not specified

Human DNL measurements. Human subjects (n=4) were studied in compliance with and approval by the UT Southwestern Institutional Review Board. All participants were provided written informed consent before participation. Healthy volunteers were studied after a self-managed ove ... rnight fast. A baseline blood sample was drawn prior to 2H2O administration. Subjects consumed a single oral dose of 70% 2H2O at 2 g/kg body-water (where body-water was estimated to be 0.6 x body weight in men and 0.5 x body-weight in women), or roughly ~1g/Kg body weight. Blood samples were collected at 0.5, 1, 1.5 and 2-hours. Following the 2-hour collection, subjects consumed a liquid meal consisting of 8 ounces of Ensure containing 220 Kcal, 6 g fat, 32 g carbohydrates, 15 g sugar and 9 g protein (Abbot Labs, Columbus OH). Blood collections continued at 2.5, 3, 3.5 and 4-hours.