DataEntryID 130
    General information
      Manuscript title: Measurement of Lipogenic Flux by Deuterium Resolved Mass Spectrometry
      PubMed ID: http://www.ncbi.nlm.nih.gov/pubmed/not specified
      Journal: Nature Communication
      Year: 2021
      Authors: Xiaorong Fu, Stanisław Deja, Justin A. Fletcher, Norma N. Anderson, Monika Mizerska, Gonçalo Vale, Jeffrey D Browning, Jay D. Horton, Jeffrey G. McDonald, Matthew A. Mitsche, and Shawn C. Burgess
      Affiliations: UT Southwestern Medical Center
      Keywords: Lipogenesis, Liver, palmitate, fatty acid synthase, deuterated water, deuterium, orbitrap, mass spectrometry, human
      Full text article: no file uploaded
      Project name: DNL

    Experiment description
      Organism: homo sapiens
      Strain: Normal volunteers
      Data type: flux measurements
      Data units: fractional dnl
      Execution date: 0005-05-21

    Experimental details
      Temperature (°C): 37
      pH: 7.4
      Carbon source: deuterated water
      Culture mode: batch
      Process condition: not specified
      Dilution rate (h⁻¹): not specified
      Working volume: not specified L
      Biomass concentration (g/L): not specified
      Medium composition: not specified
      General protocol information: Type analysis list: not specified; Platform list: not specified;
      Methods description: Human DNL measurements. Human subjects (n=4) were studied in compliance with and approval by the UT Southwestern Institutional Review Board. All participants were provided written informed consent before participation. Healthy volunteers were studied after a self-managed overnight fast. A baseline blood sample was drawn prior to 2H2O administration. Subjects consumed a single oral dose of 70% 2H2O at 2 g/kg body-water (where body-water was estimated to be 0.6 x body weight in men and 0.5 x body-weight in women), or roughly ~1g/Kg body weight. Blood samples were collected at 0.5, 1, 1.5 and 2-hours. Following the 2-hour collection, subjects consumed a liquid meal consisting of 8 ounces of Ensure containing 220 Kcal, 6 g fat, 32 g carbohydrates, 15 g sugar and 9 g protein (Abbot Labs, Columbus OH). Blood collections continued at 2.5, 3, 3.5 and 4-hours.
      Data file: http://kimosys.org/repository/130/download?parameter=1585; http://kimosys.org/repository/130/download?parameter=1584; 
      Alternative formats: no files uploaded

    Submission and curation
      Entered by: Shawn Burgess
      Created: 2021-05-05 13:30:43 UTC
      Updated: 2021-05-05 13:31:16 UTC
      Version: 1
      Status: (unreviewed)
      Views: 95
      Downloads: 114