Repository » Data AccessID 122
Detail View - Data AccessID 122


General Information
Manuscript title Linking high-resolution metabolic flux phenotypes and transcriptional regulation in yeast modulated by the global regulator Gcn4p.
PubMed ID 19346491
Journal Proc Natl Acad Sci USA
Year 2009
Authors Joel F. Moxley, Michael C. Jewett, Maciek R. Antoniewicz, Silas G. Villas-Boas, Hal Alper, Robert T. Wheeler, Lily Tong, Alan G. Hinnebusch, Trey Ideker, Jens Nielsen, and Gregory Stephanopoulos
Affiliations Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139
Keywords amino acid stress response, fluxomics, gcn4, systems biology
Full text article Moxley_2009.pdf
Project name not specified

Experiment Description
Organism Saccharomyces cerevisiae
Strain WT S288C and gcn4-knockout
Data type flux measurements
Data units not specified
Execution date not specified

Experimental Details
Temperature (°C) 30
pH 5.0
Carbon source glucose
Culture mode chemostat
Process condition aerobic
Dilution rate (h⁻¹) 0.1
Working volume (L) 1.0
Biomass concentration (g/L) not specified
Medium composition

unsupplemented YNB minimal media.

General protocol information Flux analysis method: 13C constrained MFA

Platform: GC-MS

Methods description - Notes

Metabolic fluxes were determined using the Metran software, using the elementary metabolite units (EMU) algorithm [1, 2]. Metran accepts as input a user-defined metabolic network model consisting of biochemical reactions and atom transitions, and a set of 13C-measurements an ...

Read more
Data file
KIMODATAID122_v1.xlsx Preview file
Alternative format(s)
no file uploaded
Export metadata
RDF: metadataDataEntryID122.rdf
XML: metadataDataEntryID122.xml
Plain text: metadataDataEntryID122.txt
Share | Cite Data EntryID 122

Related Data: AccessID 61 | AccessID 62 | AccessID 69 | AccessID 70 | AccessID 93 | AccessID 97 | AccessID 98 | AccessID 99 | AccessID 100 | AccessID 111 | AccessID 115 | AccessID 117 | AccessID 120 | AccessID 121 | AccessID 123 | AccessID 124 | AccessID 128

Submission and curation

Entered by: Administrator KiMoSysFirst name: Administrator
Affiliation: INESC-ID/IST
Interests: mathematical modeling, accessible data, use of data

Created: 2018-09-12 09:46:26 UTC

Updated: 2020-04-24 16:10:37 UTC

Version: 1

Status: (reviewed) 2018-09-12 09:47:23 UTC

Views: 165

Downloads: 126

Associated Models

Here we can find relevant models associated with Data EntryID 122:

Model name Category Model Type Data used for Access Json

Associate models to data

- Several models can be associated.

New Model

Back | Top