DataEntryID 78
    General information
      Manuscript title: Metabolic flux analysis in a nonstationary system: fed-batch fermentation of a high yielding strain of E. coli producing 1,3-propanediol.
      PubMed ID: http://www.ncbi.nlm.nih.gov/pubmed/17400499
      Journal: Metabolic Engineering
      Year: 2007
      Authors: Maciek R. Antoniewicz, David F. Kraynie, Lisa A. Laffend, Joanna González-Lergier, Joanne K. Kelleher and Gregory Stephanopoulos
      Affiliations: Department of Chemical Engineering, Bioinformatics and Metabolic Engineering Laboratory, Massachusetts Institute of Technology, Cambridge
      Keywords: E. coli, extracelullar metabolites, HPLC
      Full text article: https://kimosys.org/rails/active_storage/blobs/eyJfcmFpbHMiOnsibWVzc2FnZSI6IkJBaHBBcDBFIiwiZXhwIjpudWxsLCJwdXIiOiJibG9iX2lkIn19--6e6ed57dfd21832003ad33c63a5646a06b16d464/Antoniewicz_2007.pdf
      Project name: not specified

    Experiment description
      Organism: Escherichia coli
      Strain: K-12 overproduce 1,3-propanediol
      Data type: time-series data of metabolites
      Data units: mM
      Execution date: not specified

    Experimental details
      Temperature (°C): 34.0
      pH: 6.8 ± 0.04
      Carbon source: glucose
      Culture mode: fed-batch
      Process condition: aerobic
      Dilution rate (h⁻¹): —
      Working volume: 1.0 L
      Biomass concentration (g/L): 3.0 
      Medium composition: Defined  medium contained (per liter): 2.8g KH2PO4, 2.0 g citric acid monohydrate, 1.1 g MgSO4·7H2O, 0.2 g FeSO4·7H2O, 0.2 g CaCl2·2H2O, 0.5 g Yeast Extract (BBL), 1 mL Balch’s Modified Trace Metals (100x), 0.5 mL sulfuric acid (98%), 1.5 mL phosphoric acid (85%), 0.4 mL Mazu DF204 antifoam, appropriate vitamin B12, and 2 mL spectinomycin (50 mg/mL). 
      General protocol information: Sampling Method: Periodically taken during the fed-batch ; Quenching: —; Extraction list: not used; Analysis list: HPLC-UV/RI;
      Methods description: Concentrations of glucose, glycerol, 1,3-propanediol (PDO), acetate, citrate, and pyruvate in the medium samples were measured by high-performance liquid chromatography (Waters HPLC, Shodex SH1011 sugar column, RI detector).
      Data file: http://kimosys.org/repository/78/download?parameter=1180; 
      Alternative formats: http://kimosys.org/repository/78/attached_files/103/download; 

    Submission and curation
      Entered by: Administrator KiMoSys
      Created: 2013-07-05 14:28:29 UTC
      Updated: 2020-04-24 16:10:34 UTC
      Version: 0
      Status: (reviewed) 2013-12-06 17:17:38 UTC
      Views: 218
      Downloads: 62