Repository » Data AccessID 65
Detail View - Data AccessID 65

Back

General Information
Manuscript title Nonlinear dependency of intracellular fluxes on growth rate in miniaturized continuous cultures of Escherichia coli.
PubMed ID 16461663
Journal Applied and Environmental Microbiology
Year 2006
Authors Annik Nanchen, Alexander Schicker and Uwe Sauer
Affiliations Institute of Molecular Systems Biology, ETH Zurich, Zurich, Switzerland
Keywords Escherichia coli, continuous culture, intracellular fluxes
Full text article Nanchen_2006.pdf
Project name not specified


Experiment Description
Organism Escherichia coli
Strain MG1655
Data type flux measurements
Data units (mmol/gDW.h)
Execution date not specified


Experimental Details
Temperature (°C) 37.0
pH 7.0
Carbon source glucose
Culture mode chemostat
Process condition aerobic
Dilution rate (h⁻¹) several dilution rates
Working volume (L) 0.010
Biomass concentration (g/L) 0.2-0.5
Medium composition

M9 medium contained (per liter of deionized water) 0.8 g NH4Cl, 0.5 g NaCl, 7.5 g Na2HPO4 · 2H2O, and 3.0 g KH2PO4. The following components were sterilized separately and then added (per liter of final volume): 2 ml of 1 M MgSO4, 1 ml of 0.1 M CaCl2, 0.3 ml of 1 mM filter-sterilized thiamine HCl, and 10 ml of a trace element solution containing (per liter) 1 g of FeCl3 · 6H2O, 0.18 g ZnSO4 · 7H2O, 0.12 g CuCl2 · 2H2O, 0.12 g MnSO4 · H2O, and 0.18 g CoCl2 · 6H2O. Sterilized glucose was added to a final concentration of 1 g per liter.

General protocol information Flux analysis method: 13C constrained MFA

Platform: GC-MS

Methods description - Notes

Metabolic-flux ratio analysis by GC-MS - Samples for gas chromatographymass spectrometry (GC-MS) analysis were prepared as described previously [1]. Briefly, the 13C-labeled chemostat cultures were harvested after the OD600 was stable for at least two volume changes (the min ...

------------References---------------
[1] Fischer, E., and U. Sauer. 2003. Metabolic flux profiling of Escherichia coli mutants in central carbon metabolism using GC-MS. Eur. J. Biochem. 270: 880–891. http://doi.org/dg3q64
[2] Fischer, E., N. Zamboni, and U. Sauer. 2004. High-throughput metabolic flux analysis based on gas chromatography-mass spectrometry derived 13C constraints. Anal. Biochem. 325:308–316. http://doi.org/c6kx35
[3] Zamboni, N., E. Fischer, and U. Sauer. 2005. FiatFlux—a software for metabolic flux analysis from 13C-glucose experiments. BMC Bioinformatics 6:209. http://doi.org/bj2zk2

Read more
Data file
KIMODATAID65_v0.xlsx Preview file
Alternative format(s)
KIMODATAID65_D_0.409_reaction_SBtab.csv Preview
KIMODATAID65_D_0.399_reaction_SBtab.csv Preview
KIMODATAID65_D_0.314_reaction_SBtab.csv Preview
KIMODATAID65_D_0.314_2_reaction_SBtab.csv Preview
KIMODATAID65_D_0.286_reaction_SBtab.csv Preview
KIMODATAID65_D_0.286_2_reaction_SBtab.csv Preview
KIMODATAID65_D_0.187_reaction_SBtab.csv Preview
KIMODATAID65_D_0.187_2_reaction_SBtab.csv Preview
KIMODATAID65_D_0.114_reaction_SBtab.csv Preview
KIMODATAID65_D_0.114_2_reaction_SBtab.csv Preview
KIMODATAID65_D_0.092_reaction_SBtab.csv Preview
KIMODATAID65_D_0.092_2_reaction_SBtab.csv Preview
KIMODATAID65_D_0.085_reaction_SBtab.csv Preview
KIMODATAID65_D_0.085_2_reaction_SBtab.csv Preview
KIMODATAID65_D_0.056_reaction_SBtab.csv Preview
KIMODATAID65_D_0.048_reaction_SBtab.csv Preview
KIMODATAID65_D_0.044_reaction_SBtab.csv Preview
Export metadata
RDF: metadataDataEntryID65.rdf
XML: metadataDataEntryID65.xml
Plain text: metadataDataEntryID65.txt
Share | Cite Data EntryID 65


Related Data: AccessID 30 | AccessID 35 | AccessID 38 | AccessID 41 | AccessID 44 | AccessID 51 | AccessID 52 | AccessID 54 | AccessID 63 | AccessID 64 | AccessID 67 | AccessID 68 | AccessID 74 | AccessID 75 | AccessID 78 | AccessID 79 | AccessID 80 | AccessID 86 | AccessID 87 | AccessID 92 | AccessID 96 | AccessID 101 | AccessID 102 | AccessID 103 | AccessID 104 | AccessID 105 | AccessID 106 | AccessID 107 | AccessID 108 | AccessID 109 | AccessID 110 | AccessID 112 | AccessID 116 | AccessID 118 | AccessID 119 | AccessID 125 | AccessID 126


Submission and curation

Entered by: Administrator KiMoSysFirst name: Administrator
Affiliation: INESC-ID/IST
Homepage: http://kdbio.inesc-id.pt/kimosys
Interests: mathematical modeling, accessible data, use of data

Created: 2013-06-12 15:55:08 UTC

Updated: 2020-04-24 16:10:33 UTC

Version: 0

Status: (reviewed) 2013-12-06 17:17:38 UTC

Views: 252

Downloads: 52




Associated Models

Here we can find relevant models associated with Data EntryID 65:

Model
EntryID
Model name Category Model Type Data used for Access Json



Associate models to data

- Several models can be associated.

New Model



Back | Top