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General Information
| Manuscript title | Metabolic flux analysis for a ppc mutant Escherichia coli based on 13C-labelling experiments together with enzyme activity assays and intracellular metabolite measurements. |
| PubMed ID | 15158257 |
| Journal | FEMS Microbiology Letters |
| Year | 2004 |
| Authors | Lifeng Peng, Marcos J. Arauzo-Bravo, Kazuyuki Shimizu |
| Affiliations | Department of Biochemical Engineering and Science, Kyushu Institute of Technology, Iizuka, Fukuoka 820-8502, Japan. |
| Keywords | ppc mutant Escherichia coli, 2-Dimensional nuclear magnetic resonance, Gas chromatography-mass spectrometry, Metabolic flux analysis |
| Full text article | no file uploaded |
| Project name | not specified |
Experiment Description
| Organism | Escherichia coli |
| Strain | BW25113 and ppc mutant |
| Data type | metabolites at steady-state |
| Data units | mM |
| Execution date | not specified |
Experimental Details
| Temperature (°C) | 37 |
| pH | 7.0 |
| Carbon source | glucose |
| Culture mode | chemostat |
| Process condition | aerobic |
| Dilution rate (h⁻¹) | 0.2 |
| Working volume (L) | 1.0 |
| Biomass concentration (g/L) | Yield of biomass on glucose (g/g): 0,48 (WT) and 0,55 (ppc) |
| Medium composition | M9 minimal medium as described previously [3]. |
| General protocol information |
Sampling method:
Cells were harvested by centrifugation at 10,000 g for 10 min. Quenching procedure: Washed twice with 100 mM Tris-HCl (pH 7.0) containing 20 mM KCl, 5 mM MnSO4, 2 mM DTT and 0.1 mM EDTA, and then resuspended in the same buffer (ca. 15 g wet cells in 50 ml buffer solution). Extraction technique: sonication Sample analyzing method: enzymatic |
| Methods description - Notes | Intracellular metabolite concentrations in quenched cell broth were measured by enzymatic procedures using a fluorescence spectrophotometer (F-4010, Hitachi Co.,
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| Data file |
 KIMODATAID110_v1.xlsx
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Administrator KiMoSysFirst name: Administrator
Affiliation: INESC-ID/IST
Homepage: http://kdbio.inesc-id.pt/kimosys
Interests: mathematical modeling, accessible data, use of data
Created: 2018-07-29 10:54:27 UTC
Updated: 2020-04-24 16:10:36 UTC
Version: 1
Status: (reviewed) 2018-07-29 10:55:38 UTC
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