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General Information
Manuscript title A multi-level study of recombinant Pichia pastoris in different oxygen conditions.
PubMed ID 20969759
Journal BMC Systems Biology
Year 2010
Authors Kristin Baumann, Marc Carnicer, Martin Dragosits, Alexandra B Graf, Johannes Stadlmann, Paula Jouhten, Hannu Maaheimo, Brigitte Gasser, Joan Albiol, Diethard Mattanovich, Pau Ferrer
Affiliations Department of Chemical Engineering, Autonomous University of Barcelona, Spain
Keywords Hypoxic Condition, Unfold Protein Response, Pentose Phosphate Pathway, Metabolic Flux, Flux Ratio
Full text article Baumann_2010.pdf
Project name not specified

Experiment Description
Organism Pichia pastoris
Strain X-33/pGAPaA_Fab and X-33/pGAPaA
Data type flux measurements
Data units (mmol/g.h)
Execution date not specified

Experimental Details
Temperature (°C) 25
pH 5
Carbon source glucose
Culture mode chemostat
Process condition aerobic
Dilution rate (h⁻¹) 0.1
Working volume (L) 1.0
Biomass concentration (g/L) for O2 21% = 0.47 ± 0.02, for O2 11% = 0.45 ± 0.01 and for O2 8% = 0.25 ± 0.01
Medium composition

Minimal medium

General protocol information Flux analysis method: flux ratio, 13C constrained MFA

Platform: NMR

Methods description - Notes

Intracellular metabolic fluxes were determined using 13C-NMR derived flux ratios as additional experimental constraints to solve the MFA system [1]. The complete model for the calculation of intracellular fluxes, comprised 33 (normoxic condition) and 34 (oxygen-limiting and ...

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Entered by: Administrator KiMoSysFirst name: Administrator
Affiliation: INESC-ID/IST
Interests: mathematical modeling, accessible data, use of data

Created: 2018-08-18 22:44:26 UTC

Updated: 2020-04-24 16:10:36 UTC

Version: 1

Status: (reviewed) 2018-08-18 22:45:38 UTC

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Downloads: 26

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