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General Information
Manuscript title A comparative transcriptomic, fluxomic and metabolomic analysis of the response of Saccharomyces cerevisiae to increases in NADPH oxidation.
PubMed ID 22805527
Journal BMC Genomics
Year 2012
Authors Magalie Celton, Isabelle Sanchez, Anne Goelzer, Vincent Fromion, Carole Camarasa and Sylvie Dequin
Affiliations INRA, UMR1083 SPO, 2 place Viala, F-34060 Montpellier, France
Keywords NADPH, Pentose Phosphate Pathway, Acetoin, Intracellular Metabolite, NADPH Oxidation
Full text article Celton_2012.pdf
Project name not specified


Experiment Description
Organism Saccharomyces cerevisiae
Strain 59A NADPH-Bdh
Data type flux measurements
Data units mmol/100 mmol glucose
Execution date not specified


Experimental Details
Temperature (°C) 28
pH 5.0
Carbon source glucose,
Culture mode batch
Process condition anaerobic
Dilution rate (h⁻¹)
Working volume (L) 0.01
Biomass concentration (g/L) for acetoin 0 mM = 2.9g, for acetoin 100 mM = 2.55g, for acetoin 200 mM = 2.65g and for acetoin 300 mM = 2.17g
Medium composition

2X SD minimal medium (13.4% yeast nitrogen base without amino acids, 100% glucose). The SD minimal medium was supplemented with 1.25 mg/l ergosterol, 0.164 g/l Tween 80, and 0.35 mg/l oleic acid, to satisfy the lipid requirements of the yeast cells during anaerobic growth. Acetoin was added at various concentrations (from 0 to 300 mM) at the start of fermentation, as previously described by Celton et al. [1].

General protocol information Flux analysis method: 13C constrained MFA

Platform: GC-MS

Methods description - Notes

At an OD600 of 3.0, corresponding to mid-exponential growth phase, the concentration of extracellular metabolites (glucose, glycerol, organic acids) was measured in the supernatant (these data will be used to constrain the stoichiometric model, as described below). Cells wer ...

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Submission and curation

Entered by: Administrator KiMoSysFirst name: Administrator
Affiliation: INESC-ID/IST
Homepage: http://kdbio.inesc-id.pt/kimosys
Interests: mathematical modeling, accessible data, use of data

Created: 2018-08-19 19:03:08 UTC

Updated: 2020-04-24 16:10:36 UTC

Version: 0

Status: (reviewed) 2018-08-19 19:03:43 UTC

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Downloads: 0




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