Detail View - Data AccessID 107
General Information
Manuscript title | Metabolic flux analysis of pykF gene knockout Escherichia coli based on 13C-labeling experiments together with measurements of enzyme activities and intracellular metabolite concentrations. |
PubMed ID | 12802531 |
Journal | Appl Micriobiol Biotechnology |
Year | 2004 |
Authors | Al Zaid Siddiquee K, Arauzo-Bravo MJ, Shimizu K. |
Affiliations | Department of Biochemical Engineering and Science, Kyushu Institute of Technology,Iizuka, 820–8502 Fukuoka, Japan |
Keywords | Dilution Ratem Intracellular Metabolite, Metabolic Flux Analysis, Oxidative Pentose Phosphate Pathway, Proteinogenic Amino Acid |
Full text article | no file uploaded |
Project name | not specified |
Experiment Description
Organism | Escherichia coli |
Strain | BW25113 and pyk mutant |
Data type | flux measurements |
Data units | (mmol/g-dry cell weight/h) |
Execution date | not specified |
Experimental Details
Temperature (°C) | 37 |
pH | 7.0 |
Carbon source | glucose |
Culture mode | chemostat |
Process condition | aerobic |
Dilution rate (h⁻¹) | 0.1 |
Working volume (L) | 0.5 |
Biomass concentration (g/L) | see worksheet. |
Medium composition | Minimal medium consisting of 5 g glucose per liter, 48 mM Na2HPO4,22mMKH2PO4, 10 mM NaCl, and40 mM (NH4)SO4, was used. For minimal medium, the following components were filter-sterilized separately and then added (per liter of final volume): 1 ml 1 M MgSO4, 1 ml 0.1 mM CaCl2, 1ml vitamin B1 (1 mg/l stock) and 10 ml trace element solution containing (per liter): 0.55 g CaCl2, 1 g FeCl3, 0.1 g MnCl2·4H2O, 0.17 g ZnCl2, 0.043 g CuCl2·2H2O, 0.06 g CoCl2·2H2O, and 0.06 g Na2MoO4·2H2O. |
General protocol information |
Flux analysis method:
13C constrained MFA Platform: NMR, GC-MS |
Methods description - Notes | The isotopomer balance systems were described using isotopomer mapping matrices [1] and solved through an iterative scheme, based on the approach of [1]. The isotopomers of the proteinogenic amino acids were used to generate NMR synthetic patterns. For the representation of ... Read more |
Data file |
KIMODATAID107_v1.xlsx
|
Alternative format(s) |
no file uploaded |
Export metadata |
RDF:
metadataDataEntryID107.rdf
XML: metadataDataEntryID107.xml Plain text: metadataDataEntryID107.txt |
Related Data: AccessID 30 | AccessID 35 | AccessID 38 | AccessID 41 | AccessID 44 | AccessID 51 | AccessID 52 | AccessID 54 | AccessID 63 | AccessID 64 | AccessID 65 | AccessID 67 | AccessID 68 | AccessID 74 | AccessID 75 | AccessID 78 | AccessID 79 | AccessID 80 | AccessID 86 | AccessID 87 | AccessID 92 | AccessID 96 | AccessID 101 | AccessID 102 | AccessID 103 | AccessID 104 | AccessID 105 | AccessID 106 | AccessID 108 | AccessID 109 | AccessID 110 | AccessID 112 | AccessID 116 | AccessID 118 | AccessID 119 | AccessID 125 | AccessID 126
Entered by:
Administrator KiMoSysFirst name: Administrator
Affiliation: INESC-ID/IST
Homepage: http://kdbio.inesc-id.pt/kimosys
Interests: mathematical modeling, accessible data, use of data
Created: 2018-07-21 20:09:18 UTC
Updated: 2020-04-24 16:10:36 UTC
Version: 1
Status: (reviewed) 2018-07-21 20:19:41 UTC
Views: 338
Downloads: 72
Associated Models
Here we can find relevant models associated with Data EntryID 107:
Model EntryID |
Model name | Category | Model Type | Data used for | Access |
---|---|---|---|---|---|
16 | glycolysis Escherichia coli model | Metabolism | ordinary differential equations | Model validation |
Associate models to data
- Several models can be associated.