561621 |
| Journal | Nature Chemical Biology |
| Year | 2009 |
| Authors | Bryson D Bennett, Elizabeth H Kimball, Melissa Gao, Robin Osterhout, Stephen J Van Dien, and Joshua D Rabinowitz |
| Affiliations | Department of Chemistry and Lewis-Sigler Institute for Integrative Genomics, Princeton |
| Keywords | not specified |
| Full text article | no file uploaded |
| Project name | not specified |
Experiment Description
| Organism | Escherichia coli |
| Strain | WT K-12 strain NCM3722 of E. coli |
| Data type | metabolites at steady-state |
| Data units | mM |
| Execution date | not specified |
Experimental Details
| Temperature (°C) | 37 |
| pH | not specified |
| Carbon source | glucose, glycerol, acetate |
| Culture mode | batch |
| Process condition | aerobic |
| Dilution rate (h⁻¹) | - |
| Working volume (L) | 0.05 |
| Biomass concentration (g/L) | Cultures were grown to OD650 of 0.35. |
| Medium composition | Gutnick minimal complete medium with 4g/L of glucose, glycerol, or acetate as the carbon source. Complete media formulation was 4.7g/L KH2PO4, 13.5g/L K2HPO4, 1 g/L K2SO4, 0.1 g/L MgSO4·7H2O, and final concentrations of 10 mM NH4Cl, and 4g/L of the carbon source (acetate was added as 6.65 g/L of potassium acetate) |
| General protocol information |
Sampling method:
This liquid culture was grown to OD650~0.1, at which time it was transferred to filter culture as follows:for each filter culture, 5 mL of liquid culture was passed through an 82 mm diameter round nylon filter and the placed cell-side up onto a plate. Quenching procedure: Cells were filter (0.45-µm), washed with 10 mL of Milli-Q water and plunged into 1 mL of methanol containing 10µL of 0.56 mM PIPES. Extraction technique: acidic acetonitrile-methanol Sample analyzing method: NMR, LC-ESI-MS |
| Methods description - Notes | See more details in Supplementary Methods online. |
| Data file |
 KIMODATAID92_v2.xlsx
|
| Alternative format(s) |
no file uploaded |
| Export metadata |
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Submission and curation
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Administrator KiMoSysFirst name: Administrator
Affiliation: INESC-ID/IST
Homepage: http://kdbio.inesc-id.pt/kimosys
Interests: mathematical modeling, accessible data, use of data
Created: 2018-07-06 10:59:59 UTC
Updated: 2020-04-24 16:10:35 UTC
Version: 2
Status: (reviewed) 2018-07-06 11:01:53 UTC
Views: 294
Downloads: 73
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