Minimal salts media with 10 mM NH4Cl and 0.4 % glucose.

Detailed protocols for preparing filter cultures and extracting metabolites have been published [1,2].
Metabolite measurement - Cell extracts were analyzed by LC-electrospray ionization (ESI)-MS/MS on a Thermo Quantum triple quadrupole mass spectrometer operating in se ... lected reaction monitoring (SRM) mode. All samples were analyzed within 24 h of their preparation. Separate LC runs were conducted for positive and negative ionization modes, using hydrophilic interaction chromatography and ion-pairing reversed phase chromatography, respectively [3].
Amino acids were derivatized before their quantitation by LC-ESIMS/MS. Cell extract (200 µl) was mixed with triethylamine (5 µl) and benzyl chloral formate (1 µl) to convert amines to N-benzylcarbamate (Cbz) derivatives [4].
As internal standard signals did not vary substantially or systematically between samples within a given time course, the reported metabolite concentration changes are between-sample ratios of the peak heights of the SRM chromatograms without correction for the internal standard response, except for glutamate and glutamine, in which correction for internal standards was performed to maximize quantitative accuracy.

-----------References-------------
[1] Bennett B, Yuan J, Kimball E, Rabinowitz J (2008) Absolute quantitation of intracellular metabolite concentrations by an isotope ratio-based approach. Nat Protoc 3: 1299–1311. http://doi.org/dx4wxn
[2] Yuan J, Bennett B, Rabinowitz J (2008) Kinetic flux profiling for quantitation of cellular metabolic fluxes. Nature Protoc 3: 1328–1340. http://doi.org/cprs6m
[3] Lu W, Bennett BD, Rabinowitz JD (2008). Analytical strategies for LC-MS-based targeted metabolomics. J Chromatogr B Analyt Technol Biomed Life Sci 871: 236–242. http://doi.org/bc7n2m
[4] Kraml CM, Zhou D, Byrne N, McConnell O (2005) Enhanced chromatographic resolution of amine enantiomers as carbobenzyloxy derivatives in high-performance liquid chromatography and supercritical fluid chromatography. J Chromatogr A 1100: 108–115. http://doi.org/fwnt9q