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General Information
Manuscript title Division of labor by dual feedback regulators controls JAK2/STAT5 signaling over broad ligand range.
PubMed ID 21772264
Journal Molecular Systems Biology
Year 2011
Authors Julie Bachmann, Andreas Raue, Marcel Schilling, Martin E. Boehm, Clemens Kreutz, Daniel Kaschek, Hauke Busch, Norbert Gretz, Wolf D. Lehmann, Jens Timmer and Ursula Klingmueller
Affiliations Division of Systems Biology of Signal Transduction, DKFZ-ZMBH Alliance, German Cancer Research Center, Heidelberg, Germany
Keywords apoptosis, erythropoietin, mathematical modeling, negative feedback, SOCS, simulation and data analysis, signal transduction
Full text article Bachmann_2011.pdf
Project name not specified


Experiment Description
Organism Mus musculus
Strain E13.5 Balb/c
Data type enzyme/protein concentrations
Data units (a.u.)
Execution date not specified


Experimental Details
Temperature (°C) 37.0
pH not specified
Carbon source not specified
Culture mode batch
Process condition not specified
Dilution rate (h⁻¹)
Working volume (L) not specified
Biomass concentration (g/L) 4.0×10^7 (cells/L)
Medium composition

Cultivated for 12–14 h in IMDM (Invitrogen), 30% fetal calf serum (FCS), and 50 μM β-mercaptoethanol supplemented with 0.5 U/ml Epo (Cilag-Jansen).

General protocol information Measurement method: immunoblotting

Methods description - Notes

Time course experiments, cell lysis and quantitative immunoblotting - erythroid progenitor cells were washed three times in medium and starved in Panserin 401 (Pan Biotech) supplemented with 50 µM ß-mercaptoethanol and 1 mg/ml BSA (Sigma-Aldrich) for 1 h. Subsequently, cells ...

---------------------References------------------
[1] Ketteler R, Moghraby CS, Hsiao JG, Sandra O, Lodish HF, Klingmuller U(2003). The cytokine-inducible Scr homology domain-containing protein negatively regulates signaling by promoting apoptosis in erythroid progenitor cells. J Biol Chem 278: 2654–2660. http://doi.org/b6fp9v
[2] Schilling M, Maiwald T, Bohl S, Kollmann M, Kreutz C, Timmer J, Klingmuller U (2005) Quantitative data generation for systems biology: the impact of randomisation, calibrators and normalisers. Syst Biol (Stevenage) 152: 193–200. http://doi.org/dknn3j
[3] Klingmuller U, Lorenz U, Cantley LC, Neel BG, Lodish HF (1995). Specific recruitment of SH-PTP1 to the erythropoietin receptor causes inactivation of JAK2 and termination of proliferative signals. Cell 80: 729–738. http://dx.doi.org/10.1016/0092-8674(95)90351-8

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Submission and curation

Entered by: Administrator KiMoSysFirst name: Administrator
Affiliation: INESC-ID/IST
Homepage: http://kdbio.inesc-id.pt/kimosys
Interests: mathematical modeling, accessible data, use of data

Created: 2014-04-23 15:16:48 UTC

Updated: 2020-04-24 16:10:34 UTC

Version: 0

Status: (reviewed) 2018-07-07 21:38:44 UTC

Views: 23

Downloads: 1




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EntryID
Model name Category Model Type Data used for Access Json
31 transcriptional model of JAK2/STAT5 signaling Signal ordinary differential equations Model building {"id":31,"organism_id":null,"comments":"","sbml_file_name":"Bachmann_2011.pdf","article_file_name":null,"category":"Signal","used_for":"---\n- Model building\n","model_type":"ordinary differential equations","pubmed_id":"21772264","dilution_rate":"","name_of_model":"transcriptional model of JAK2/STAT5 signaling","manuscript_title":"Division of labor by dual feedback regulators controls JAK2/STAT5 signaling over broad ligand range.","authors":"Julie Bachmann, Andreas Raue, Marcel Schilling, Martin E. Boehm, Clemens Kreutz, Daniel Kaschek, Hauke Busch, Norbert Gretz, Wolf D. Lehmann, Jens Timmer and Ursula Klingmueller","journal":"Molecular Systems Biology","affiliation":"Division of Systems Biology of Signal Transduction, DKFZ-ZMBH Alliance, German Cancer Research Center, Heidelberg, Germany","project_name":"","biomodels_id":"","keywords":"apoptosis, erythropoietin, mathematical modeling, negative feedback, SOCS, simulation and data analysis, signal transduction","software":"http://www.matlab.com (MATLAB)","control":null,"main_organism":"Mouse","year":2011,"combine_archive_file_name":"COMBINE_KIMOMODELID31.omex","combine_archive_content_type":"application/omex+xml","combine_archive_file_size":1445888,"combine_archive_updated_at":"2014-06-30T17:59:26.698Z","review_journal_id":null,"doi":null} Administrator KiMoSys



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