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General Information
Manuscript title Is the glycolytic flux in Lactococcus lactis primarily controlled by the redox charge? Kinetics of NAD(+) and NADH pools determined in vivo by 13C NMR.
PubMed ID 12011086
Journal The Journal of Biological Chemistry
Year 2002
Authors Ana Rute Neves, Rita Ventura, Nahla Mansour, Claire Shearman, Michael J. Gasson, Christopher Maycock, Ana Ramos and Helena Santos
Affiliations Instituto de Teconcologia Química e Biológica, Universidade Nova de Lisboa and Instituto de Biologia Experimental e Tecnológico, Oeiras, Portugal
Keywords Lactococcus lactis, metabolism of glucose, NMR, in vivo
Full text article Neves_2002.pdf
Project name PneumoSyS

Experiment Description
Organism Lactococcus lactis
Strain MG1363
Data type time-series data of metabolites
Data units not specified
Execution date not specified

Experimental Details
Temperature (°C) 30.0
pH 6.5
Carbon source glucose
Culture mode batch
Process condition anaerobic
Dilution rate (h⁻¹) not specified
Working volume (L) 0.050
Biomass concentration (g/L) not specified
Medium composition

Chemical-defined medium

General protocol information Sampling method: Cells were harvested in logarithmic growth phase (A 600 = 2.2)

Quenching procedure: Centrifuges, washed, and suspended to a protein concentration of 16,5 mg/ml in 50mM Kpi or Mes/KOA buffer, pH 6,5, for 13C or 31P NMR respectively.

Extraction technique: boiling ethanol

Sample analyzing method: NMR

Methods description - Notes

NMR Experiments - cells were harvested in mid-logarithmic growth phase (A600 = 2.2), centrifuged, washed twice, and suspended to a protein concentration of 16.5 mg/ml in 50 mM KPi or Mes/KOH buffer, pH 6.5, for 13C or 31P NMR experiments, respectively.
Quantification o ...

[1] de Vos, W.M (1999). Curr. Opin, Microbiol. 2, 289-295.
[2] Neves, A. R., Ramos, A., Nunes, M. C., Kleerebezem, M., Hugenholtz, J., de Vos, W. M., Almeida, J., and Santos, H. (1999). Biotechnol. Bioeng. 64, 200–212.
[3] Poolman, B., Smid, E. J., Veldkamp, H., and Konings, W. N. (1987). J. Bacteriol. 169, 1460–1468.

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Related Data: AccessID 53

Submission and curation

Entered by: Administrator KiMoSysFirst name: Administrator
Affiliation: INESC-ID/IST
Interests: mathematical modeling, accessible data, use of data

Created: 2013-04-24 13:08:01 UTC

Updated: 2020-04-24 16:10:29 UTC

Version: 1

Status: (reviewed) 2014-05-01 22:49:12 UTC

Views: 291

Downloads: 42

Associated Models

Here we can find relevant models associated with Data EntryID 37:

Model name Category Model Type Data used for Access Json
23 glycolysis Lactococcus lactis Metabolism ordinary differential equations Model building and Model validation {"id":23,"organism_id":37,"comments":"Original model source: in JWS online database.","sbml_file_name":"Levering_2012.pdf","article_file_name":null,"category":"Metabolism","used_for":"---\n- Model building\n- Model validation\n","model_type":"ordinary differential equations","pubmed_id":"22325620","dilution_rate":"","name_of_model":"glycolysis Lactococcus lactis","manuscript_title":"Role of phosphate in the central metabolism of two lactic acid bacteria - a comparative systems biology approach.","authors":"Jennifer Levering, Mark W. J. M. Musters, Martijn Bekker, Domenico Bellomo, Tomas Fiedler, Willem M. de Vos, Jeroen Hugenholtz, Bernd Kreikemeyer, Ursula Kummer and Bas Teusink","journal":"FEBS Journal","affiliation":"Department of Modeling of Biological Processes, COS Heidelberg ⁄ BIOQUANT, University of Heidelberg, Germany","project_name":"","biomodels_id":"levering1","keywords":"Lactococcus lactis, metabolism of glucose, NMR, in vivo","software":" (COPASI)","control":"2014-03-10T11:18:15.244Z","main_organism":"Lactococcus lactis","year":2012,"combine_archive_file_name":"COMBINE_KIMOMODELID23.omex","combine_archive_content_type":"application/omex+xml","combine_archive_file_size":1068365,"combine_archive_updated_at":"2014-06-30T17:53:53.205Z","review_journal_id":null,"doi":null} Administrator KiMoSys
24 DEB L. lactis model Metabolism ordinary differential equations Model building {"id":24,"organism_id":37,"comments":"Archive containing all files to run and plot simulations. Requires MATLAB.","sbml_file_name":"Vinga_2010.pdf","article_file_name":null,"category":"Metabolism","used_for":"---\n- Model building\n","model_type":"ordinary differential equations","pubmed_id":"20921043","dilution_rate":"","name_of_model":"DEB L. lactis model","manuscript_title":"Subcellular metabolic organization in the context of dynamic energy budget and biochemical systems theories","authors":"Susana Vinga, Ana R. Neves, Helena Santos, B. W. Brandt and S. A. L. M. Kooijman","journal":"Philosophical transactions of the royal society B","affiliation":"Instituto de Engenharia de Sistemas e Computadores: Investigação e Desenvolvimento (INESC-ID)","project_name":"","biomodels_id":"","keywords":"Lactococcus lactis, metabolism of glucose, NMR, in vivo","software":" (MATLAB)","control":"2014-05-15T09:26:26.874Z","main_organism":"Lactococcus lactis","year":2010,"combine_archive_file_name":null,"combine_archive_content_type":null,"combine_archive_file_size":null,"combine_archive_updated_at":null,"review_journal_id":null,"doi":null} Administrator KiMoSys
35 glycolysis Lactococcus lactis for mannitol and 2,3-butanediol production Metabolism ordinary differential equations Model building and Model validation {"id":35,"organism_id":null,"comments":" Copasi model file used to produce manuscript figures.","sbml_file_name":"Costa_2014.pdf","article_file_name":null,"category":"Metabolism","used_for":"---\n- Model building\n- Model validation\n","model_type":"ordinary differential equations","pubmed_id":"24413179","dilution_rate":"—","name_of_model":"glycolysis Lactococcus lactis for mannitol and 2,3-butanediol production","manuscript_title":"An extended dynamic model of Lactococcus lactis metabolism for mannitol and 2,3-butanediol production","authors":"Rafael S. Costa, Andras Hartmann, Paula Gaspar, Ana R. Neves and Susana Vinga","journal":"Molecular BioSystems","affiliation":"Instituto de Engenharia de Sistemas e Computadores, Investigacão e Desenvolvimento (INESC-ID), R Alves Redol 9, 1000-029 Lisboa, Portugal.","project_name":"PneumoSyS","biomodels_id":"","keywords":"L. lactis, extended model, mannitol and 2,3-butanediol production, control analysis","software":" (COPASI)","control":"2014-07-21T10:18:41.595Z","main_organism":"Lactococcus lactis","year":2014,"combine_archive_file_name":null,"combine_archive_content_type":null,"combine_archive_file_size":null,"combine_archive_updated_at":null,"review_journal_id":null,"doi":null} Rafael Costa

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