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General Information
Manuscript title Temporal system-level organization of the switch from glycolytic to gluconeogenic operation in yeast.
PubMed ID 23549479
Journal Molecular Systems Biology
Year 2013
Authors Guillermo G Zampar, Anne Kümmel, Jennifer Ewald, Stefan Jol, Bastian Niebel, Paola Picotti, Ruedi Aebersold, Uwe Sauer, Nicola Zamboni and Matthias Heinemann
Affiliations ETH Zurich, Institute of Molecular Systems Biology, Zurich, Switzerland
Keywords diauxic shift, metabolome, S. cerevisiae, extracelullar
Full text article Zampar_2013.pdf
Project name not specified

Experiment Description
Organism Saccharomyces cerevisiae
Strain FY4
Data type time-series data of metabolites
Data units (mM) and (gDW/L)
Execution date not specified

Experimental Details
Temperature (°C) 30.0
pH 5.0
Carbon source glucose
Culture mode batch
Process condition aerobic
Dilution rate (h⁻¹)
Working volume (L) 2.0
Biomass concentration (g/L) correlation of OD with biomass: 0.486 gDW/L OD
Medium composition

minimal defined medium with 5 g/L glucose

General protocol information Sampling method: 1 ml samples and centrifuged for 4 min at 4000, r.p.m at 4 ºC

Quenching procedure:

Extraction technique: not used

Sample analyzing method: HPLC-UV/RI

Methods description - Notes

Biomass concentrations were monitored by measuring optical density (OD) at 600nm with a spectrophotometer (model Novaspec II, Pharmacia Biotech, USA). The correlation of OD with biomass dry weight (0.486 gDW/l OD) had previously been determined from a batch culture grown on ...

[1] Heer D, Sauer U (2008). Identification of furfural as a key toxin in lignocellulosic hydrolysates and evolution of a tolerant yeast strain. Microb Biotechnol 1: 497–506.

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Submission and curation

Entered by: Administrator KiMoSysFirst name: Administrator
Affiliation: INESC-ID/IST
Interests: mathematical modeling, accessible data, use of data

Created: 2013-06-11 18:31:52 UTC

Updated: 2020-04-24 16:10:32 UTC

Version: 0

Status: (reviewed) 2013-12-06 17:17:38 UTC

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Downloads: 37

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