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General Information
Manuscript title Metabolome, transcriptome and metabolic flux analysis of arabinose fermentation by engineered Saccharomyces cerevisiae.
PubMed ID 20816840
Journal Metabolic Engineering
Year 2010
Authors H. Wouter Wisselink, Chiara Cipollina, Bart Oud, BarbaraCrimi, Joseph J. Heijnen, Jack T. Pronk, Antonius J.A.van Maris
Affiliations Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands.
Keywords Saccharomyces cerevisiae, Evolutionary engineering, Arabinose, Transcriptomics, Metabolomics, Metabolic flux analysis
Full text article Wisselink_2010.pdf
Project name not specified

Experiment Description
Organism Saccharomyces cerevisiae
Strain IMS0001 and IMS0002
Data type flux measurements
Data units normalised to the specific glucose uptake rate
Execution date not specified

Experimental Details
Temperature (°C) 30
pH 5.0
Carbon source glucose and arabinose
Culture mode chemostat
Process condition anaerobic
Dilution rate (h⁻¹) 0.03
Working volume (L) 1.0
Biomass concentration (g/L) Biomass yield (g g−1) = 0.066±0.001 (IMS0001, Glucose), 0.072±0.003 (IMS0002, Glucose) and 0.075±0.001 (IMS0002, Arabinose)
Medium composition

Cultures were performed in MY supplemented with 0.01 g l−1 ergosterol and 0.42 g l−1 Tween 80 dissolved in ethanol, silicon antifoam, vitamin solution and trace elements [7], and 20 g l−1 glucose (MYG) or arabinose (MYA).

General protocol information Flux analysis method: 13C constrained MFA

Platform: LC-MS

Methods description - Notes

Intracellular metabolic fluxes were calculated through metabolic flux balancing using a compartmented stoichiometric model that was previously developed for describing aerobic growth on glucose [1,2] and modified to describe anaerobic growth on glucose and arabinose. Reactio ...

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Submission and curation

Entered by: Administrator KiMoSysFirst name: Administrator
Affiliation: INESC-ID/IST
Interests: mathematical modeling, accessible data, use of data

Created: 2018-08-28 16:39:16 UTC

Updated: 2020-04-24 16:10:37 UTC

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Status: (unreviewed)

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