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General Information
Manuscript title Transcriptional regulation of respiration in yeast metabolizing differently repressive carbon substrates
PubMed ID 20167065
Journal BMC Systems Biology
Year 2010
Authors Sarah-Maria Fendt, Uwe Sauer
Affiliations Institute of Molecular Systems Biology, ETH Zurich, Zurich, Switzerland
Keywords Saccharomyces cerevisiae, Flux Analysis, FY4, Yeast
Full text article Fluxes_Yeast_Fendt_2010.pdf
Project name not specified

Experiment Description
Organism Saccharomyces cerevisiae
Strain FY4
Data type flux measurements
Data units mmol/g*h
Execution date 2009-03-09

Experimental Details
Temperature (°C) 30
pH not applicable
Carbon source glucose, galactose, mannose, pyruvate
Culture mode batch
Process condition aerobic
Dilution rate (h⁻¹) not applicable
Working volume (L) 0,0012
Biomass concentration (g/L) see Table 1
Medium composition

minimal medium with either glucose, mannose, galactose or pyruvate as sole carbon source

General protocol information Flux analysis method: 13C constrained MFA

Platform: GC-MS

Methods description - Notes

Strains, medium and cultivation conditions - Allliquid cultivations were carried out using minimal medium as described in Blank and Sauer (2004) [1]. The pre-cultures were always cultured in glucose minimal medium. Other carbon sources or labeled substrates were only added t ...

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Submission and curation

Entered by: Hugo MochãoFirst name: Hugo
Affiliation: FCT-UNL
Interests: Proteomics

Created: 2020-11-23 18:16:21 UTC

Updated: 2020-11-24 14:22:56 UTC

Version: 2

Status: (unreviewed)

Views: 184

Downloads: 37

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