Detail View - Data AccessID 88
General Information
Manuscript title | Transcriptional regulation is insufficient to explain substrate-induced flux changes in Bacillus subtilis |
PubMed ID | 24281055 |
Journal | Molecular Systems Biology |
Year | 2013 |
Authors | Victor Chubukov, Markus Uhr, Ludovic Le Chat, Roelco J Kleijn, Matthieu Jules, Hannes Link, Stephane Aymerich, Joerg Stelling and Uwe Sauer |
Affiliations | Institute of Molecular System Biology, ETH Zurich, Zurich, Switzerland. |
Keywords | central carbon metabolism, metabolic flux, transcriptional regulation |
Full text article | Chubukov_2013.pdf |
Project name | not specified |
Experiment Description
Organism | Bacillus subtilis |
Strain | BSB168 |
Data type | metabolites at steady-state |
Data units | mMol/L cell volume |
Execution date | not specified |
Experimental Details
Temperature (°C) | 37.0 |
pH | — |
Carbon source | glucose, fructose, gluconate, succinate+glutamate, glycerol, malate, malate, malate+glucose, pyruvate |
Culture mode | batch |
Process condition | aerobic |
Dilution rate (h⁻¹) | — |
Working volume (L) | 0.03 |
Biomass concentration (g/L) | 3.0 g cells/OD600 |
Medium composition | M9 minimal medium (per liter): 8.5 g Na2HPO4·2H2O, 3 g KH2PO4, 1 g NH4Cl, 0.5 g NaCl. The following components were sterilized separately and then added (per liter of final medium): 1 ml 0.1 M CaCl2, 1 ml 1 M MgSO4, 1 ml 50 mM FeCl3 and 10 ml trace salts solution. The trace salts solution contained (per liter): 170 mg ZnCl2, 100 mg MnCl2·4H2O, 60.0 mg CoCl2·6H2O, 60.0 mg Na2MoO4·2H2O and 43.0 mg CuCl2·2H2O. Filter‐sterilized carbon sources were added separately to the medium, pH neutralized with 4 M NaOH where necessary. |
General protocol information |
Sampling method:
Sampling method: Two samples for intracellular metabolite quantification were taken within 5 min of each other from the shake flask cultures during exponential growth at an OD600 between 0.8 and 1.2. Quenching procedure: — Extraction technique: hot ethanol Sample analyzing method: HPLC-MS |
Methods description - Notes | In all, 2 ml of culture was vacuum filtered on a 0.45‐μm pore size nitrocellulose filter (Millipore) and immediately washed with two volumes of fresh M9 medium containing the respective carbon source and adjusted to the pH of the culture at the time of sampling. Sampling was ... Read more |
Data file |
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Related Data: AccessID 89 | AccessID 94 | AccessID 95
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Administrator KiMoSysFirst name: Administrator
Affiliation: INESC-ID/IST
Homepage: http://kdbio.inesc-id.pt/kimosys
Interests: mathematical modeling, accessible data, use of data
Created: 2015-05-05 16:47:23 UTC
Updated: 2020-04-24 16:10:35 UTC
Version: 3
Status: (reviewed) 2015-05-05 16:47:54 UTC
Views: 232
Downloads: 64
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