Detail View - Data AccessID 70
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General Information
| Manuscript title | Characterization of glucose transport mutants of Saccharomyces cerevisiae during a nutritional upshift reveals a correlation between metabolite levels and glycolytic flux. |
| PubMed ID | 18042231 |
| Journal | FEMS Yeast Research |
| Year | 2008 |
| Authors | Daniel Bosch, Mikael Johansson, Cecilia Ferndahl, Carl Johan Franzén, Christer Larsson and Lena Gustafsson |
| Affiliations | Molecular Biotechnology and Chemical reaction Engineering, Department of Chemical and Biological Engineering, Chalmers University of Technology, Goeteborg, Sweden |
| Keywords | Saccharomyces cerevisiae, intracellular, metabolites, glucose and fructose pulse |
| Full text article |
Bosch_2008.pdf
|
| Project name | not specified |
Experiment Description
| Organism | Saccharomyces cerevisiae |
| Strain | CEN.PK2-1C (W.T.), HXT1, HXT7 and TM6 |
| Data type | time-series data of metabolites |
| Data units | (mM) |
| Execution date | not specified |
Experimental Details
| Temperature (°C) | 30.0 |
| pH | 5.0 |
| Carbon source | ethanol |
| Culture mode | chemostat |
| Process condition | aerobic |
| Dilution rate (h⁻¹) | 0.1 |
| Working volume (L) | 2.0 |
| Biomass concentration (g/L) | correlation of OD with biomass: 0.486 gDW/L OD |
| Medium composition | defined medium with 5 g/L glucose |
| General protocol information |
Sampling method:
for all metabolites, except ATP, six samples of 3 mL each Quenching procedure: samples were directly transferred into tubes with 10mL of methanol maintained at -48ºC; the final concentration of methanol (v/v) was about 75%. Extraction technique: methanol Sample analyzing method: enzymatic |
| Methods description - Notes | Intracellular metabolites - For all metabolites, except ATP, six samples of 3 mL each were directly transferred into preweighed tubes with 10 mL of methanol maintained at -48 ºC; the final concentration of methanol (v/v) was about 75%. The extraction was performed as describ ... ----------------References-----------------
|
| Data file |
 KIMODATAID70_v1.xlsx
|
| Alternative format(s) |
KIMODATAID70_wt_glcucose_timeseries.csv
Preview
KIMODATAID70_wt_fructose_timeseries.csv Preview KIMODATAID70_TM6_glucose_timeseries.csv Preview KIMODATAID70_TM6_fructose_timeseries.csv Preview KIMODATAID70_HXT7_glucose_timeseries.csv Preview KIMODATAID70_HXT7_fructose_timeseries.csv Preview KIMODATAID70_HXT1_glucose_timeseries.csv Preview KIMODATAID70_HXT1_fructose_timeseries.csv Preview |
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RDF:
metadataDataEntryID70.rdf
XML: metadataDataEntryID70.xml Plain text: metadataDataEntryID70.txt |
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Submission and curation
Entered by:
Administrator KiMoSysFirst name: Administrator
Affiliation: INESC-ID/IST
Homepage: http://kdbio.inesc-id.pt/kimosys
Interests: mathematical modeling, accessible data, use of data
Created: 2013-06-21 14:12:31 UTC
Updated: 2020-04-24 16:10:33 UTC
Version: 1
Status: (reviewed) 2013-12-06 17:17:38 UTC
Views: 319
Downloads: 63
Associated Models
Here we can find relevant models associated with Data EntryID 70:
| Model EntryID |
Model name | Category | Model Type | Data used for | Access |
|---|---|---|---|---|---|
| 28 | Model for respiro-fermentative growth on glucose | Metabolism | ordinary differential equations | Model building |  |
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