DataEntryID 30
    General information
      Manuscript title: Dynamic modeling of the central carbon metabolism of Escherichia coli.
      PubMed ID: http://www.ncbi.nlm.nih.gov/pubmed/17590932
      Journal: Biotechnology and Bioengineering
      Year: 2002
      Authors: Christophe Chassagnole, Naruemol Noisommit-Rizzi, Joachim W. Schmid, Klaus Mauch, Matthias Reuss
      Affiliations: Institute of Biochemical Engineering, University of Stuttgart
      Keywords: dynamic model, Escherichia coli, intracellular metabolites, transient conditions, control and stability analysis
      Full text article: https://kimosys.org/rails/active_storage/blobs/eyJfcmFpbHMiOnsibWVzc2FnZSI6IkJBaHBBbVFFIiwiZXhwIjpudWxsLCJwdXIiOiJibG9iX2lkIn19--04aba72539615dff3c3c14f2a799b51988439bb8/Chassagnole_2002.pdf
      Project name: not specified

    Experiment description
      Organism: Escherichia coli
      Strain: K-12 W3110
      Data type: time-series data of metabolites
      Data units: mM
      Execution date: not specified

    Experimental details
      Temperature (°C): 35.0
      pH: 7.0
      Carbon source: glucose
      Culture mode: chemostat
      Process condition: aerobic
      Dilution rate (h⁻¹): 0.1
      Working volume: 1.5 L
      Biomass concentration (g/L): 8.7
      Medium composition: Synthetic mineral medium: 4.0 g/L Na2SO4, 5.36 g/L (NH4)2SO4, 1.0 g/L NHCl, 7.3 g/L K2HPO4, 1.8 g/L NaH2PO4 H2O, 120 g/L (NH4)2-H-citrate, 4.0 mL/L MgSO4 (1M), 6.0 ml/L trace element solution, 0.02 g/L thiamine, 20.0 g/L glucose.
      General protocol information: Sampling Method: manually every 3 seconds with vacuum-sealed, precooled glass tubes containing the quenching solution; Quenching: liquid nitrogen (-196 ºC) and perchloric acid; Extraction list: perchloric acid; Analysis list: enzymatic, HPLC/HIC;
      Methods description: The concentrations of the extracellular metabolites (glucose, acetate) and most of the intracellular metabolites were determined as describe previously [1]. Improved analysis of the adenine nucleotides was summarized by Mailinger et al. [2] and Meyer et al. [3]. The metabolites and cometabolites from the pentose-phosphate pathway (6PG, NADP+, and NADPH) were measured according to Vaseghi et al. [4].
See also Figure 1 of the original model.

-------------References------------
[1] Theobals U., Mailinger W., Baltes M., Rizzi M., Reuss M., 1997. In vivo analysis of metabolic dynamics in Saccharomyces cerevisae: I. Experimental observations. Biotechnol Bioeng. 55: 305-316. http://doi.org/crtbrx
[2] Mailinger W., Baumeister A., Reuss M., Rizzi M., 1998. Rapid and highly automated determination of adenine and pyridine nucleotides in extracts of Saccharomyces cerevisae using a micro robotic sample preparation-HPLC system. J. Biotechnol. 63: 155-166. http://doi.org/bvbfcn
[3] Meyer S., Noisommit-Rizzi N., Reuss M., Neubauer P., 1999. Optimized analysis of intracellular adenosine and guanosine phosphate in Escherichia coli. Anal Biochem. 271: 43-52. http://doi.org/c7nb99
[4] Vaseghi S., Baumeister A., Rizzi M., Reuss M., 1999. In vivo dynamics of the pentose phosphate pathway in Saccharomyces cerevisae. Metab Eng. 1: 128-140. http://doi.org/bvvrdg 
      Data file: http://kimosys.org/repository/30/download?parameter=1123; 
      Alternative formats: http://kimosys.org/repository/30/attached_files/125/download; http://kimosys.org/repository/30/attached_files/5/download; 

    Submission and curation
      Entered by: Administrator KiMoSys
      Created: 2013-03-06 18:00:37 UTC
      Updated: 2020-04-24 16:10:28 UTC
      Version: 1
      Status: (reviewed) 2013-12-06 17:17:38 UTC
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