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General Information info

Manuscript title Characterization of glucose transport mutants of Saccharomyces cerevisiae during a nutritional upshift reveals a correlation between metabolite levels and glycolytic flux.
PubMed ID 18042231
Journal FEMS Yeast Research
Year 2008
Authors Daniel Bosch, Mikael Johansson, Cecilia Ferndahl, Carl Johan Franzén, Christer Larsson and Lena Gustafsson
Affiliations Molecular Biotechnology and Chemical reaction Engineering, Department of Chemical and Biological Engineering, Chalmers University of Technology, Goeteborg, Sweden
Keywords Saccharomyces cerevisiae, intracellular, metabolites, glucose and fructose pulse
Full text article Downloadarticle Bosch_2008.pdf
Project name not specified

Experiment Description info

Organism Saccharomyces cerevisiae
Strain CEN.PK2-1C (W.T.), HXT1, HXT7 and TM6
Data type time-series data of metabolites
Data units (mM)
Execution date not specified

Experimental Details info

Temperature (0C) 30.0
pH 5.0
Carbon source ethanol,
Culture mode chemostat
Process condition aerobic
Dilution rate (h-1) 0.1
Working volume (L) 2.0
Biomass concentration (g/L) correlation of OD with biomass: 0.486 gDW/L OD
Medium composition

defined medium with 5 g/L glucose

General protocol information Sampling method: for all metabolites, except ATP, six samples of 3 mL each

Quenching procedure: samples were directly transferred into tubes with 10mL of methanol maintained at -48ºC; the final concentration of methanol (v/v) was about 75%.

Extraction technique: methanol

Sample analyzing method: enzymatic

Methods description - Notes

Intracellular metabolites - For all metabolites, except ATP, six samples of 3 mL each were directly transferred into preweighed tubes with 10 mL of methanol maintained at -48 ºC; the final concentration of methanol (v/v) was about 75%. The extraction was performed as described previously [1]. The extraction and quantification of ATP was performed separately. Triplicate samples of 1 mL each were added to 1.2 mL of 0.51 M TCA, thereby extracting ATP as described earlier in Gustafsson [1]. The ATP concentration was analysed in triplicate on a Packard Pico-Lite luminometer (Packard Instruments, Downers Grove, IL) using ATP bioluminescence assay kits (CLSII) (Roche Diagnostics, Mannheim, Germany).

----------------References-----------------
[1] Gustafsson L (1979). The ATP pool in relation to the production of glycerol and heat during growth of the halotolerant yeast Debaryomyces hansenii. Arch Microbiol 120: 15–23. http://doi.org/cdbkd6

Data file
Downloadmetabolites KIMODATAID70_v1.xlsx
Alternative format(s)
KIMODATAID70_wt_glcucose_timeseries.csv
KIMODATAID70_wt_fructose_timeseries.csv
KIMODATAID70_TM6_glucose_timeseries.csv
KIMODATAID70_TM6_fructose_timeseries.csv
KIMODATAID70_HXT7_glucose_timeseries.csv
KIMODATAID70_HXT7_fructose_timeseries.csv
KIMODATAID70_HXT1_glucose_timeseries.csv
KIMODATAID70_HXT1_fructose_timeseries.csv
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Organism


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Submission and curation info

Entered by Administrator KiMoSysFirst name: Administrator
Affiliation: INESC-ID/IST
Homepage: http://kdbio.inesc-id.pt/kimosys
Interests: mathematical modeling, accessible data, use of data

Created 2013-06-21 14:12:31 UTC

Updated 2018-07-18 23:18:02 UTC

Version 1

Status (reviewed) 2013-12-06 17:17:38 UTC




Associated Models


Here we can find relevant models associated with Data EntryID 70:

Model
EntryID
Model name Category Model Type Data used for Access Json
28
Authors: Daniel Bosch, Mikael Johansson, Cecilia Ferndahl, Carl Johan Franzén, Christer Larsson and Lena Gustafsson

Original paper: Characterization of glucose transport mutants of Saccharomyces cerevisiae during a nutritional upshift reveals a correlation between metabolite levels and glycolytic flux.

Model for respiro-fermentative growth on glucose Metabolism ordinary differential equations Model building Visto4 {"affiliation":"Molecular Biotechnology and Chemical reaction Engineering, Department of Chemical and Biological Engineering, Chalmers University of Technology, Goeteborg, Sweden","article_file_name":null,"authors":"Daniel Bosch, Mikael Johansson, Cecilia Ferndahl, Carl Johan Franz\u00e9n, Christer Larsson and Lena Gustafsson","biomodels_id":"","category":"Metabolism","combine_archive_content_type":null,"combine_archive_file_name":null,"combine_archive_file_size":null,"combine_archive_updated_at":null,"comments":"","control":"2014-04-11T17:42:47Z","dilution_rate":"\u2014","id":28,"journal":"FEMS Yeast Research","keywords":"fitting, glucose pulse, Saccharomyce cerevisae, respiro-fermentative growth on glucose,","main_organism":"Saccharomyces cerevisiae","manuscript_title":"Characterization of glucose transport mutants of Saccharomyces cerevisiae during a nutritional upshift reveals a correlation between metabolite levels and glycolytic flux.","model_name":"Model for respiro-fermentative growth on glucose","model_type":"ordinary differential equations","organism_id":69,"project_name":"","pubmed_id":"18042231","review_journal_id":null,"sbml_file_name":"Bosch_2008.pdf","software":"http://www.matlab.com (MATLAB)","used_for":"---\n- Model building\n","year":2008} Administrator KiMoSys



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