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General Information
Manuscript title Oxygen dependence of metabolic fluxes and energy generation of Saccharomyces cerevisiae CEN.PK113-1A
PubMed ID 18613954
Journal BMC Systems Biology
Year 2008
Authors Paula Jouhten, Eija Rintala, Anne Huuskonen, Anu Tamminen, Mervi Toivari, Marilyn Wiebe, Laura Ruohonen, Merja Penttilä and Hannu Maaheimo
Affiliations VTT Technical Research Centre of Finland, Espoo, Finland
Keywords Pentose Phosphate Pathway, Flux Distribution, Flux Ratio, Metabolic Flux Analysis, Central Carbon Metabolism
Full text article Jouhten_2008.pdf
Project name not specified

Experiment Description
Organism Saccharomyces cerevisiae
Strain CEN.PK113-1A
Data type flux measurements
Data units normalised to the specific glucose uptake rate
Execution date not specified

Experimental Details
Temperature (°C) 30
pH 5.0
Carbon source glucose
Culture mode chemostat
Process condition aerobic and anaerobic
Dilution rate (h⁻¹) 0.1
Working volume (L) 2.5
Biomass concentration (g/L) see spreadsheet
Medium composition

Defined minimal medium [1], with 10 g glucose l-1 as carbon source, and supplemented with 10 mg ergosterol l-1 and 420 mg Tween 80 l-1 (a source of oleic acid).

General protocol information Flux analysis method: flux ratio, 13C constrained MFA

Platform: NMR

Methods description - Notes

The culture conditions and biomass determination and chemical and metabolite analyses are described in more detail by Wiebe et al. (2008) [2].
Biosyntheti ...

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Submission and curation

Entered by: Administrator KiMoSysFirst name: Administrator
Affiliation: INESC-ID/IST
Interests: mathematical modeling, accessible data, use of data

Created: 2018-08-23 21:59:13 UTC

Updated: 2020-04-24 16:10:37 UTC

Version: 1

Status: (reviewed) 2018-08-23 22:29:58 UTC

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Downloads: 28

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