DataEntryID 70
    General information
      Manuscript title: Characterization of glucose transport mutants of Saccharomyces cerevisiae during a nutritional upshift reveals a correlation between metabolite levels and glycolytic flux.
      PubMed ID: http://www.ncbi.nlm.nih.gov/pubmed/18042231
      Journal: FEMS Yeast Research
      Year: 2008
      Authors: Daniel Bosch, Mikael Johansson, Cecilia Ferndahl, Carl Johan Franzén, Christer Larsson and Lena Gustafsson
      Affiliations: Molecular Biotechnology and Chemical reaction Engineering, Department of Chemical and Biological Engineering, Chalmers University of Technology, Goeteborg, Sweden
      Keywords: Saccharomyces cerevisiae, intracellular, metabolites, glucose and fructose pulse
      Full text article: https://kimosys.org/rails/active_storage/blobs/eyJfcmFpbHMiOnsibWVzc2FnZSI6IkJBaHBBbzBFIiwiZXhwIjpudWxsLCJwdXIiOiJibG9iX2lkIn19--b54fdcca9f31637e1a24ea8f68c2254d0d3ecb4d/Bosch_2008.pdf
      Project name: not specified

    Experiment description
      Organism: Saccharomyces cerevisiae
      Strain: CEN.PK2-1C (W.T.), HXT1, HXT7 and TM6
      Data type: time-series data of metabolites
      Data units: (mM)
      Execution date: not specified

    Experimental details
      Temperature (°C): 30.0
      pH: 5.0
      Carbon source: ethanol
      Culture mode: chemostat
      Process condition: aerobic
      Dilution rate (h⁻¹): 0.1
      Working volume: 2.0 L
      Biomass concentration (g/L): correlation of OD with biomass: 0.486 gDW/L OD
      Medium composition: defined medium with 5 g/L glucose
      General protocol information: Sampling Method: for all metabolites, except ATP, six samples of 3 mL each; Quenching: samples were directly transferred into tubes with 10mL of methanol maintained at -48ºC; the final concentration of methanol (v/v) was about 75%.; Extraction list: methanol; Analysis list: enzymatic;
      Methods description: Intracellular metabolites - For all metabolites, except ATP, six samples of 3 mL each were directly transferred into preweighed tubes with 10 mL of methanol maintained at -48 ºC; the final concentration of methanol (v/v) was about 75%. The extraction was performed as described previously [1]. The extraction and quantification of ATP was performed separately. Triplicate samples of 1 mL each were added to 1.2 mL of 0.51 M TCA, thereby extracting ATP as described earlier in Gustafsson [1]. The ATP concentration was analysed in triplicate on a Packard Pico-Lite luminometer (Packard Instruments, Downers Grove, IL) using ATP bioluminescence assay kits (CLSII) (Roche Diagnostics, Mannheim, Germany).

----------------References-----------------
[1] Gustafsson L (1979). The ATP pool in relation to the production of glycerol and heat during growth of the halotolerant yeast Debaryomyces hansenii. Arch Microbiol 120: 15–23. http://doi.org/cdbkd6 
      Data file: http://kimosys.org/repository/70/download?parameter=1164; 
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    Submission and curation
      Entered by: Administrator KiMoSys
      Created: 2013-06-21 14:12:31 UTC
      Updated: 2020-04-24 16:10:33 UTC
      Version: 1
      Status: (reviewed) 2013-12-06 17:17:38 UTC
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