DataEntryID 114 General information Manuscript title: A multi-level study of recombinant Pichia pastoris in different oxygen conditions. PubMed ID: http://www.ncbi.nlm.nih.gov/pubmed/20969759 Journal: BMC Systems Biology Year: 2010 Authors: Kristin Baumann, Marc Carnicer, Martin Dragosits, Alexandra B Graf, Johannes Stadlmann, Paula Jouhten, Hannu Maaheimo, Brigitte Gasser, Joan Albiol, Diethard Mattanovich, Pau Ferrer Affiliations: Department of Chemical Engineering, Autonomous University of Barcelona, Spain Keywords: Hypoxic Condition, Unfold Protein Response, Pentose Phosphate Pathway, Metabolic Flux, Flux Ratio Full text article: https://kimosys.org/rails/active_storage/blobs/eyJfcmFpbHMiOnsibWVzc2FnZSI6IkJBaHBBdGtFIiwiZXhwIjpudWxsLCJwdXIiOiJibG9iX2lkIn19--ecd2dc16b2914a374327bd3fd2f4a2bdd93f7326/Baumann_2010.pdf Project name: not specified Experiment description Organism: Pichia pastoris Strain: X-33/pGAPaA_Fab and X-33/pGAPaA Data type: flux measurements Data units: (mmol/g.h) Execution date: not specified Experimental details Temperature (°C): 25 pH: 5 Carbon source: glucose Culture mode: chemostat Process condition: aerobic Dilution rate (h⁻¹): 0.1 Working volume: 1.0 L Biomass concentration (g/L): for O2 21% = 0.47 ± 0.02, for O2 11% = 0.45 ± 0.01 and for O2 8% = 0.25 ± 0.01 Medium composition: Minimal medium General protocol information: Type analysis list: flux ratio, 13C constrained MFA; Platform list: NMR; Methods description: Intracellular metabolic fluxes were determined using 13C-NMR derived flux ratios as additional experimental constraints to solve the MFA system [1]. The complete model for the calculation of intracellular fluxes, comprised 33 (normoxic condition) and 34 (oxygen-limiting and hypoxic conditions) metabolic reactions. The measured uptake and excretion rates and the rates of metabolic precursor depletion to biosynthesis, as determined from the composition of P. pastoris biomass previously reported for each oxygenation condition [2], were combined with a set of linearly independent equations obtained from METAFoR analysis to render the complete linear system solvable. -----------------------------------References--------------------------------- [1] Fischer E, Zamboni N, Sauer U. Anal Biochem. 2004;325(2):308–316. http://doi.org/c6kx35 [2] Carnicer M, Baumann K, Töplitz I, Sánchez-Ferrando F, Mattanovich D, Ferrer P, Albiol J. Microb Cell Fact. 2009;8:65. http://doi.org/b6997b Data file: http://kimosys.org/repository/114/download?parameter=1240; Alternative formats: no files uploaded Submission and curation Entered by: Administrator KiMoSys Created: 2018-08-18 22:44:26 UTC Updated: 2020-04-24 16:10:36 UTC Version: 1 Status: (reviewed) 2018-08-18 22:45:38 UTC Views: 216 Downloads: 56