Repository » Data AccessID 102
Detail View - Data AccessID 102

Back

General Information
Manuscript title Effect of a single-gene knockout on the metabolic regulation in Escherichia coli for D-lactate production under microaerobic condition.
PubMed ID 15781419
Journal Metabolic Engineering
Year 2005
Authors Jiangfeng Zhu, Kazuyuki Shimizu
Affiliations Department of Biochemical Engineering & Science, Kyushu Institute of Technology, Iizuka, Fukuoka 820-8502, Japan.
Keywords Escherichia coli, Enzyme activity, Metabolite concentration, Metabolic flux analysis, Lactate production, Pyruvate metabolism
Full text article Zhu_2005.pdf
Project name not specified


Experiment Description
Organism Escherichia coli
Strain BW25113, pflA, pta, ppc, adhE and pykF mutants
Data type enzyme/protein concentrations
Data units mmol/min mg protein
Execution date not specified


Experimental Details
Temperature (°C) 37
pH 7.0
Carbon source glucose,
Culture mode batch
Process condition aerobic
Dilution rate (h⁻¹)
Working volume (L) 1.0
Biomass concentration (g/L) Cell dry weight was determined by measuring the optical density at 600 nm. See worksheet.
Medium composition

not specified

General protocol information Measurement method: colorimetric assays

Methods description - Notes

The culture broth with a volume corresponding to about 100 mg (dry weight) of cells was centrifuged (4 °C, 10 min at 10,000×g) and washed twice with 20 mM Tris-HCl buffer (pH at 7.0). Cells were resuspended in Tris (100 mM) -HCl buffer (pH at 7.0) containing KCl (20 mM), MnS ...

Read more
Data file
KIMODATAID102_v4.xlsx Preview file
Alternative format(s)
no file uploaded
Export metadata
RDF: metadataDataEntryID102.rdf
XML: metadataDataEntryID102.xml
Plain text: metadataDataEntryID102.txt
Share | Cite Data EntryID 102


Related Data: AccessID 30 | AccessID 35 | AccessID 38 | AccessID 41 | AccessID 44 | AccessID 51 | AccessID 52 | AccessID 54 | AccessID 63 | AccessID 64 | AccessID 65 | AccessID 67 | AccessID 68 | AccessID 74 | AccessID 75 | AccessID 78 | AccessID 79 | AccessID 80 | AccessID 86 | AccessID 87 | AccessID 92 | AccessID 96 | AccessID 101 | AccessID 103 | AccessID 104 | AccessID 105 | AccessID 106 | AccessID 107 | AccessID 108 | AccessID 109 | AccessID 110 | AccessID 112 | AccessID 116 | AccessID 118 | AccessID 119 | AccessID 125 | AccessID 126


Submission and curation

Entered by: Administrator KiMoSysFirst name: Administrator
Affiliation: INESC-ID/IST
Homepage: http://kdbio.inesc-id.pt/kimosys
Interests: mathematical modeling, accessible data, use of data

Created: 2018-07-20 13:04:40 UTC

Updated: 2020-09-01 20:52:16 UTC

Version: 4

Status: (reviewed) 2018-07-20 13:15:12 UTC

Views: 42

Downloads: 11




Associated Models

Here we can find relevant models associated with Data EntryID 102:

Model
EntryID
Model name Category Model Type Data used for Access Json



Associate models to data

- Several models can be associated.

New Model



Back | Top