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General Information info

Manuscript title A multi-level study of recombinant Pichia pastoris in different oxygen conditions.
PubMed ID 20969759
Journal BMC Systems Biology
Year 2010
Authors Kristin Baumann, Marc Carnicer, Martin Dragosits, Alexandra B Graf, Johannes Stadlmann, Paula Jouhten, Hannu Maaheimo, Brigitte Gasser, Joan Albiol, Diethard Mattanovich, Pau Ferrer
Affiliations Department of Chemical Engineering, Autonomous University of Barcelona, Spain
Keywords Hypoxic Condition, Unfold Protein Response, Pentose Phosphate Pathway, Metabolic Flux, Flux Ratio
Full text article Downloadarticle Baumann_2010.pdf
Project name not specified

Experiment Description info

Organism Pichia pastoris
Strain X-33/pGAPaA_Fab and X-33/pGAPaA
Data type flux measurements
Data units (mmol/g.h)
Execution date not specified

Experimental Details info

Temperature (0C) 25
pH 5
Carbon source glucose,
Culture mode chemostat
Process condition aerobic
Dilution rate (h-1) 0.1
Working volume (L) 1.0
Biomass concentration (g/L) for O2 21% = 0.47 ± 0.02, for O2 11% = 0.45 ± 0.01 and for O2 8% = 0.25 ± 0.01
Medium composition

Minimal medium

General protocol information Flux analysis method: flux ratio, 13C constrained MFA

Platform: NMR

Methods description - Notes

Intracellular metabolic fluxes were determined using 13C-NMR derived flux ratios as additional experimental constraints to solve the MFA system [1]. The complete model for the calculation of intracellular fluxes, comprised 33 (normoxic condition) and 34 (oxygen-limiting and hypoxic conditions) metabolic reactions. The measured uptake and excretion rates and the rates of metabolic precursor depletion to biosynthesis, as determined from the composition of P. pastoris biomass previously reported for each oxygenation condition [2], were combined with a set of linearly independent equations obtained from METAFoR analysis to render the complete linear system solvable.
-----------------------------------References---------------------------------
[1] Fischer E, Zamboni N, Sauer U. Anal Biochem. 2004;325(2):308–316. http://doi.org/c6kx35
[2] Carnicer M, Baumann K, Töplitz I, Sánchez-Ferrando F, Mattanovich D, Ferrer P, Albiol J. Microb Cell Fact. 2009;8:65. http://doi.org/b6997b

Data file
Downloadfluxes KIMODATAID114_v1.xlsx
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Related Data: AccessID 55 | AccessID 57 | AccessID 113


Submission and curation info

Entered by Administrator KiMoSysFirst name: Administrator
Affiliation: INESC-ID/IST
Homepage: http://kdbio.inesc-id.pt/kimosys
Interests: mathematical modeling, accessible data, use of data

Created 2018-08-18 22:44:26 UTC

Updated 2018-08-19 15:31:35 UTC

Version 1

Status (reviewed) 2018-08-18 22:45:38 UTC




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