Repository » Data AccessID 52
Detail View - Data AccessID 52

Back

General Information
Manuscript title Metabolic profiling of Escherichia coli cultivations: evaluation of extraction and metabolite analysis procedures.
PubMed ID 17479221
Journal Biotechnology Letters
Year 2007
Authors Julia Hiller, Ezequiel Franco-Lara, Dirk Weuster-Botz
Affiliations Institut fuer Bioverfahrenstechnik, Technische Universitaet Braunschweig, Gausstrasse 17, 38106 Braunschwieg, Germany
Keywords extraction methods, metabolic profiling and multi-substrate
Full text article no file uploaded
Project name not specified


Experiment Description
Organism Escherichia coli
Strain K-12 DSM 498
Data type metabolites at steady-state
Data units mM
Execution date not specified


Experimental Details
Temperature (°C) 37.0
pH 7.0
Carbon source glucose,
Culture mode chemostat
Process condition aerobic
Dilution rate (h⁻¹) 0.125
Working volume (L) 3.0
Biomass concentration (g/L) not specified
Medium composition

Defined medium: 30 g/l glucose, 0.2 g/l NH4Cl, 2g/l (NH4)2 SO4, 3.25 g/l KH2PO4, 2.5 g/l K2HPO4, 1.5 g/l NaH2PO4.H2O, 1 g/l, MgSO4.7H2O, 10 mg/l, CaCl2.2H2O, 0.5 mg/l, ZnSO47H2O, 0.25 mg/l CuCl2.2H2O, 2.5mg/l MnSO4.H2O, 1.75 mg/l CoCl2.6H2O, 0.125 mg/l H3BO3, 2.5 mg/l AlCl3.6H2O, 0.5mg/l Na2MoO4.2H2O, 18.3 mg/l, FeSO4.7H2O. Magnesium and trace-element solution.

General protocol information Sampling method: 6g cell suspension, 25 g 60% MeOH-H2O 30mM TEA, pH=7.5, T=40ºC

Quenching procedure: centrifugation at -19 ºC, 6000g, 3min

Extraction technique: freezing-thawing in methanol

Sample analyzing method: enzymatic, LC-MS

Methods description - Notes

Enzymatic assays - analyses of G6P, F6P, DHAP, GAP, FBP, PEP and PYR were performed by enzymatic assays according to Bergmeyer [1] modified for analysis with a microtiter plate photometer.
LC-MS analysis - analyses of AMP, cAMP, ADP, ATP, NAD, NADP, FAD and acetylcoenz ...

-------------References----------------
[1] Bergmeyer H (1985). Methods of enzymatic analysis, 3rd edn. Verlag Chemie, Weinheim. http://doi.org/bg6tff
[2] Buchholz A, Takors R, Wandrey C (2001). Quantification of intracellular metabolites in Escherichia coli K12 using liquid chromatographic-electrospray ionization tandem mass spectrometric techniques. Anal Biochem 295:129–137. http://doi.org/chsch7

Read more
Data file
KIMODATAID52_v0.xlsx Preview file
Alternative format(s)
KIMODATAID52_metab.txt
KIMODATAID52_metab_compound_SBtab.csv Preview
Export metadata
RDF: metadataDataEntryID52.rdf
XML: metadataDataEntryID52.xml
Plain text: metadataDataEntryID52.txt
Share | Cite Data EntryID 52


Related Data: AccessID 30 | AccessID 35 | AccessID 38 | AccessID 41 | AccessID 44 | AccessID 51 | AccessID 54 | AccessID 63 | AccessID 64 | AccessID 65 | AccessID 67 | AccessID 68 | AccessID 74 | AccessID 75 | AccessID 78 | AccessID 79 | AccessID 80 | AccessID 86 | AccessID 87 | AccessID 92 | AccessID 96 | AccessID 101 | AccessID 102 | AccessID 103 | AccessID 104 | AccessID 105 | AccessID 106 | AccessID 107 | AccessID 108 | AccessID 109 | AccessID 110 | AccessID 112 | AccessID 116 | AccessID 118 | AccessID 119 | AccessID 125 | AccessID 126


Submission and curation

Entered by: Administrator KiMoSysFirst name: Administrator
Affiliation: INESC-ID/IST
Homepage: http://kdbio.inesc-id.pt/kimosys
Interests: mathematical modeling, accessible data, use of data

Created: 2013-05-23 12:57:59 UTC

Updated: 2020-04-24 16:10:31 UTC

Version: 0

Status: (reviewed) 2013-12-06 17:17:38 UTC

Views: 8

Downloads: 0




Associated Models

Here we can find relevant models associated with Data EntryID 52:

Model
EntryID
Model name Category Model Type Data used for Access Json



Associate models to data

- Several models can be associated.

New Model



Back | Top