A multi-level study of recombinant Pichia pastoris in different oxygen conditions.
http://www.ncbi.nlm.nih.gov/pubmed/20969759
BMC Systems Biology
2010
Kristin Baumann, Marc Carnicer, Martin Dragosits, Alexandra B Graf, Johannes Stadlmann, Paula Jouhten, Hannu Maaheimo, Brigitte Gasser, Joan Albiol, Diethard Mattanovich, Pau Ferrer
Department of Chemical Engineering, Autonomous University of Barcelona, Spain
Hypoxic Condition, Unfold Protein Response, Pentose Phosphate Pathway, Metabolic Flux, Flux Ratio
https://kimosys.org/rails/active_storage/blobs/eyJfcmFpbHMiOnsibWVzc2FnZSI6IkJBaHBBdGtFIiwiZXhwIjpudWxsLCJwdXIiOiJibG9iX2lkIn19--ecd2dc16b2914a374327bd3fd2f4a2bdd93f7326/Baumann_2010.pdf
not specified
Pichia pastoris
X-33/pGAPaA_Fab and X-33/pGAPaA
flux measurements
(mmol/g.h)
not specified
25 °C
5
glucose
chemostat
aerobic
0.1 h⁻¹
1.0 L
for O2 21% = 0.47 ± 0.02, for O2 11% = 0.45 ± 0.01 and for O2 8% = 0.25 ± 0.01 g/L
Minimal medium
Type analysis list: flux ratio, 13C constrained MFA; Platform list: NMR;
Intracellular metabolic fluxes were determined using 13C-NMR derived flux ratios as additional experimental constraints to solve the MFA system [1]. The complete model for the calculation of intracellular fluxes, comprised 33 (normoxic condition) and 34 (oxygen-limiting and hypoxic conditions) metabolic reactions. The measured uptake and excretion rates and the rates of metabolic precursor depletion to biosynthesis, as determined from the composition of P. pastoris biomass previously reported for each oxygenation condition [2], were combined with a set of linearly independent equations obtained from METAFoR analysis to render the complete linear system solvable.
-----------------------------------References---------------------------------
[1] Fischer E, Zamboni N, Sauer U. Anal Biochem. 2004;325(2):308–316. http://doi.org/c6kx35
[2] Carnicer M, Baumann K, Töplitz I, Sánchez-Ferrando F, Mattanovich D, Ferrer P, Albiol J. Microb Cell Fact. 2009;8:65. http://doi.org/b6997b
http://kimosys.org/repository/114/download?parameter=1240;
Administrator KiMoSys
2018-08-18 22:44:26 UTC
2020-04-24 16:10:36 UTC
1
(reviewed) 2018-08-18 22:45:38 UTC
186
45