General Information info
|Manuscript title||Effect of a single-gene knockout on the metabolic regulation in Escherichia coli for D-lactate production under microaerobic condition.|
|Authors||Jiangfeng Zhu, Kazuyuki Shimizu|
|Affiliations||Department of Biochemical Engineering & Science, Kyushu Institute of Technology, Iizuka, Fukuoka 820-8502, Japan|
|Keywords||Escherichia coli, Enzyme activity, Metabolite concentration, Metabolic flux analysis, Lactate production, Pyruvate metabolism|
|Full text article||Zhu_2005.pdf|
|Project name||not specified|
Experiment Description info
|Strain||BW25113, pflA, pta, ppc, adhE and pykF mutants|
|Data type||metabolites at steady-state|
|Data units||yield on glucose (g/g)%|
|Execution date||not specified|
Experimental Details info
|Dilution rate (h-1)||—|
|Working volume (L)||1.0|
|Biomass concentration (g/L)||See worksheet for the different strains. Cell dry weight was determined by measuring the optical density at 600 nm.|
Reactor medium was a minimal medium containing (per liter) 2 g of Na2SO4, 2.5 g of (NH4)2SO4, 0.5 g of NH4Cl, 7.3 g of K2HPO4, 3.6 g of NaH2PO4. The following components were sterilized by passing through a 0.2 μm pore-size filter (per liter of final medium): 3 ml of 1 M MgSO4, and 3 ml trace element solution containing (per liter) 0.5 g of CaCl2·H2O, 0.18 g of ZnSO4·7H2O, 0.1 g of MnSO4·H2O, 21.1 g of Na2EDTA, 16.7 g of FeCl3·6H2O, 0.16 g of CuSO4·5H2O and 0.18 g of CoCl2·6H2O. Glucose was used as a carbon source and the initial concentration was about 10 g/L.
|General protocol information||
Samples were centrifuged for 10 min at 4 °C and 6000×g to remove the cells for extracellular metabolite analysis.
Quenching procedure: —
Extraction technique: not used
Sample analyzing method: enzymatic
|Methods description - Notes||
The concentrations of D-lactate, acetate, formate and succinate were measured using capillary electrophoresis (CE) (Agilent, Germany).
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Here we can find relevant models associated with Data EntryID 101:
|Model name||Category||Model Type||Data used for||Access|
Authors: Yu Matsuoka and Hiroyuki Kurata
Original paper: Modeling and simulation of the redox regulation of the metabolism in Escherichia coli at different oxygen concentrations.
|E. coli redox regulation model||Metabolism||ordinary differential equations||Model building and Model validation|