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Manuscript title Metabonomic profiles delineate potential role of glutamate-glutamine cycle in db/db mice with diabetes-associated cognitive decline
PubMed ID not specified
Journal Molecular brain
Year 2016
Authors Yongquan Zheng, Yunjun Yang, Baijun Dong, Hong Zheng, Xiaodong Lin, Du Yao, Xiaokun Li, Liangcai Zhao, Hongchang Gao
Affiliations Radiology Department of the First Affiliated Hospital, Wenzhou Medical University;School of Pharmaceutical Sciences, Wenzhou Medical University, Department of Urology, Renji Hospital, Shanghai Jiao Tong University School of Medicine.
Keywords Diabetes-associated cognition decline, Nuclear magnetic resonance, Metabonomics, Glutamate-glutamine cycle
Full text article Downloadarticle Zheng_2016.pdf
Project name not specified

Experiment Description info

Organism Rattus
Strain C57BLKS/J-m+/+db and mutants
Data type metabolites at steady-state
Data units unitless
Execution date not specified

Experimental Details info

Temperature (0C) 25
pH 7
Carbon source glucose,
Culture mode batch
Process condition aerobic
Dilution rate (h-1) not specified
Working volume (L) 0.0005
Biomass concentration (g/L) not specified
Medium composition

PBS

General protocol information Sampling method: The mice were sacrificed by decapitation at 17-wk of age, and specimens of hippocampus were collected manually.

Quenching procedure: specimens of hippocampus were dissected immediately, snap-frozen in liquid nitrogen and stored at -80 °C until use.

Extraction technique: chloroform

Sample analyzing method: NMR

Methods description - Notes

The preparation of the brain extracts was based on the previous reference [1]. The frozen tissue was weighed and ground using an electric homogenizer with ice-cold methanol (4 mL/g) and distilled water (0.85 mL/g) at 4°C and the mixture was vortexed. Chloroform (2 mL/g) and distilled water (2 mL/g) was added and mixed again. After keeping on ice for 15 min, the homogenate was centrifuged at 1,000 g for 15 min at 4°C. The supernatant was extracted and lyophilized for about 24h. The metabolite mixture obtained was then weighed and dissolved in 0.6 mL of 99.5% D2O for NMR spectroscopy. One-dimensional ZGPR pulse sequence was used, and the main parameters were set as follows: data points, 64 K; spectral width, 12,000 Hz; relaxation delay, 10 s. The spectral segments for each NMR spectrum were normalized to the total sum of the spectral intensity to partially compensate for differences in concentration of the many metabolites in the samples [2].
--------------References--------------
[1] Gao H, Xiang Y, Sun N, Zhu H, Wang Y, Liu M, Ma Y, Lei H: Metabolic changes in rat prefrontal cortex and hippocampus induced by chronic morphine treatment studied ex vivo by high resolution 1H NMR spectroscopy. Neurochem Int 2007, 50:386-394. http://doi.org/ds7tm7
[2] Coen M, Lenz EM, Nicholson JK, Wilson ID, Pognan F, Lindon JC: An integrated metabonomic investigation of acetaminophen toxicity in the mouse using NMR spectroscopy. Chem Res Toxicol 2003, 16:295-303. http://doi.org/bhqmgj

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Submission and curation info

Entered by Administrator KiMoSysFirst name: Administrator
Affiliation: INESC-ID/IST
Homepage: http://kdbio.inesc-id.pt/kimosys
Interests: mathematical modeling, accessible data, use of data

Created 2016-03-25 16:53:20 UTC

Team Liangcai ZhaoFirst name: Liangcai
Affiliation: Wenzhou Medical University
Homepage:
Interests:

Updated 2016-04-26 15:38:14 UTC

Version 2

Status (reviewed) 2016-03-29 12:07:02 UTC




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