DataEntryID 122
    General information
      Manuscript title: Linking high-resolution metabolic flux phenotypes and transcriptional regulation in yeast modulated by the global regulator Gcn4p.
      PubMed ID: http://www.ncbi.nlm.nih.gov/pubmed/19346491
      Journal: Proc Natl Acad Sci USA
      Year: 2009
      Authors: Joel F. Moxley, Michael C. Jewett, Maciek R. Antoniewicz, Silas G. Villas-Boas, Hal Alper, Robert T. Wheeler, Lily Tong, Alan G. Hinnebusch, Trey Ideker, Jens Nielsen, and Gregory Stephanopoulos
      Affiliations: Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139
      Keywords: amino acid stress response, fluxomics, gcn4, systems biology
      Full text article: https://kimosys.org/rails/active_storage/blobs/eyJfcmFpbHMiOnsibWVzc2FnZSI6IkJBaHBBdWtFIiwiZXhwIjpudWxsLCJwdXIiOiJibG9iX2lkIn19--bdb62272da5392f1b6c6341b6cc16d071dabca44/Moxley_2009.pdf
      Project name: not specified

    Experiment description
      Organism: Saccharomyces cerevisiae
      Strain: WT S288C and gcn4-knockout
      Data type: flux measurements
      Data units: not specified
      Execution date: not specified

    Experimental details
      Temperature (°C): 30
      pH: 5.0
      Carbon source: glucose
      Culture mode: chemostat
      Process condition: aerobic
      Dilution rate (h⁻¹): 0.1
      Working volume: 1.0 L
      Biomass concentration (g/L): not specified
      Medium composition: unsupplemented YNB minimal media.
      General protocol information: Type analysis list: 13C constrained MFA; Platform list: GC-MS;
      Methods description: Metabolic fluxes were determined using the Metran software, using the elementary metabolite units (EMU) algorithm [1, 2]. Metran accepts as input a user-defined metabolic network model consisting of biochemical reactions and atom transitions, and a set of 13C-measurements and external fluxes; it produces as output metabolic fluxes for the entire network, confidence intervals for all fluxes, and statistical analysis of the goodness-of-fit [3]. 
--------------------------------------------References--------------------------------------- 
[1] Yoo H, Antoniewicz MR, Stephanopoulos G, Kelleher JK(2008). J Biol Chem 283:20621–20627. http://doi.org/fsrtj5
[2] Antoniewicz MR, Kelleher JK, Stephanopoulos G (2007). Metab Eng 9:68–86. http://doi.org/b97p7s
[3] Antoniewicz MR, Kelleher JK, Stephanopoulos G (2006). Metab Eng, 324–337. http://doi.org/fbgz85
      Data file: http://kimosys.org/repository/122/download?parameter=1256; 
      Alternative formats: no files uploaded

    Submission and curation
      Entered by: Administrator KiMoSys
      Created: 2018-09-12 09:46:26 UTC
      Updated: 2020-04-24 16:10:37 UTC
      Version: 1
      Status: (reviewed) 2018-09-12 09:47:23 UTC
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      Downloads: 155