DataEntryID 91
    General information
      Manuscript title: Metabonomic profiles delineate potential role of glutamate-glutamine cycle in db/db mice with diabetes-associated cognitive decline
      PubMed ID: http://www.ncbi.nlm.nih.gov/pubmed/not specified
      Journal: Molecular brain
      Year: 2016
      Authors: Yongquan Zheng, Yunjun Yang, Baijun Dong, Hong Zheng, Xiaodong Lin, Du Yao, Xiaokun Li, Liangcai Zhao, Hongchang Gao
      Affiliations: Radiology Department of the First Affiliated Hospital, Wenzhou Medical University;School of Pharmaceutical Sciences, Wenzhou Medical University, Department of Urology, Renji Hospital, Shanghai Jiao Tong University School of Medicine.
      Keywords: Diabetes-associated cognition decline, Nuclear magnetic resonance, Metabonomics, Glutamate-glutamine cycle
      Full text article: https://kimosys.org/rails/active_storage/blobs/eyJfcmFpbHMiOnsibWVzc2FnZSI6IkJBaHBBcThFIiwiZXhwIjpudWxsLCJwdXIiOiJibG9iX2lkIn19--f744d8c9f07f13e33dc87f1a4ee42a8851e12aa6/Zheng_2016.pdf
      Project name: not specified

    Experiment description
      Organism: Rattus
      Strain: C57BLKS/J-m+/+db and mutants
      Data type: metabolites at steady-state
      Data units: unitless
      Execution date: not specified

    Experimental details
      Temperature (°C): 25
      pH: 7
      Carbon source: glucose
      Culture mode: batch
      Process condition: aerobic
      Dilution rate (h⁻¹): not specified
      Working volume: 0.0005 L
      Biomass concentration (g/L): not specified
      Medium composition: PBS
      General protocol information: Sampling Method:  The mice were sacrificed by decapitation at 17-wk of age, and specimens of hippocampus were collected manually.; Quenching: specimens of hippocampus were dissected immediately, snap-frozen in liquid nitrogen and stored at -80 °C until use.; Extraction list: chloroform; Analysis list: NMR;
      Methods description: The preparation of the brain extracts was based on the previous reference [1]. The frozen tissue was weighed and ground using an electric homogenizer with ice-cold methanol (4 mL/g) and distilled water (0.85 mL/g) at 4°C and the mixture was vortexed. Chloroform (2 mL/g) and distilled water (2 mL/g) was added and mixed again. After keeping on ice for 15 min, the homogenate was centrifuged at 1,000 g for 15 min at 4°C. The supernatant was extracted and lyophilized for about 24h. The metabolite mixture obtained was then weighed and dissolved in 0.6 mL of 99.5% D2O for NMR spectroscopy. One-dimensional ZGPR pulse sequence was used, and the main parameters were set as follows: data points, 64 K; spectral width, 12,000 Hz; relaxation delay, 10 s.  The spectral segments for each NMR spectrum were normalized to the total sum of the spectral intensity to partially compensate for differences in concentration of the many metabolites in the samples [2].                                                                                                                                                                                                                         
                                                   --------------References--------------                                                                                                                                                                              
[1] Gao H, Xiang Y, Sun N, Zhu H, Wang Y, Liu M, Ma Y, Lei H: Metabolic changes in rat prefrontal cortex and hippocampus induced by chronic morphine treatment studied ex vivo by high resolution 1H NMR spectroscopy. Neurochem Int 2007, 50:386-394. http://doi.org/ds7tm7                                                                                                                                                                                                       
[2] Coen M, Lenz EM, Nicholson JK, Wilson ID, Pognan F, Lindon JC: An integrated metabonomic investigation of acetaminophen toxicity in the mouse using NMR spectroscopy. Chem Res Toxicol 2003, 16:295-303. http://doi.org/bhqmgj
      Data file: http://kimosys.org/repository/91/download?parameter=1198; 
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    Submission and curation
      Entered by: Administrator KiMoSys
      Created: 2016-03-25 16:53:20 UTC
      Updated: 2020-04-24 16:10:35 UTC
      Version: 2
      Status: (reviewed) 2016-03-29 12:07:02 UTC
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      Downloads: 53