DataEntryID 111 General information Manuscript title: Time-dependent regulation of yeast glycolysis upon nitrogen starvation depends on cell history. PubMed ID: http://www.ncbi.nlm.nih.gov/pubmed/20232995 Journal: IET Systems Biology Year: 2010 Authors: van Eunen K, Dool P, Canelas AB, Kiewiet J, Bouwman J, van Gulik WM, Westerhoff HV, Bakker BM Affiliations: Vrije Universiteit Amsterdam, Department of Molecular Cell Physiology, Amsterdam, The Netherlands. Keywords: yeast, glycolysis, glucose-limited chemostat, nitrogen starvation Full text article: https://kimosys.org/rails/active_storage/blobs/eyJfcmFpbHMiOnsibWVzc2FnZSI6IkJBaHBBdE1FIiwiZXhwIjpudWxsLCJwdXIiOiJibG9iX2lkIn19--05626a99f9b06c4519a9b4684bf8ed27ec557055/vanEunen_2010.pdf Project name: not specified Experiment description Organism: Saccharomyces cerevisiae Strain: CEN.PK113-7D Data type: metabolites at steady-state Data units: mM Execution date: not specified Experimental details Temperature (°C): 30 pH: 5.0 ± 0.1 Carbon source: glucose Culture mode: chemostat Process condition: aerobic Dilution rate (h⁻¹): 0.1 and 0.35 Working volume: 1.0 L Biomass concentration (g/L): Yieldglu,X (g/g) = 0.45 ± 0.02 (D = 0.1h-1) and 0.29 ± 0.01 (D = 0.35h-1) Medium composition: Defined mineral medium [1] in which glucose (42 mM) was the growth-limiting nutrient. General protocol information: Sampling Method: Samples were taken 15 min after the start of the fermentative capacity assay to the protocol described by Canelas et al. [2].; Quenching: Quenching and washing of the sample was done with 100% and 80% (v/v) methanol/water, respectively, at -40ºC.; Extraction list: boiling ethanol; Analysis list: LC-ESI-MS; Methods description: Intracellular metabolite extraction was carried out using the boiling ethanol method [3], as described in Lange et al. [4]. U-13C-labelled cell extract was added to the pellets just before the extraction, as internal standard. Sample concentration was accomplished by evaporation under vacuum, as described by Mashego et al. [5]. The concentrations of the intracellular metabolites were determined by electrospray-ionisation liquid-chromatography mass spectrometry/mass spectrometry (ESI-LC-MS/MS) [6] and the quantification was based on isotope dilution mass spectrometry (IDMS) [5, 7]. Adenosine triphosphate (ATP), adenosine diphosphate (ADP) and adenosine monophosphate (AMP) were analysed by ion-pairing reversed-phase ESI-LC-MS/MS as described in [8]. ---------------------------------------References-------------------------------------- [1] VERDUYN C., POSTMA E., SCHEFFERS W.A., VAN DIJKEN J.P.: Yeast, 1992, 8, (7), pp. 501–517. http://doi.org/fgpqk3 [2] CANELAS A.B., RAS C., TEN PIERICK A., VAN DAM J.C., HEIJNEN J.J., VAN GULIK W.M., Metabolomics, 2008, 4, (3), pp. 226–239. http://doi.org/dtqddf [3] GONZALEZ B., FRANCOIS J., RENAUD M., Yeast, 1997, 13, (14), pp. 1347–1355. http://doi.org/cqztns [4] LANGE H.C., EMAN M., VAN ZUIJLEN G., ET AL., Biotechnol. Bioeng., 2001, 75, (4), pp. 406–415. http://doi.org/d6wqfv [5] MASHEGO M.R., WU L., VAN DAM J.C., ET AL., Biotechnol. Bioeng., 2004, 85, (6), pp. 620–628. http://doi.org/chdxwz [6] VAN DAM J.C., EMAN M.R., FRANK J., LANGE H.C., VAN DEDEM G.W.K., HEIJNEN S.J., Anal. Chim. Acta, 2002, 460, (2), pp. 209–218. http://doi.org/ck9bk6 [7] WU L., MASHEGO M.R., VAN DAM J.C., ET AL., Anal. Biochem., 2005, 336, (2), pp. 164–171. http://doi.org/dp4h5k [8] SEIFAR R.M., RAS C., VAN DAM J.C., VAN GULIK W.M., HEIJNEN J.J., VAN WINDEN W.A., Anal. Biochem., 2009, 388, (2), pp. 213–219. http://doi.org/cpxjw9 Data file: http://kimosys.org/repository/111/download?parameter=1234; Alternative formats: no files uploaded Submission and curation Entered by: Administrator KiMoSys Created: 2018-07-30 14:53:24 UTC Updated: 2020-04-24 16:10:36 UTC Version: 1 Status: (reviewed) 2018-07-30 14:58:34 UTC Views: 169 Downloads: 55