Repository » Data AccessID 92
Detail View - Data AccessID 92

Back

General Information
Manuscript title Absolute Metabolite Concentrations and Implied Enzyme Active Site Occupancy in Escherichia coli.
PubMed ID 19561621
Journal Nature Chemical Biology
Year 2009
Authors Bryson D Bennett, Elizabeth H Kimball, Melissa Gao, Robin Osterhout, Stephen J Van Dien, and Joshua D Rabinowitz
Affiliations Department of Chemistry and Lewis-Sigler Institute for Integrative Genomics, Princeton
Keywords not specified
Full text article no file uploaded
Project name not specified


Experiment Description
Organism Escherichia coli
Strain WT K-12 strain NCM3722 of E. coli
Data type metabolites at steady-state
Data units mM
Execution date not specified


Experimental Details
Temperature (°C) 37
pH not specified
Carbon source glucose, glycerol, acetate
Culture mode batch
Process condition aerobic
Dilution rate (h⁻¹) -
Working volume (L) 0.05
Biomass concentration (g/L) Cultures were grown to OD650 of 0.35.
Medium composition

Gutnick minimal complete medium with 4g/L of glucose, glycerol, or acetate as the carbon source. Complete media formulation was 4.7g/L KH2PO4, 13.5g/L K2HPO4, 1 g/L K2SO4, 0.1 g/L MgSO4·7H2O, and final concentrations of 10 mM NH4Cl, and 4g/L of the carbon source (acetate was added as 6.65 g/L of potassium acetate)
General protocol information Sampling method: This liquid culture was grown to OD650~0.1, at which time it was transferred to filter culture as follows:for each filter culture, 5 mL of liquid culture was passed through an 82 mm diameter round nylon filter and the placed cell-side up onto a plate.

Quenching procedure: Cells were filter (0.45-µm), washed with 10 mL of Milli-Q water and plunged into 1 mL of methanol containing 10µL of 0.56 mM PIPES.

Extraction technique: acidic acetonitrile-methanol

Sample analyzing method: NMR, LC-ESI-MS
Methods description - Notes

See more details in Supplementary Methods online.
Data file
KIMODATAID92_v2.xlsx Preview file
Alternative format(s)
 no file uploaded
Export metadata
 RDF: metadataDataEntryID92.rdf
XML: metadataDataEntryID92.xml
Plain text: metadataDataEntryID92.txt
Share | Cite Data EntryID 92


Related Data: AccessID 30 | AccessID 35 | AccessID 38 | AccessID 41 | AccessID 44 | AccessID 51 | AccessID 52 | AccessID 54 | AccessID 63 | AccessID 64 | AccessID 65 | AccessID 67 | AccessID 68 | AccessID 74 | AccessID 75 | AccessID 78 | AccessID 79 | AccessID 80 | AccessID 86 | AccessID 87 | AccessID 96 | AccessID 101 | AccessID 102 | AccessID 103 | AccessID 104 | AccessID 105 | AccessID 106 | AccessID 107 | AccessID 108 | AccessID 109 | AccessID 110 | AccessID 112 | AccessID 116 | AccessID 118 | AccessID 119 | AccessID 125 | AccessID 126


Submission and curation

Entered by: Administrator KiMoSysFirst name: Administrator
Affiliation: INESC-ID/IST
Homepage: http://kdbio.inesc-id.pt/kimosys
Interests: mathematical modeling, accessible data, use of data

Created: 2018-07-06 10:59:59 UTC

Updated: 2020-04-24 16:10:35 UTC

Version: 2

Status: (reviewed) 2018-07-06 11:01:53 UTC

Views: 293

Downloads: 73



Associated Models

Here we can find relevant models associated with Data EntryID 92:

Model
EntryID		 Model name Category Model Type Data used for Access Json



Associate models to data

- Several models can be associated.

New Model



Back | Top