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General Information info

Manuscript title Metabolic flux analysis in a nonstationary system: fed-batch fermentation of a high yielding strain of E. coli producing 1,3-propanediol.
PubMed ID 17400499
Journal Metabolic Engineering
Year 2007
Authors Maciek R. Antoniewicz, David F. Kraynie, Lisa A. Laffend, Joanna González-Lergier, Joanne K. Kelleher and Gregory Stephanopoulos
Affiliations Department of Chemical Engineering, Bioinformatics and Metabolic Engineering Laboratory, Massachusetts Institute of Technology, Cambridge
Keywords E. coli, extracelullar metabolites, HPLC
Full text article Downloadarticle Antoniewicz_2007.pdf
Project name not specified

Experiment Description info

Organism Escherichia coli
Strain K-12 overproduce 1,3-propanediol
Data type time-series data of metabolites
Data units mM
Execution date not specified

Experimental Details info

Temperature (0C) 34.0
pH 6.8 ± 0.04
Carbon source glucose,
Culture mode fed-batch
Process condition aerobic
Dilution rate (h-1)
Working volume (L) 1.0
Biomass concentration (g/L) 3.0
Medium composition

Defined medium contained (per liter): 2.8g KH2PO4, 2.0 g citric acid monohydrate, 1.1 g MgSO4·7H2O, 0.2 g FeSO4·7H2O, 0.2 g CaCl2·2H2O, 0.5 g Yeast Extract (BBL), 1 mL Balch’s Modified Trace Metals (100x), 0.5 mL sulfuric acid (98%), 1.5 mL phosphoric acid (85%), 0.4 mL Mazu DF204 antifoam, appropriate vitamin B12, and 2 mL spectinomycin (50 mg/mL).

General protocol information Sampling method: Periodically taken during the fed-batch

Quenching procedure:

Extraction technique: not used

Sample analyzing method: HPLC-UV/RI

Methods description - Notes

Concentrations of glucose, glycerol, 1,3-propanediol (PDO), acetate, citrate, and pyruvate in the medium samples were measured by high-performance liquid chromatography (Waters HPLC, Shodex SH1011 sugar column, RI detector).

Data file
Downloadmetabolites KIMODATAID78_v0.xlsx
Alternative format(s)
KIMODATAID78_metabolites_timeseries.csv
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Organism


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Submission and curation info

Entered by Administrator KiMoSysFirst name: Administrator
Affiliation: INESC-ID/IST
Homepage: http://kdbio.inesc-id.pt/kimosys
Interests: mathematical modeling, accessible data, use of data

Created 2013-07-05 14:28:29 UTC

Updated 2014-05-02 23:36:47 UTC

Version 0

Status (reviewed) 2013-12-06 17:17:38 UTC




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