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General Information info

Manuscript title Characterization of glucose transport mutants of Saccharomyces cerevisiae during a nutritional upshift reveals a correlation between metabolite levels and glycolytic flux.
PubMed ID 18042231
Journal FEMS Yeast Research
Year 2008
Authors Daniel Bosch, Mikael Johansson, Cecilia Ferndahl, Carl Johan Franzén, Christer Larsson and Lena Gustafsson
Affiliations Molecular Biotechnology and Chemical reaction Engineering, Department of Chemical and Biological Engineering, Chalmers University of Technology, Goeteborg, Sweden
Keywords Saccharomyces cerevisiae, extracellular, metabolites, glucose and fructose pulse
Full text article Downloadarticle Bosch_2008.pdf
Project name not specified

Experiment Description info

Organism Saccharomyces cerevisiae
Strain CEN.PK2-1C (W.T.), HXT1, HXT7 and TM6
Data type time-series data of metabolites
Data units (mM)
Execution date not specified

Experimental Details info

Temperature (0C) 30.0
pH 5.0
Carbon source ethanol,
Culture mode chemostat
Process condition aerobic
Dilution rate (h-1) 0.1
Working volume (L) 2.0
Biomass concentration (g/L) correlation of OD with biomass: 0.486 gDW/L OD
Medium composition

defined medium with 5 g/L glucose

General protocol information Sampling method: duplicate samples of 1ml each were steril filtered (0.22 µm), frozen in liquid N2 and stored at -20 ºC.

Quenching procedure:

Extraction technique: not used

Sample analyzing method: enzymatic

Methods description - Notes

Extracellular metabolites: glucose, ethanol and glycerol were performed using enzymatic combination kits (Boehringer-Mannheim GmbH, Mannheim, Germany).

Data file
Downloadmetabolites KIMODATAID69_v1.xlsx
Alternative format(s)
KIMODATAID69_wt_glucose_timeseries.csv
KIMODATAID69_wt_fructose_timeseries.csv
KIMODATAID69_TM6_glucose_timeseries.csv
KIMODATAID69_TM6_fructose_timeseries.csv
KIMODATAID69_HXT7_glucose_timeseries.csv
KIMODATAID69_HXT7_fructose_timeseries.csv
KIMODATAID69_HXT1_glucose_timeseries.csv
KIMODATAID69_HXT1_fructose_timeseries.csv
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Organism


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Submission and curation info

Entered by Administrator KiMoSysFirst name: Administrator
Affiliation: INESC-ID/IST
Homepage: http://kdbio.inesc-id.pt/kimosys
Interests: mathematical modeling, accessible data, use of data

Created 2013-06-21 09:14:42 UTC

Updated 2018-07-18 23:23:37 UTC

Version 1

Status (reviewed) 2013-12-06 17:17:38 UTC




Associated Models


Here we can find relevant models associated with Data EntryID 69:

Model
EntryID
Model name Category Model Type Data used for Access Json
28
Authors: Daniel Bosch, Mikael Johansson, Cecilia Ferndahl, Carl Johan Franzén, Christer Larsson and Lena Gustafsson

Original paper: Characterization of glucose transport mutants of Saccharomyces cerevisiae during a nutritional upshift reveals a correlation between metabolite levels and glycolytic flux.

Model for respiro-fermentative growth on glucose Metabolism ordinary differential equations Model building Visto4 {"affiliation":"Molecular Biotechnology and Chemical reaction Engineering, Department of Chemical and Biological Engineering, Chalmers University of Technology, Goeteborg, Sweden","article_file_name":null,"authors":"Daniel Bosch, Mikael Johansson, Cecilia Ferndahl, Carl Johan Franz\u00e9n, Christer Larsson and Lena Gustafsson","biomodels_id":"","category":"Metabolism","combine_archive_content_type":null,"combine_archive_file_name":null,"combine_archive_file_size":null,"combine_archive_updated_at":null,"comments":"","control":"2014-04-11T17:42:47Z","dilution_rate":"\u2014","id":28,"journal":"FEMS Yeast Research","keywords":"fitting, glucose pulse, Saccharomyce cerevisae, respiro-fermentative growth on glucose,","main_organism":"Saccharomyces cerevisiae","manuscript_title":"Characterization of glucose transport mutants of Saccharomyces cerevisiae during a nutritional upshift reveals a correlation between metabolite levels and glycolytic flux.","model_name":"Model for respiro-fermentative growth on glucose","model_type":"ordinary differential equations","organism_id":69,"project_name":"","pubmed_id":"18042231","review_journal_id":null,"sbml_file_name":"Bosch_2008.pdf","software":"http://www.matlab.com (MATLAB)","used_for":"---\n- Model building\n","year":2008} Administrator KiMoSys



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