DataEntryID 109
    General information
      Manuscript title: Metabolic flux analysis for a ppc mutant Escherichia coli based on 13C-labelling experiments together with enzyme activity assays and intracellular metabolite measurements.
      PubMed ID: http://www.ncbi.nlm.nih.gov/pubmed/15158257
      Journal: FEMS Microbiology Letters
      Year: 2004
      Authors: Lifeng Peng, Marcos J. Arauzo-Bravo, Kazuyuki Shimizu
      Affiliations: Department of Biochemical Engineering and Science, Kyushu Institute of Technology, Iizuka, Fukuoka 820-8502, Japan.
      Keywords: ppc mutant Escherichia coli, 2-Dimensional nuclear magnetic resonance, Gas chromatography-mass spectrometry, Metabolic flux analysis
      Full text article: https://kimosys.org/rails/active_storage/blobs/eyJfcmFpbHMiOnsibWVzc2FnZSI6IkJBaHBBdEFFIiwiZXhwIjpudWxsLCJwdXIiOiJibG9iX2lkIn19--656aa0ad91842c8dec4484f95582d0d39250e60d/Peng_2004.pdf
      Project name: not specified

    Experiment description
      Organism: Escherichia coli
      Strain: BW25113 and ppc mutant
      Data type: enzyme/protein concentrations
      Data units: µmol/min mg(protein)
      Execution date: not specified

    Experimental details
      Temperature (°C): 37
      pH: 7.0
      Carbon source: glucose
      Culture mode: chemostat
      Process condition: aerobic
      Dilution rate (h⁻¹): 0.2
      Working volume: 1.0 L
      Biomass concentration (g/L): Yield of biomass on glucose (g/g): 0,48 (WT) and 0,55 (ppc)
      Medium composition: M9 minimal medium as described previously [1].
      General protocol information: Measurement method: 2D gel-MALDI-TOF;
      Methods description: Enzyme activities were measured spectrophotometrical. Further details on preparation of cell-free extracts and assay procedures for enzyme activities can been seen in [2].
------------------------------------References----------------------------------
[1] Zhao, J. and Shimizu, K. (2002). J. Biotechnol. 101, 101–117. http://doi.org/cdjhrn
[2] Peng, L. and Shimizu, K. (2003) . Appl. Microbiol. Biotechnol. 61, 163–178. http://doi.org/dt3mcm
      Data file: http://kimosys.org/repository/109/download?parameter=1231; 
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    Submission and curation
      Entered by: Administrator KiMoSys
      Created: 2018-07-28 16:46:57 UTC
      Updated: 2020-04-24 16:10:36 UTC
      Version: 2
      Status: (reviewed) 2018-07-28 16:47:40 UTC
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