Detail View - Data AccessID 99
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General Information
| Manuscript title | A model of yeast glycolysis based on a consistent kinetic characterisation of all its enzymes. |
| PubMed ID | 23831062 |
| Journal | FEBS Letters |
| Year | 2013 |
| Authors | Kieran Smallbone, Hanan L. Messiha, Kathleen M. Carroll, Catherine L. Winder, Naglis Malys, Warwick B. Dunn, Ettore Murabito, Neil Swainston, Joseph O. Dada, Farid Khan, Pınar Pir, Evangelos Simeonidis, Irena Spasić, Jill Wishart, Dieter Weichart, Neil W. Hayes, Daniel Jameson, David S. Broomhead, Stephen G. Oliver, Simon J. Gaskell, John E.G. McCarthy, Norman W. Paton, Hans V. Westerhoff, Douglas B. Kell, Pedro Mendes |
| Affiliations | Manchester Centre for Integrative Systems Biology, Manchester Institute of Biotechnology, The University of Manchester, UK |
| Keywords | Glycolysis, Systems biology, Enzyme kinetic, Isoenzyme, Modelling |
| Full text article |
Smallbone_2013.pdf
|
| Project name | — |
Experiment Description
| Organism | Saccharomyces cerevisiae |
| Strain | Y23925 |
| Data type | metabolites at steady-state |
| Data units | molecules/cell |
| Execution date | not specified |
Experimental Details
| Temperature (°C) | not specified |
| pH | not specified |
| Carbon source | glucose |
| Culture mode | chemostat |
| Process condition | aerobic |
| Dilution rate (h⁻¹) | µmax |
| Working volume (L) | not specified |
| Biomass concentration (g/L) | monitored by measuring the electrical capacitance of the culture. |
| Medium composition | not specified |
| General protocol information |
Sampling method:
10 ml of culture. Quenching procedure: 10 ml of culture solution into 40 ml of a 60:40 methanol/water solution [1] stored at a temperature of −47 °C [2]. Immediate separation of cells was performed by applying centrifugation (4000 g for 5 min) followed by removal of the quenching solution. Extraction technique: freezing-thawing in methanol, methanol-water Sample analyzing method: GC-MS, LC-MS |
| Methods description - Notes | Samples were analysed using two analytical platforms. Fructose‐1,6‐bisphosphate was quantified applying ultra-performance liquid chromatography mass spectrometry (UPLC‐MS) (Waters Acquity UPLC coupled to a ThermoFisher hybrid electrospray LTQ‐Orbitrap mass spectrometer (Ther ... Read more |
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Administrator KiMoSysFirst name: Administrator
Affiliation: INESC-ID/IST
Homepage: http://kdbio.inesc-id.pt/kimosys
Interests: mathematical modeling, accessible data, use of data
Created: 2018-07-16 15:49:58 UTC
Updated: 2020-04-24 16:10:35 UTC
Version: 2
Status: (reviewed) 2018-07-16 15:57:33 UTC
Views: 366
Downloads: 67
Associated Models
Here we can find relevant models associated with Data EntryID 99:
| Model EntryID |
Model name | Category | Model Type | Data used for | Access |
|---|---|---|---|---|---|
| 39 | smallbone18 | Metabolism | ordinary differential equations | Model validation |  |
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