DataEntryID 69 General information Manuscript title: Characterization of glucose transport mutants of Saccharomyces cerevisiae during a nutritional upshift reveals a correlation between metabolite levels and glycolytic flux. PubMed ID: http://www.ncbi.nlm.nih.gov/pubmed/18042231 Journal: FEMS Yeast Research Year: 2008 Authors: Daniel Bosch, Mikael Johansson, Cecilia Ferndahl, Carl Johan Franzén, Christer Larsson and Lena Gustafsson Affiliations: Molecular Biotechnology and Chemical reaction Engineering, Department of Chemical and Biological Engineering, Chalmers University of Technology, Goeteborg, Sweden Keywords: Saccharomyces cerevisiae, extracellular, metabolites, glucose and fructose pulse Full text article: https://kimosys.org/rails/active_storage/blobs/eyJfcmFpbHMiOnsibWVzc2FnZSI6IkJBaHBBb3NFIiwiZXhwIjpudWxsLCJwdXIiOiJibG9iX2lkIn19--97c64455c8fc6690ad0c169d7fd1238f594c81cc/Bosch_2008.pdf Project name: not specified Experiment description Organism: Saccharomyces cerevisiae Strain: CEN.PK2-1C (W.T.), HXT1, HXT7 and TM6 Data type: time-series data of metabolites Data units: (mM) Execution date: not specified Experimental details Temperature (°C): 30.0 pH: 5.0 Carbon source: ethanol Culture mode: chemostat Process condition: aerobic Dilution rate (h⁻¹): 0.1 Working volume: 2.0 L Biomass concentration (g/L): correlation of OD with biomass: 0.486 gDW/L OD Medium composition: defined medium with 5 g/L glucose General protocol information: Sampling Method: duplicate samples of 1ml each were steril filtered (0.22 µm), frozen in liquid N2 and stored at -20 ºC.; Quenching: —; Extraction list: not used; Analysis list: enzymatic; Methods description: Extracellular metabolites: glucose, ethanol and glycerol were performed using enzymatic combination kits (Boehringer-Mannheim GmbH, Mannheim, Germany). Data file: http://kimosys.org/repository/69/download?parameter=1162; Alternative formats: http://kimosys.org/repository/69/attached_files/87/download; http://kimosys.org/repository/69/attached_files/86/download; http://kimosys.org/repository/69/attached_files/85/download; http://kimosys.org/repository/69/attached_files/84/download; http://kimosys.org/repository/69/attached_files/83/download; http://kimosys.org/repository/69/attached_files/82/download; http://kimosys.org/repository/69/attached_files/81/download; http://kimosys.org/repository/69/attached_files/80/download; Submission and curation Entered by: Administrator KiMoSys Created: 2013-06-21 09:14:42 UTC Updated: 2020-04-24 16:10:33 UTC Version: 1 Status: (reviewed) 2013-12-06 17:17:38 UTC Views: 309 Downloads: 58