Detail View - Data AccessID 111
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General Information
| Manuscript title | Time-dependent regulation of yeast glycolysis upon nitrogen starvation depends on cell history. |
| PubMed ID | 20232995 |
| Journal | IET Systems Biology |
| Year | 2010 |
| Authors | van Eunen K, Dool P, Canelas AB, Kiewiet J, Bouwman J, van Gulik WM, Westerhoff HV, Bakker BM |
| Affiliations | Vrije Universiteit Amsterdam, Department of Molecular Cell Physiology, Amsterdam, The Netherlands. |
| Keywords | yeast, glycolysis, glucose-limited chemostat, nitrogen starvation |
| Full text article |
vanEunen_2010.pdf
|
| Project name | not specified |
Experiment Description
| Organism | Saccharomyces cerevisiae |
| Strain | CEN.PK113-7D |
| Data type | metabolites at steady-state |
| Data units | mM |
| Execution date | not specified |
Experimental Details
| Temperature (°C) | 30 |
| pH | 5.0 ± 0.1 |
| Carbon source | glucose |
| Culture mode | chemostat |
| Process condition | aerobic |
| Dilution rate (h⁻¹) | 0.1 and 0.35 |
| Working volume (L) | 1.0 |
| Biomass concentration (g/L) | Yieldglu,X (g/g) = 0.45 ± 0.02 (D = 0.1h-1) and 0.29 ± 0.01 (D = 0.35h-1) |
| Medium composition | Defined mineral medium [1] in which glucose (42 mM) was the growth-limiting nutrient. |
| General protocol information |
Sampling method:
Samples were taken 15 min after the start of the fermentative capacity assay to the protocol described by Canelas et al. [2]. Quenching procedure: Quenching and washing of the sample was done with 100% and 80% (v/v) methanol/water, respectively, at -40ºC. Extraction technique: boiling ethanol Sample analyzing method: LC-ESI-MS |
| Methods description - Notes | Intracellular metabolite extraction was carried out using the boiling ethanol method [3], as described in Lange et al. [4]. U-13C-labelled cell extract was added to the pellets just before the extraction, as internal standard. Sample concentration was accomplished by evapora ... Read more |
| Data file |
 KIMODATAID111_v1.xlsx
|
| Alternative format(s) |
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Submission and curation
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Administrator KiMoSysFirst name: Administrator
Affiliation: INESC-ID/IST
Homepage: http://kdbio.inesc-id.pt/kimosys
Interests: mathematical modeling, accessible data, use of data
Created: 2018-07-30 14:53:24 UTC
Updated: 2020-04-24 16:10:36 UTC
Version: 1
Status: (reviewed) 2018-07-30 14:58:34 UTC
Views: 229
Downloads: 73
Associated Models
Here we can find relevant models associated with Data EntryID 111:
| Model EntryID |
Model name | Category | Model Type | Data used for | Access |
|---|---|---|---|---|---|
| 46 | vanEunen2012 - Yeast Glycolysis (glucose upshift) | Metabolism | ordinary differential equations | Model building and Model validation |  |
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