DataEntryID 114
    General information
      Manuscript title: A multi-level study of recombinant Pichia pastoris in different oxygen conditions.
      PubMed ID: http://www.ncbi.nlm.nih.gov/pubmed/20969759
      Journal: BMC Systems Biology
      Year: 2010
      Authors: Kristin Baumann, Marc Carnicer, Martin Dragosits, Alexandra B Graf, Johannes Stadlmann, Paula Jouhten, Hannu Maaheimo, Brigitte Gasser, Joan Albiol, Diethard Mattanovich, Pau Ferrer
      Affiliations: Department of Chemical Engineering, Autonomous University of Barcelona, Spain
      Keywords: Hypoxic Condition, Unfold Protein Response, Pentose Phosphate Pathway, Metabolic Flux, Flux Ratio
      Full text article: https://kimosys.org/rails/active_storage/blobs/eyJfcmFpbHMiOnsibWVzc2FnZSI6IkJBaHBBdGtFIiwiZXhwIjpudWxsLCJwdXIiOiJibG9iX2lkIn19--ecd2dc16b2914a374327bd3fd2f4a2bdd93f7326/Baumann_2010.pdf
      Project name: not specified

    Experiment description
      Organism: Pichia pastoris
      Strain: X-33/pGAPaA_Fab and X-33/pGAPaA
      Data type: flux measurements
      Data units: (mmol/g.h)
      Execution date: not specified

    Experimental details
      Temperature (°C): 25
      pH: 5
      Carbon source: glucose
      Culture mode: chemostat
      Process condition: aerobic
      Dilution rate (h⁻¹): 0.1
      Working volume: 1.0 L
      Biomass concentration (g/L): for O2 21% = 0.47 ± 0.02, for O2 11% = 0.45 ± 0.01 and for O2 8% = 0.25 ± 0.01
      Medium composition: Minimal medium
      General protocol information: Type analysis list: flux ratio, 13C constrained MFA; Platform list: NMR;
      Methods description: Intracellular metabolic fluxes were determined using 13C-NMR derived flux ratios as additional experimental constraints to solve the MFA system [1]. The complete model for the calculation of intracellular fluxes, comprised 33 (normoxic condition) and 34 (oxygen-limiting and hypoxic conditions) metabolic reactions. The measured uptake and excretion rates and the rates of metabolic precursor depletion to biosynthesis, as determined from the composition of P. pastoris biomass previously reported for each oxygenation condition [2], were combined with a set of linearly independent equations obtained from METAFoR analysis to render the complete linear system solvable.
-----------------------------------References---------------------------------
[1] Fischer E, Zamboni N, Sauer U. Anal Biochem. 2004;325(2):308–316. http://doi.org/c6kx35
[2] Carnicer M, Baumann K, Töplitz I, Sánchez-Ferrando F, Mattanovich D, Ferrer P, Albiol J. Microb Cell Fact. 2009;8:65. http://doi.org/b6997b
      Data file: http://kimosys.org/repository/114/download?parameter=1240; 
      Alternative formats: no files uploaded

    Submission and curation
      Entered by: Administrator KiMoSys
      Created: 2018-08-18 22:44:26 UTC
      Updated: 2020-04-24 16:10:36 UTC
      Version: 1
      Status: (reviewed) 2018-08-18 22:45:38 UTC
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      Downloads: 48