M9 minimal medium (per liter): 8.5 g of Na2HPO4.2H20, 3 g of KH2PO4, 1 g of NH4Cl, 0.5 g of NaCl. The following components were sterilized separately and then added (per liter of final medium): 1 ml of 0.1 m CaCl2.2H2O, 1 ml of 1 m MgSO4.7H2O, 1 ml of 50 mm FeCl3.6H2O, and 10 ml of trace salt solution. The trace salts solution contained (per liter): 170 mg of ZnCl2, 100 mg of MnCl2.4H2O, 60.0 mg of CoCl2.6H2O, 60.0 mg of Na2MoO4.2H2O, and 43.0 mg CuCl2.2H2O. When preparing the medium, the base salts were added first followed by CalCl2, MgSO4, FeCl3, and finally the trace elements.

GC-TOF Workflow: Dried aliquots were derivatized with 15 μl of either TMS-reagent (N-methyl-N-(trimethylsilyl)trifluoroacetamide, Fluka, Buchs, Switzerland) or TBDMS reagent (N-tert-butyldimethylsilyl-N-methyltrifluoroacetamide, Fluka), and aliquots of 5 μl were injected int ... o a 30-m GC column (HP-5-MS, 30 m × 0.25 mm × 0.25 μm, Agilent) using a CIS4 injector (Gerstel, Mülheim an der Ruhr, Germany) and solvent venting. Derivatized metabolites were detected on a Pegasus 3D TOF mass spectrometer (Leco), with an acquisition rate of 40 Hz. Peak detection and assignment were performed with ChromaTOF software (Version 2.32, Leco). Liquid Chromatography-MS/MS Workflow: Dry metabolite extracts were resuspended in 100 μl of water, 8 μl of which were injected on a Agilent 1100 series HPLC stack with a Synergi Hydro RP 2.1 × 150 × 4 column (Phenomenex, Aschaffenburg, Germany). Metabolites were detected using a 4000 QTRAP mass spectrometer (AB/MDS Sciex, Concord, Canada) operated in tandem MS mode with unit mass resolution. Analyst software (Version 1.4.2, AB/MDS Sciex) was used for both acquisition and integration. Ion spray voltage, auxiliary gas temperature, nebulizer gas, auxiliary gas, curtain gas, and collision gas were set to −4200 V, 650 °C, and 65, 40, 10, 4 (arbitrary units), respectively.
---------References---------
[1] Ewald J. C., Heux S., Zamboni N. (2009) Anal. Chem. 81, 3623–3629. http://doi.org/chgtcq [2] Büscher J. M., Czernik D., Ewald J. C., Sauer U., Zamboni N. (2009) Anal. Chem. 81, 2135–2143. http://doi.org/cjqg95