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General Information
Manuscript title Global analysis of protein expression in yeast.
PubMed ID 14562106
Journal Nature
Year 2003
Authors Sina Ghaemmaghami, Won-Ki Huh, Kiowa Bower, Russell W. Howson, Archana Belle, Noah Dephoure, Erin K. O'Shea & Jonathan S. Weissman
Affiliations Howard Hughes Medical Institute, University of California–San Francisco, San Francisco, California 94143-2240, USA
Keywords yeast, protein expression, S. cerevisiae
Full text article Ghaemmaghami_2003.pdf
Project name not specified


Experiment Description
Organism Saccharomyces cerevisiae
Strain TAP-tagged strain
Data type enzyme/protein concentrations
Data units molecules/cell
Execution date not specified


Experimental Details
Temperature (°C) 30
pH not specified
Carbon source glucose,
Culture mode chemostat
Process condition aerobic
Dilution rate (h⁻¹)
Working volume (L) 0.0017
Biomass concentration (g/L) OD of ~0.7
Medium composition

YEPD media.

General protocol information Measurement method: LC-MS/MS

Methods description - Notes

Extract preparation and quantification of protein levels: Cultures (1.7 ml) of tagged strains were grown in 96-well format to log phase, and total cell
extracts were examined by SDS–polyacrylamide gel electrophoresis (PAGE)/western blot analysis. Before the quantitativ ...

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Submission and curation

Entered by: Administrator KiMoSysFirst name: Administrator
Affiliation: INESC-ID/IST
Homepage: http://kdbio.inesc-id.pt/kimosys
Interests: mathematical modeling, accessible data, use of data

Created: 2018-07-16 15:29:11 UTC

Updated: 2020-04-24 16:10:35 UTC

Version: 0

Status: (reviewed) 2018-07-16 15:29:45 UTC

Views: 6

Downloads: 0




Associated Models

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EntryID
Model name Category Model Type Data used for Access Json
39 smallbone18 Metabolism ordinary differential equations Model validation {"id":39,"organism_id":null,"comments":"Original model source: in JWS online database.","sbml_file_name":"Smallbone_2013.pdf","article_file_name":null,"category":"Metabolism","used_for":"---\n- Model validation\n","model_type":"ordinary differential equations","pubmed_id":"23831062","dilution_rate":"","name_of_model":"smallbone18","manuscript_title":"A model of yeast glycolysis based on a consistent kinetic characterisation of all its enzymes.","authors":"Kieran Smallbone, Hanan L. Messiha, Kathleen M. Carroll, Catherine L. Winder, Naglis Malys, Warwick B. Dunn, Ettore Murabito, Neil Swainston, Joseph O. Dada, Farid Khan, Pınar Pir, Evangelos Simeonidis, Irena Spasić, Jill Wishart, Dieter Weichart, Neil W.","journal":"FEBS Letters","affiliation":"Manchester Centre for Integrative Systems Biology, Manchester Institute of Biotechnology, The University of Manchester, UK","project_name":"","biomodels_id":"smallbone18","keywords":"Glycolysis, Systems biology, Enzyme kinetic, Isoenzyme, Modelling","software":"Copasi (www.copasi.org)","control":null,"main_organism":"Saccharomyces cerevisiae","year":2013,"combine_archive_file_name":null,"combine_archive_content_type":null,"combine_archive_file_size":null,"combine_archive_updated_at":null,"review_journal_id":null,"doi":null} Administrator KiMoSys



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