KiMoSys: a web-based repository of experimental data for Kinetic Models of biological Systems

Metadata
Data EntryID	65
Entered by	Administrator KiMoSys
Date Added	2013-07-12
Version	0
General Information
Manuscript title	Nonlinear dependency of intracellular fluxes on growth rate in miniaturized continuous cultures of Escherichia coli.
PubMed ID	16461663
Journal	Applied and Environmental Microbiology
Year	2006
Authors	Annik Nanchen, Alexander Schicker and Uwe Sauer
Affiliations	Institute of Molecular Systems Biology, ETH Zurich, Zurich, Switzerland
Keywords	Escherichia coli, continuous culture, intracellular fluxes
Project name	not specified
Experiment Description
Organism	Escherichia coli
Strain	MG1655
Data type	flux measurements
Data units	(mmol/gDW.h)
Execution date	not specified
Experimental Details
Temperature (0C)	37
pH	7.0
Carbon source	glucose
Culture mode	chemostat
Process condition	aerobic
Dilution rate (h-1)	several dilution rates
Working volume (L)	0.010
Biomass concentration (g/L)	0.2-0.5
Medium composition	M9 medium contained (per liter of deionized water) 0.8 g NH4Cl, 0.5 g NaCl, 7.5 g Na2HPO4 · 2H2O, and 3.0 g KH2PO4. The following components were sterilized separately and then added (per liter of final volume): 2 ml of 1 M MgSO4, 1 ml of 0.1 M CaCl2, 0.3 ml of 1 mM filter-sterilized thiamine HCl, and 10 ml of a trace element solution containing (per liter) 1 g of FeCl3 · 6H2O, 0.18 g ZnSO4 · 7H2O, 0.12 g CuCl2 · 2H2O, 0.12 g MnSO4 · H2O, and 0.18 g CoCl2 · 6H2O. Sterilized glucose was added to a final concentration of 1 g per liter
General protocol information	Flux analysis method: 13C constrained MFA
	Platform: GC-MS
Methods description - Notes	Metabolic-flux ratio analysis by GC-MS - Samples for gas chromatographymass spectrometry (GC-MS) analysis were prepared as described previously [1]. Briefly, the 13C-labeled chemostat cultures were harvested after the OD600 was stable for at least two volume changes (the minimum continuous operation was seven volume changes). Cell pellets were hydrolyzed in 6MHCl at 105°C for 24 h in sealed microtubes. The hydrolysates were dried under a stream of air at around 60°C and then derivatized at 85°C in 30 µ l dimethylformamide (Fluka, Switzerland) and 30 µ l N-(tert-butyldimethylsilyl)-N-methyl-trifluoroacetamide with 1% (vol/vol) tert-butyldimethylchlorosilane (Fluka, Switzerland) for 60 min [2]. Derivatized amino acids were analyzed on a series 8000 GC combined with an MD 800 mass spectrometer (Fisons Instruments, Beverly, MA). The GC-MSderived mass isotope distributions of proteinogenic amino acids were then corrected for naturally occurring isotopes [1]. The corrected mass distributions were related to the in vivo metabolic activities obtained with previously described algebraic equations and statistical-data treatment of metabolic-flux ratio analysis [1] using the software Fiat Flux [3]. ------------References--------------- [1] Fischer, E., and U. Sauer. 2003. Metabolic flux profiling of Escherichia coli mutants in central carbon metabolism using GC-MS. Eur. J. Biochem. 270: 880–891. [2] Fischer, E., N. Zamboni, and U. Sauer. 2004. High-throughput metabolic flux analysis based on gas chromatography-mass spectrometry derived 13C constraints. Anal. Biochem. 325:308–316. [3] Zamboni, N., E. Fischer, and U. Sauer. 2005. FiatFlux—a software for metabolic flux analysis from 13C-glucose experiments. BMC Bioinformatics 6:209.
Data URL	http://kdbio.inesc-id.pt/kimosys/repository/65

Reaction	KEGG ID	Flux	Std	Unit
GLC + ATP -> G6P	R02738	0.91	0.12	mmol/g*h
G6P -> 6PG + NADPH	R01528	0.26	0.05	mmol/g*h
6PG -> P5P + CO2 + NADPH	R10221	0.21	0.05	mmol/g*h
G6P -> F6P	R02740	0.65	0.09	mmol/g*h
6PG -> T3P + PYR	R05605	0.05	0.05	mmol/g*h
F6P + ATP -> 2 T3P		0.77	0.11	mmol/g*h
2 P5P -> S7P + T3P		0.07	0.02	mmol/g*h
P5P + E4P -> F6P + T3P	R01067	0.05	0.02	mmol/g*h
S7P + T3P -> E4P + F6P	R01827	0.07	0.02	mmol/g*h
T3P -> PGA + ATP + NADH		1.63	0.23	mmol/g*h
PGA -> PEP		1.56	0.24	mmol/g*h
PEP -> PYR + ATP	R00200	1.45	0.23	mmol/g*h
PYR -> AcCoA + CO2 + NADH	R01196	1.47	0.27	mmol/g*h
OAA + AcCoA -> ICT		1.26	0.26	mmol/g*h
ICT -> OGA + CO2 + NADPH	R00267	1.14	0.27	mmol/g*h
OGA -> FUM + CO2 + 1.5 ATP + 2 NADH		1.09	0.27	mmol/g*h
FUM -> MAL	R01082	1.21	0.26	mmol/g*h
MAL -> OAA + NADH	R00342	1.24	0.25	mmol/g*h
MAL -> PYR + CO2 + NADH	R00214	0.1	0.04	mmol/g*h
OAA + ATP -> PEP + CO2	R00341	0.35	0.06	mmol/g*h
PEP + CO2 -> OAA	R00341	0.42	0.08	mmol/g*h
AcCoA -> Acetate + ATP	R00229	0	0	mmol/g*h
ICT + AcCoA -> MAL + FUM + NADH		0.12	0.06	mmol/g*h
NADPH -> NADH	R00105	0.84	0.78	mmol/g*h
O2 + 2 NADH -> 2 PO + ATP		3.84	0.84	mmol/g*h
Biomass		0.04	0	mmol/g*h

Graph

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Reaction	KEGG ID	Flux	Std	Unit
GLC + ATP -> G6P	R02738	1.19	0.17	mmol/g*h
G6P -> 6PG + NADPH	R01528	0.34	0.06	mmol/g*h
6PG -> P5P + CO2 + NADPH	R10221	0.27	0.07	mmol/g*h
G6P -> F6P	R02740	0.85	0.13	mmol/g*h
6PG -> T3P + PYR	R05605	0.07	0.06	mmol/g*h
F6P + ATP -> 2 T3P		1	0.16	mmol/g*h
2 P5P -> S7P + T3P		0.09	0.02	mmol/g*h
P5P + E4P -> F6P + T3P	R01067	0.07	0.02	mmol/g*h
S7P + T3P -> E4P + F6P	R01827	0.09	0.02	mmol/g*h
T3P -> PGA + ATP + NADH		2.14	0.32	mmol/g*h
PGA -> PEP		2.07	0.33	mmol/g*h
PEP -> PYR + ATP	R00200	2.04	0.34	mmol/g*h
PYR -> AcCoA + CO2 + NADH	R01196	1.98	0.36	mmol/g*h
OAA + AcCoA -> ICT		1.77	0.37	mmol/g*h
ICT -> OGA + CO2 + NADPH	R00267	1.66	0.4	mmol/g*h
OGA -> FUM + CO2 + 1.5 ATP + 2 NADH		1.61	0.41	mmol/g*h
FUM -> MAL	R01082	1.73	0.38	mmol/g*h
MAL -> OAA + NADH	R00342	1.84	0.37	mmol/g*h
MAL -> PYR + CO2 + NADH	R00214	0	0	mmol/g*h
OAA + ATP -> PEP + CO2	R00341	0.66	0.12	mmol/g*h
PEP + CO2 -> OAA	R00341	0.64	0.13	mmol/g*h
AcCoA -> Acetate + ATP	R00229	0	0	mmol/g*h
ICT + AcCoA -> MAL + FUM + NADH		0.11	0.09	mmol/g*h
NADPH -> NADH	R00105	1.45	0.87	mmol/g*h
O2 + 2 NADH -> 2 PO + ATP		5.42	1.17	mmol/g*h
Biomass		0.05	0.01	mmol/g*h

Graph

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Reaction	KEGG ID	Flux	Std	Unit
GLC + ATP -> G6P	R02738	1.15	0.18	mmol/g*h
G6P -> 6PG + NADPH	R01528	0.34	0.07	mmol/g*h
6PG -> P5P + CO2 + NADPH	R10221	0.26	0.07	mmol/g*h
G6P -> F6P	R02740	0.81	0.14	mmol/g*h
6PG -> T3P + PYR	R05605	0.08	0.07	mmol/g*h
F6P + ATP -> 2 T3P		0.95	0.17	mmol/g*h
2 P5P -> S7P + T3P		0.09	0.02	mmol/g*h
P5P + E4P -> F6P + T3P	R01067	0.06	0.02	mmol/g*h
S7P + T3P -> E4P + F6P	R01827	0.09	0.02	mmol/g*h
T3P -> PGA + ATP + NADH		2.04	0.35	mmol/g*h
PGA -> PEP		1.95	0.35	mmol/g*h
PEP -> PYR + ATP	R00200	2.15	0.4	mmol/g*h
PYR -> AcCoA + CO2 + NADH	R01196	2.26	0.46	mmol/g*h
OAA + AcCoA -> ICT		1.53	0.33	mmol/g*h
ICT -> OGA + CO2 + NADPH	R00267	0.92	0.22	mmol/g*h
OGA -> FUM + CO2 + 1.5 ATP + 2 NADH		0.85	0.21	mmol/g*h
FUM -> MAL	R01082	1.46	0.32	mmol/g*h
MAL -> OAA + NADH	R00342	1.87	0.39	mmol/g*h
MAL -> PYR + CO2 + NADH	R00214	0.21	0.07	mmol/g*h
OAA + ATP -> PEP + CO2	R00341	0.61	0.13	mmol/g*h
PEP + CO2 -> OAA	R00341	0.35	0.08	mmol/g*h
AcCoA -> Acetate + ATP	R00229	0	0	mmol/g*h
ICT + AcCoA -> MAL + FUM + NADH		0.61	0.13	mmol/g*h
NADPH -> NADH	R00105	0.53	0.77	mmol/g*h
O2 + 2 NADH -> 2 PO + ATP		4.68	1.07	mmol/g*h
Biomass		0.06	0	mmol/g*h

Graph

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Reaction	KEGG ID	Flux	Std	Unit
GLC + ATP -> G6P	R02738	1.67	0.18	mmol/g*h
G6P -> 6PG + NADPH	R01528	0.42	0.08	mmol/g*h
6PG -> P5P + CO2 + NADPH	R10221	0.31	0.09	mmol/g*h
G6P -> F6P	R02740	1.25	0.16	mmol/g*h
6PG -> T3P + PYR	R05605	0.11	0.11	mmol/g*h
F6P + ATP -> 2 T3P		1.4	0.18	mmol/g*h
2 P5P -> S7P + T3P		0.1	0.03	mmol/g*h
P5P + E4P -> F6P + T3P	R01067	0.06	0.03	mmol/g*h
S7P + T3P -> E4P + F6P	R01827	0.1	0.03	mmol/g*h
T3P -> PGA + ATP + NADH		2.97	0.35	mmol/g*h
PGA -> PEP		2.83	0.36	mmol/g*h
PEP -> PYR + ATP	R00200	3.44	0.45	mmol/g*h
PYR -> AcCoA + CO2 + NADH	R01196	3.24	0.47	mmol/g*h
OAA + AcCoA -> ICT		2.05	0.44	mmol/g*h
ICT -> OGA + CO2 + NADPH	R00267	1.06	0.46	mmol/g*h
OGA -> FUM + CO2 + 1.5 ATP + 2 NADH		0.93	0.49	mmol/g*h
FUM -> MAL	R01082	1.91	0.45	mmol/g*h
MAL -> OAA + NADH	R00342	2.89	0.48	mmol/g*h
MAL -> PYR + CO2 + NADH	R00214	0	0.07	mmol/g*h
OAA + ATP -> PEP + CO2	R00341	1.19	0.17	mmol/g*h
PEP + CO2 -> OAA	R00341	0.5	0.1	mmol/g*h
AcCoA -> Acetate + ATP	R00229	0	0	mmol/g*h
ICT + AcCoA -> MAL + FUM + NADH		0.98	0.18	mmol/g*h
NADPH -> NADH	R00105	0.29	0.91	mmol/g*h
O2 + 2 NADH -> 2 PO + ATP		6.21	1.34	mmol/g*h
Biomass		0.08	0.01	mmol/g*h

Graph

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Reaction	KEGG ID	Flux	Std	Unit
GLC + ATP -> G6P	R02738	1.67	0.18	mmol/g*h
G6P -> 6PG + NADPH	R01528	0.51	0.08	mmol/g*h
6PG -> P5P + CO2 + NADPH	R10221	0.39	0.09	mmol/g*h
G6P -> F6P	R02740	1.16	0.14	mmol/g*h
6PG -> T3P + PYR	R05605	0.12	0.11	mmol/g*h
F6P + ATP -> 2 T3P		1.37	0.18	mmol/g*h
2 P5P -> S7P + T3P		0.13	0.03	mmol/g*h
P5P + E4P -> F6P + T3P	R01067	0.09	0.03	mmol/g*h
S7P + T3P -> E4P + F6P	R01827	0.13	0.03	mmol/g*h
T3P -> PGA + ATP + NADH		2.94	0.35	mmol/g*h
PGA -> PEP		2.8	0.35	mmol/g*h
PEP -> PYR + ATP	R00200	3.37	0.44	mmol/g*h
PYR -> AcCoA + CO2 + NADH	R01196	3.17	0.46	mmol/g*h
OAA + AcCoA -> ICT		2.02	0.43	mmol/g*h
ICT -> OGA + CO2 + NADPH	R00267	1.08	0.46	mmol/g*h
OGA -> FUM + CO2 + 1.5 ATP + 2 NADH		0.94	0.49	mmol/g*h
FUM -> MAL	R01082	1.89	0.45	mmol/g*h
MAL -> OAA + NADH	R00342	2.82	0.47	mmol/g*h
MAL -> PYR + CO2 + NADH	R00214	0	0.06	mmol/g*h
OAA + ATP -> PEP + CO2	R00341	1.17	0.17	mmol/g*h
PEP + CO2 -> OAA	R00341	0.53	0.1	mmol/g*h
AcCoA -> Acetate + ATP	R00229	0	0	mmol/g*h
ICT + AcCoA -> MAL + FUM + NADH		0.94	0.17	mmol/g*h
NADPH -> NADH	R00105	0.48	0.92	mmol/g*h
O2 + 2 NADH -> 2 PO + ATP		6.22	1.34	mmol/g*h
Biomass		0.09	0.01	mmol/g*h

Graph

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Reaction	KEGG ID	Flux	Std	Unit
GLC + ATP -> G6P	R02738	1.8	0.16	mmol/g*h
G6P -> 6PG + NADPH	R01528	0.45	0.08	mmol/g*h
6PG -> P5P + CO2 + NADPH	R10221	0.34	0.09	mmol/g*h
G6P -> F6P	R02740	1.35	0.14	mmol/g*h
6PG -> T3P + PYR	R05605	0.11	0.12	mmol/g*h
F6P + ATP -> 2 T3P		1.52	0.17	mmol/g*h
2 P5P -> S7P + T3P		0.11	0.03	mmol/g*h
P5P + E4P -> F6P + T3P	R01067	0.07	0.03	mmol/g*h
S7P + T3P -> E4P + F6P	R01827	0.11	0.03	mmol/g*h
T3P -> PGA + ATP + NADH		3.21	0.32	mmol/g*h
PGA -> PEP		3.06	0.33	mmol/g*h
PEP -> PYR + ATP	R00200	3.19	0.36	mmol/g*h
PYR -> AcCoA + CO2 + NADH	R01196	3.23	0.33	mmol/g*h
OAA + AcCoA -> ICT		2.2	0.4	mmol/g*h
ICT -> OGA + CO2 + NADPH	R00267	1.4	0.46	mmol/g*h
OGA -> FUM + CO2 + 1.5 ATP + 2 NADH		1.25	0.48	mmol/g*h
FUM -> MAL	R01082	2.05	0.42	mmol/g*h
MAL -> OAA + NADH	R00342	2.59	0.38	mmol/g*h
MAL -> PYR + CO2 + NADH	R00214	0.26	0.13	mmol/g*h
OAA + ATP -> PEP + CO2	R00341	0.86	0.12	mmol/g*h
PEP + CO2 -> OAA	R00341	0.64	0.11	mmol/g*h
AcCoA -> Acetate + ATP	R00229	0	0	mmol/g*h
ICT + AcCoA -> MAL + FUM + NADH		0.8	0.06	mmol/g*h
NADPH -> NADH	R00105	0.56	0.9	mmol/g*h
O2 + 2 NADH -> 2 PO + ATP		6.68	1.23	mmol/g*h
Biomass		0.09	0.01	mmol/g*h

Graph

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Reaction	KEGG ID	Flux	Std	Unit
GLC + ATP -> G6P	R02738	1.8	0.16	mmol/g*h
G6P -> 6PG + NADPH	R01528	0.54	0.08	mmol/g*h
6PG -> P5P + CO2 + NADPH	R10221	0.42	0.09	mmol/g*h
G6P -> F6P	R02740	1.25	0.13	mmol/g*h
6PG -> T3P + PYR	R05605	0.12	0.11	mmol/g*h
F6P + ATP -> 2 T3P		1.48	0.16	mmol/g*h
2 P5P -> S7P + T3P		0.14	0.03	mmol/g*h
P5P + E4P -> F6P + T3P	R01067	0.1	0.03	mmol/g*h
S7P + T3P -> E4P + F6P	R01827	0.14	0.03	mmol/g*h
T3P -> PGA + ATP + NADH		3.17	0.31	mmol/g*h
PGA -> PEP		3.02	0.32	mmol/g*h
PEP -> PYR + ATP	R00200	3.15	0.35	mmol/g*h
PYR -> AcCoA + CO2 + NADH	R01196	3.21	0.41	mmol/g*h
OAA + AcCoA -> ICT		2.17	0.4	mmol/g*h
ICT -> OGA + CO2 + NADPH	R00267	1.36	0.37	mmol/g*h
OGA -> FUM + CO2 + 1.5 ATP + 2 NADH		1.21	0.39	mmol/g*h
FUM -> MAL	R01082	2.02	0.42	mmol/g*h
MAL -> OAA + NADH	R00342	2.55	0.38	mmol/g*h
MAL -> PYR + CO2 + NADH	R00214	0.28	0.16	mmol/g*h
OAA + ATP -> PEP + CO2	R00341	0.85	0.12	mmol/g*h
PEP + CO2 -> OAA	R00341	0.63	0.11	mmol/g*h
AcCoA -> Acetate + ATP	R00229	0	0	mmol/g*h
ICT + AcCoA -> MAL + FUM + NADH		0.81	0.04	mmol/g*h
NADPH -> NADH	R00105	0.71	0.86	mmol/g*h
O2 + 2 NADH -> 2 PO + ATP		6.68	1.23	mmol/g*h
Biomass		0.09	0.01	mmol/g*h

Graph

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Reaction	KEGG ID	Flux	Std	Unit
GLC + ATP -> G6P	R02738	2.11	0.3	mmol/g*h
G6P -> 6PG + NADPH	R01528	0.53	0.1	mmol/g*h
6PG -> P5P + CO2 + NADPH	R10221	0.39	0.11	mmol/g*h
G6P -> F6P	R02740	1.57	0.25	mmol/g*h
6PG -> T3P + PYR	R05605	0.14	0.14	mmol/g*h
F6P + ATP -> 2 T3P		1.77	0.29	mmol/g*h
2 P5P -> S7P + T3P		0.13	0.04	mmol/g*h
P5P + E4P -> F6P + T3P	R01067	0.08	0.04	mmol/g*h
S7P + T3P -> E4P + F6P	R01827	0.13	0.04	mmol/g*h
T3P -> PGA + ATP + NADH		3.74	0.57	mmol/g*h
PGA -> PEP		3.56	0.57	mmol/g*h
PEP -> PYR + ATP	R00200	3.51	0.61	mmol/g*h
PYR -> AcCoA + CO2 + NADH	R01196	3.82	0.72	mmol/g*h
OAA + AcCoA -> ICT		2.59	0.57	mmol/g*h
ICT -> OGA + CO2 + NADPH	R00267	1.63	0.46	mmol/g*h
OGA -> FUM + CO2 + 1.5 ATP + 2 NADH		1.46	0.46	mmol/g*h
FUM -> MAL	R01082	2.43	0.57	mmol/g*h
MAL -> OAA + NADH	R00342	2.84	0.6	mmol/g*h
MAL -> PYR + CO2 + NADH	R00214	0.56	0.14	mmol/g*h
OAA + ATP -> PEP + CO2	R00341	0.72	0.15	mmol/g*h
PEP + CO2 -> OAA	R00341	0.66	0.14	mmol/g*h
AcCoA -> Acetate + ATP	R00229	0	0	mmol/g*h
ICT + AcCoA -> MAL + FUM + NADH		0.97	0.19	mmol/g*h
NADPH -> NADH	R00105	0.55	0.9	mmol/g*h
O2 + 2 NADH -> 2 PO + ATP		7.83	1.78	mmol/g*h
Biomass		0.11	0	mmol/g*h

Graph

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Reaction	KEGG ID	Flux	Std	Unit
GLC + ATP -> G6P	R02738	2.12	0.3	mmol/g*h
G6P -> 6PG + NADPH	R01528	0.65	0.11	mmol/g*h
6PG -> P5P + CO2 + NADPH	R10221	0.5	0.12	mmol/g*h
G6P -> F6P	R02740	1.46	0.23	mmol/g*h
6PG -> T3P + PYR	R05605	0.15	0.13	mmol/g*h
F6P + ATP -> 2 T3P		1.73	0.28	mmol/g*h
2 P5P -> S7P + T3P		0.16	0.04	mmol/g*h
P5P + E4P -> F6P + T3P	R01067	0.11	0.04	mmol/g*h
S7P + T3P -> E4P + F6P	R01827	0.16	0.04	mmol/g*h
T3P -> PGA + ATP + NADH		3.7	0.56	mmol/g*h
PGA -> PEP		3.52	0.56	mmol/g*h
PEP -> PYR + ATP	R00200	3.47	0.6	mmol/g*h
PYR -> AcCoA + CO2 + NADH	R01196	3.79	0.71	mmol/g*h
OAA + AcCoA -> ICT		2.57	0.57	mmol/g*h
ICT -> OGA + CO2 + NADPH	R00267	1.61	0.46	mmol/g*h
OGA -> FUM + CO2 + 1.5 ATP + 2 NADH		1.45	0.46	mmol/g*h
FUM -> MAL	R01082	2.41	0.57	mmol/g*h
MAL -> OAA + NADH	R00342	2.82	0.6	mmol/g*h
MAL -> PYR + CO2 + NADH	R00214	0.55	0.14	mmol/g*h
OAA + ATP -> PEP + CO2	R00341	0.71	0.15	mmol/g*h
PEP + CO2 -> OAA	R00341	0.65	0.14	mmol/g*h
AcCoA -> Acetate + ATP	R00229	0	0	mmol/g*h
ICT + AcCoA -> MAL + FUM + NADH		0.96	0.19	mmol/g*h
NADPH -> NADH	R00105	0.76	0.92	mmol/g*h
O2 + 2 NADH -> 2 PO + ATP		7.87	1.77	mmol/g*h
Biomass		0.11	0	mmol/g*h

Graph

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Reaction	KEGG ID	Flux	Std	Unit
GLC + ATP -> G6P	R02738	2.84	0.15	mmol/g*h
G6P -> 6PG + NADPH	R01528	1.24	0.11	mmol/g*h
6PG -> P5P + CO2 + NADPH	R10221	0.97	0.14	mmol/g*h
G6P -> F6P	R02740	1.58	0.13	mmol/g*h
6PG -> T3P + PYR	R05605	0.28	0.14	mmol/g*h
F6P + ATP -> 2 T3P		2.12	0.17	mmol/g*h
2 P5P -> S7P + T3P		0.31	0.05	mmol/g*h
P5P + E4P -> F6P + T3P	R01067	0.23	0.05	mmol/g*h
S7P + T3P -> E4P + F6P	R01827	0.31	0.05	mmol/g*h
T3P -> PGA + ATP + NADH		4.72	0.3	mmol/g*h
PGA -> PEP		4.42	0.3	mmol/g*h
PEP -> PYR + ATP	R00200	4.58	0.35	mmol/g*h
PYR -> AcCoA + CO2 + NADH	R01196	4.64	0.41	mmol/g*h
OAA + AcCoA -> ICT		2.88	0.31	mmol/g*h
ICT -> OGA + CO2 + NADPH	R00267	1.56	0.24	mmol/g*h
OGA -> FUM + CO2 + 1.5 ATP + 2 NADH		1.28	0.25	mmol/g*h
FUM -> MAL	R01082	2.61	0.32	mmol/g*h
MAL -> OAA + NADH	R00342	3.52	0.35	mmol/g*h
MAL -> PYR + CO2 + NADH	R00214	0.41	0.12	mmol/g*h
OAA + ATP -> PEP + CO2	R00341	1.16	0.14	mmol/g*h
PEP + CO2 -> OAA	R00341	0.84	0.1	mmol/g*h
AcCoA -> Acetate + ATP	R00229	0	0	mmol/g*h
ICT + AcCoA -> MAL + FUM + NADH		1.33	0.14	mmol/g*h
NADPH -> NADH	R00105	0.58	0.8	mmol/g*h
O2 + 2 NADH -> 2 PO + ATP		9.11	1.01	mmol/g*h
Biomass		0.18	0.01	mmol/g*h

Graph

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Reaction	KEGG ID	Flux	Std	Unit
GLC + ATP -> G6P	R02738	2.84	0.15	mmol/g*h
G6P -> 6PG + NADPH	R01528	1.28	0.11	mmol/g*h
6PG -> P5P + CO2 + NADPH	R10221	0.97	0.13	mmol/g*h
G6P -> F6P	R02740	1.55	0.12	mmol/g*h
6PG -> T3P + PYR	R05605	0.31	0.14	mmol/g*h
F6P + ATP -> 2 T3P		2.08	0.16	mmol/g*h
2 P5P -> S7P + T3P		0.31	0.05	mmol/g*h
P5P + E4P -> F6P + T3P	R01067	0.23	0.05	mmol/g*h
S7P + T3P -> E4P + F6P	R01827	0.31	0.05	mmol/g*h
T3P -> PGA + ATP + NADH		4.68	0.3	mmol/g*h
PGA -> PEP		4.38	0.3	mmol/g*h
PEP -> PYR + ATP	R00200	4.53	0.35	mmol/g*h
PYR -> AcCoA + CO2 + NADH	R01196	4.63	0.41	mmol/g*h
OAA + AcCoA -> ICT		2.88	0.31	mmol/g*h
ICT -> OGA + CO2 + NADPH	R00267	1.56	0.24	mmol/g*h
OGA -> FUM + CO2 + 1.5 ATP + 2 NADH		1.28	0.25	mmol/g*h
FUM -> MAL	R01082	2.6	0.32	mmol/g*h
MAL -> OAA + NADH	R00342	3.51	0.35	mmol/g*h
MAL -> PYR + CO2 + NADH	R00214	0.41	0.12	mmol/g*h
OAA + ATP -> PEP + CO2	R00341	1.16	0.13	mmol/g*h
PEP + CO2 -> OAA	R00341	0.83	0.1	mmol/g*h
AcCoA -> Acetate + ATP	R00229	0	0	mmol/g*h
ICT + AcCoA -> MAL + FUM + NADH		1.32	0.14	mmol/g*h
NADPH -> NADH	R00105	0.61	0.8	mmol/g*h
O2 + 2 NADH -> 2 PO + ATP		9.09	1.01	mmol/g*h
Biomass		0.18	0.01	mmol/g*h

Graph

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Reaction	KEGG ID	Flux	Std	Unit
GLC + ATP -> G6P	R02738	4.31	0.25	mmol/g*h
G6P -> 6PG + NADPH	R01528	1.61	0.16	mmol/g*h
6PG -> P5P + CO2 + NADPH	R10221	1.23	0.18	mmol/g*h
G6P -> F6P	R02740	2.68	0.21	mmol/g*h
6PG -> T3P + PYR	R05605	0.38	0.19	mmol/g*h
F6P + ATP -> 2 T3P		3.31	0.26	mmol/g*h
2 P5P -> S7P + T3P		0.39	0.06	mmol/g*h
P5P + E4P -> F6P + T3P	R01067	0.27	0.06	mmol/g*h
S7P + T3P -> E4P + F6P	R01827	0.39	0.06	mmol/g*h
T3P -> PGA + ATP + NADH		7.24	0.49	mmol/g*h
PGA -> PEP		6.77	0.49	mmol/g*h
PEP -> PYR + ATP	R00200	5.48	0.43	mmol/g*h
PYR -> AcCoA + CO2 + NADH	R01196	5.57	0.5	mmol/g*h
OAA + AcCoA -> ICT		4.35	0.51	mmol/g*h
ICT -> OGA + CO2 + NADPH	R00267	3.81	0.56	mmol/g*h
OGA -> FUM + CO2 + 1.5 ATP + 2 NADH		3.4	0.57	mmol/g*h
FUM -> MAL	R01082	3.94	0.51	mmol/g*h
MAL -> OAA + NADH	R00342	3.81	0.43	mmol/g*h
MAL -> PYR + CO2 + NADH	R00214	0.67	0.13	mmol/g*h
OAA + ATP -> PEP + CO2	R00341	0.64	0.12	mmol/g*h
PEP + CO2 -> OAA	R00341	1.68	0.19	mmol/g*h
AcCoA -> Acetate + ATP	R00229	0	0	mmol/g*h
ICT + AcCoA -> MAL + FUM + NADH		0.54	0.17	mmol/g*h
NADPH -> NADH	R00105	1.82	1.01	mmol/g*h
O2 + 2 NADH -> 2 PO + ATP		13.58	1.59	mmol/g*h
Biomass		0.28	0.01	mmol/g*h

Graph

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Reaction	KEGG ID	Flux	Std	Unit
GLC + ATP -> G6P	R02738	4.31	0.25	mmol/g*h
G6P -> 6PG + NADPH	R01528	1.67	0.16	mmol/g*h
6PG -> P5P + CO2 + NADPH	R10221	1.29	0.18	mmol/g*h
G6P -> F6P	R02740	2.63	0.21	mmol/g*h
6PG -> T3P + PYR	R05605	0.38	0.19	mmol/g*h
F6P + ATP -> 2 T3P		3.3	0.26	mmol/g*h
2 P5P -> S7P + T3P		0.41	0.06	mmol/g*h
P5P + E4P -> F6P + T3P	R01067	0.29	0.06	mmol/g*h
S7P + T3P -> E4P + F6P	R01827	0.41	0.06	mmol/g*h
T3P -> PGA + ATP + NADH		7.23	0.49	mmol/g*h
PGA -> PEP		6.76	0.49	mmol/g*h
PEP -> PYR + ATP	R00200	5.47	0.43	mmol/g*h
PYR -> AcCoA + CO2 + NADH	R01196	5.55	0.5	mmol/g*h
OAA + AcCoA -> ICT		4.34	0.51	mmol/g*h
ICT -> OGA + CO2 + NADPH	R00267	3.8	0.56	mmol/g*h
OGA -> FUM + CO2 + 1.5 ATP + 2 NADH		3.38	0.57	mmol/g*h
FUM -> MAL	R01082	3.92	0.51	mmol/g*h
MAL -> OAA + NADH	R00342	3.8	0.43	mmol/g*h
MAL -> PYR + CO2 + NADH	R00214	0.67	0.13	mmol/g*h
OAA + ATP -> PEP + CO2	R00341	0.64	0.12	mmol/g*h
PEP + CO2 -> OAA	R00341	1.67	0.19	mmol/g*h
AcCoA -> Acetate + ATP	R00229	0	0	mmol/g*h
ICT + AcCoA -> MAL + FUM + NADH		0.54	0.17	mmol/g*h
NADPH -> NADH	R00105	1.93	1.01	mmol/g*h
O2 + 2 NADH -> 2 PO + ATP		13.6	1.59	mmol/g*h
Biomass		0.28	0.01	mmol/g*h

Graph

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Reaction	KEGG ID	Flux	Std	Unit
GLC + ATP -> G6P	R02738	4.62	0.29	mmol/g*h
G6P -> 6PG + NADPH	R01528	1.74	0.17	mmol/g*h
6PG -> P5P + CO2 + NADPH	R10221	1.33	0.2	mmol/g*h
G6P -> F6P	R02740	2.86	0.24	mmol/g*h
6PG -> T3P + PYR	R05605	0.42	0.21	mmol/g*h
F6P + ATP -> 2 T3P		3.53	0.29	mmol/g*h
2 P5P -> S7P + T3P		0.41	0.07	mmol/g*h
P5P + E4P -> F6P + T3P	R01067	0.28	0.07	mmol/g*h
S7P + T3P -> E4P + F6P	R01827	0.41	0.07	mmol/g*h
T3P -> PGA + ATP + NADH		7.73	0.55	mmol/g*h
PGA -> PEP		7.2	0.55	mmol/g*h
PEP -> PYR + ATP	R00200	6.85	0.57	mmol/g*h
PYR -> AcCoA + CO2 + NADH	R01196	6.2	0.56	mmol/g*h
OAA + AcCoA -> ICT		4.52	0.56	mmol/g*h
ICT -> OGA + CO2 + NADPH	R00267	3.58	0.62	mmol/g*h
OGA -> FUM + CO2 + 1.5 ATP + 2 NADH		3.11	0.63	mmol/g*h
FUM -> MAL	R01082	4.05	0.57	mmol/g*h
MAL -> OAA + NADH	R00342	4.99	0.56	mmol/g*h
MAL -> PYR + CO2 + NADH	R00214	0	0.01	mmol/g*h
OAA + ATP -> PEP + CO2	R00341	1.83	0.21	mmol/g*h
PEP + CO2 -> OAA	R00341	1.9	0.22	mmol/g*h
AcCoA -> Acetate + ATP	R00229	0	0	mmol/g*h
ICT + AcCoA -> MAL + FUM + NADH		0.94	0.17	mmol/g*h
NADPH -> NADH	R00105	1.34	1.06	mmol/g*h
O2 + 2 NADH -> 2 PO + ATP		14.11	1.77	mmol/g*h
Biomass		0.31	0.01	mmol/g*h

Graph

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Reaction	KEGG ID	Flux	Std	Unit
GLC + ATP -> G6P	R02738	4.62	0.29	mmol/g*h
G6P -> 6PG + NADPH	R01528	1.8	0.17	mmol/g*h
6PG -> P5P + CO2 + NADPH	R10221	1.39	0.2	mmol/g*h
G6P -> F6P	R02740	2.8	0.23	mmol/g*h
6PG -> T3P + PYR	R05605	0.42	0.2	mmol/g*h
F6P + ATP -> 2 T3P		3.52	0.29	mmol/g*h
2 P5P -> S7P + T3P		0.43	0.07	mmol/g*h
P5P + E4P -> F6P + T3P	R01067	0.3	0.07	mmol/g*h
S7P + T3P -> E4P + F6P	R01827	0.43	0.07	mmol/g*h
T3P -> PGA + ATP + NADH		7.71	0.55	mmol/g*h
PGA -> PEP		7.19	0.55	mmol/g*h
PEP -> PYR + ATP	R00200	6.84	0.56	mmol/g*h
PYR -> AcCoA + CO2 + NADH	R01196	6.19	0.56	mmol/g*h
OAA + AcCoA -> ICT		4.5	0.56	mmol/g*h
ICT -> OGA + CO2 + NADPH	R00267	3.56	0.62	mmol/g*h
OGA -> FUM + CO2 + 1.5 ATP + 2 NADH		3.1	0.63	mmol/g*h
FUM -> MAL	R01082	4.04	0.57	mmol/g*h
MAL -> OAA + NADH	R00342	4.98	0.56	mmol/g*h
MAL -> PYR + CO2 + NADH	R00214	0	0.01	mmol/g*h
OAA + ATP -> PEP + CO2	R00341	1.82	0.21	mmol/g*h
PEP + CO2 -> OAA	R00341	1.89	0.22	mmol/g*h
AcCoA -> Acetate + ATP	R00229	0	0	mmol/g*h
ICT + AcCoA -> MAL + FUM + NADH		0.94	0.17	mmol/g*h
NADPH -> NADH	R00105	1.45	1.07	mmol/g*h
O2 + 2 NADH -> 2 PO + ATP		14.14	1.77	mmol/g*h
Biomass		0.31	0.01	mmol/g*h

Graph

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Reaction	KEGG ID	Flux	Std	Unit
GLC + ATP -> G6P	R02738	5.71	0.28	mmol/g*h
G6P -> 6PG + NADPH	R01528	2.06	0.19	mmol/g*h
6PG -> P5P + CO2 + NADPH	R10221	1.65	0.18	mmol/g*h
G6P -> F6P	R02740	3.63	0.25	mmol/g*h
6PG -> T3P + PYR	R05605	0.41	0.21	mmol/g*h
F6P + ATP -> 2 T3P		4.44	0.29	mmol/g*h
2 P5P -> S7P + T3P		0.5	0.06	mmol/g*h
P5P + E4P -> F6P + T3P	R01067	0.34	0.06	mmol/g*h
S7P + T3P -> E4P + F6P	R01827	0.5	0.06	mmol/g*h
T3P -> PGA + ATP + NADH		9.59	0.54	mmol/g*h
PGA -> PEP		8.91	0.54	mmol/g*h
PEP -> PYR + ATP	R00200	6.86	0.49	mmol/g*h
PYR -> AcCoA + CO2 + NADH	R01196	6.42	0.53	mmol/g*h
OAA + AcCoA -> ICT		5.48	0.53	mmol/g*h
ICT -> OGA + CO2 + NADPH	R00267	5.48	0.53	mmol/g*h
OGA -> FUM + CO2 + 1.5 ATP + 2 NADH		4.91	0.53	mmol/g*h
FUM -> MAL	R01082	4.91	0.53	mmol/g*h
MAL -> OAA + NADH	R00342	4.45	0.47	mmol/g*h
MAL -> PYR + CO2 + NADH	R00214	0.46	0.13	mmol/g*h
OAA + ATP -> PEP + CO2	R00341	0.88	0.15	mmol/g*h
PEP + CO2 -> OAA	R00341	2.59	0.22	mmol/g*h
AcCoA -> Acetate + ATP	R00229	0	0	mmol/g*h
ICT + AcCoA -> MAL + FUM + NADH		0	0	mmol/g*h
NADPH -> NADH	R00105	2.58	1	mmol/g*h
O2 + 2 NADH -> 2 PO + ATP		17.19	1.66	mmol/g*h
Biomass		0.4	0.01	mmol/g*h

Graph

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Reaction	KEGG ID	Flux	Std	Unit
GLC + ATP -> G6P	R02738	5.84	0.33	mmol/g*h
G6P -> 6PG + NADPH	R01528	2.09	0.2	mmol/g*h
6PG -> P5P + CO2 + NADPH	R10221	1.63	0.19	mmol/g*h
G6P -> F6P	R02740	3.73	0.28	mmol/g*h
6PG -> T3P + PYR	R05605	0.46	0.22	mmol/g*h
F6P + ATP -> 2 T3P		4.53	0.33	mmol/g*h
2 P5P -> S7P + T3P		0.49	0.06	mmol/g*h
P5P + E4P -> F6P + T3P	R01067	0.33	0.06	mmol/g*h
S7P + T3P -> E4P + F6P	R01827	0.49	0.06	mmol/g*h
T3P -> PGA + ATP + NADH		9.79	0.63	mmol/g*h
PGA -> PEP		9.09	0.64	mmol/g*h
PEP -> PYR + ATP	R00200	7.03	0.51	mmol/g*h
PYR -> AcCoA + CO2 + NADH	R01196	6.55	0.7	mmol/g*h
OAA + AcCoA -> ICT		5.58	0.72	mmol/g*h
ICT -> OGA + CO2 + NADPH	R00267	5.58	0.72	mmol/g*h
OGA -> FUM + CO2 + 1.5 ATP + 2 NADH		5	0.74	mmol/g*h
FUM -> MAL	R01082	5	0.74	mmol/g*h
MAL -> OAA + NADH	R00342	4.58	0.53	mmol/g*h
MAL -> PYR + CO2 + NADH	R00214	0.42	0.34	mmol/g*h
OAA + ATP -> PEP + CO2	R00341	0.9	0.16	mmol/g*h
PEP + CO2 -> OAA	R00341	2.61	0.34	mmol/g*h
AcCoA -> Acetate + ATP	R00229	0	0	mmol/g*h
ICT + AcCoA -> MAL + FUM + NADH		0	0	mmol/g*h
NADPH -> NADH	R00105	2.51	1.23	mmol/g*h
O2 + 2 NADH -> 2 PO + ATP		17.47	2.24	mmol/g*h
Biomass		0.41	0.02	mmol/g*h

Graph

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Manuscript title	Nonlinear dependency of intracellular fluxes on growth rate in miniaturized continuous cultures of Escherichia coli.
PubMed ID	16461663
Journal	Applied and Environmental Microbiology
Year	2006
Authors	Annik Nanchen, Alexander Schicker and Uwe Sauer
Affiliations	Institute of Molecular Systems Biology, ETH Zurich, Zurich, Switzerland
Keywords	Escherichia coli, continuous culture, intracellular fluxes
Full text article	Nanchen_2006.pdf
Project name	not specified

Organism	Escherichia coli
Strain	MG1655
Data type	flux measurements
Data units	(mmol/gDW.h)
Execution date	not specified

APA	KiMoSys (https://kimosys.org). (2020, December 22). Data EntryID 65 (Escherichia coli). https://doi.org/10.34619/5n39-r321
MLA	KiMoSys (https://kimosys.org). "Data EntryID 65 (Escherichia coli)." 2024, https://doi.org/10.34619/5n39-r321
ISO-690	KiMoSys (https://kimosys.org). Data EntryID 65 (Escherichia coli). [online], [Accessed 22 December 2024]. Available from: https://doi.org/10.34619/5n39-r321
BibTeX	@misc{ KiMoSysDataEntryID65, author = "{KiMoSys (https://kimosys.org)}", title = "Data EntryID 65 (Escherichia coli)", url = https://doi.org/10.34619/5n39-r321", doi = "10.34619/5n39-r321", note = "[Online; accessed 22-December-2024]" }

Detail View - Data AccessID 65

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