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General Information
| Manuscript title | A multi-level study of recombinant Pichia pastoris in different oxygen conditions. |
| PubMed ID | 20969759 |
| Journal | BMC Systems Biology |
| Year | 2010 |
| Authors | Kristin Baumann, Marc Carnicer, Martin Dragosits, Alexandra B Graf, Johannes Stadlmann, Paula Jouhten, Hannu Maaheimo, Brigitte Gasser, Joan Albiol, Diethard Mattanovich, Pau Ferrer |
| Affiliations | Department of Chemical Engineering, Autonomous University of Barcelona, Spain |
| Keywords | Hypoxic Condition, Unfold Protein Response, Pentose Phosphate Pathway, Metabolic Flux, Flux Ratio |
| Full text article |
Baumann_2010.pdf
|
| Project name | not specified |
Experiment Description
| Organism | Pichia pastoris |
| Strain | X-33/pGAPaA_Fab and X-33/pGAPaA |
| Data type | flux measurements |
| Data units | (mmol/g.h) |
| Execution date | not specified |
Experimental Details
| Temperature (°C) | 25 |
| pH | 5 |
| Carbon source | glucose |
| Culture mode | chemostat |
| Process condition | aerobic |
| Dilution rate (h⁻¹) | 0.1 |
| Working volume (L) | 1.0 |
| Biomass concentration (g/L) | for O2 21% = 0.47 ± 0.02, for O2 11% = 0.45 ± 0.01 and for O2 8% = 0.25 ± 0.01 |
| Medium composition | Minimal medium |
| General protocol information |
Flux analysis method:
flux ratio, 13C constrained MFA Platform: NMR |
| Methods description - Notes | Intracellular metabolic fluxes were determined using 13C-NMR derived flux ratios as additional experimental constraints to solve the MFA system [1]. The complete model for the calculation of intracellular fluxes, comprised 33 (normoxic condition) and 34 (oxygen-limiting and ... Read more |
| Data file |
 KIMODATAID114_v1.xlsx
|
| Alternative format(s) |
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Related Data: AccessID 55 | AccessID 57 | AccessID 113
Submission and curation
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Administrator KiMoSysFirst name: Administrator
Affiliation: INESC-ID/IST
Homepage: http://kdbio.inesc-id.pt/kimosys
Interests: mathematical modeling, accessible data, use of data
Created: 2018-08-18 22:44:26 UTC
Updated: 2020-04-24 16:10:36 UTC
Version: 1
Status: (reviewed) 2018-08-18 22:45:38 UTC
Views: 258
Downloads: 64
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