DataEntryID 93
    General information
      Manuscript title: Differential glucose repression in common yeast strains in response to HXK2 deletion.
      PubMed ID: http://www.ncbi.nlm.nih.gov/pubmed/20199578
      Journal: FEMS Yeast Research
      Year: 2010
      Authors: Anne Kümmel, Jennifer Christina Ewald, Sarah‐Maria Fendt, Stefan Jasper Jol, Paola Picotti, Ruedi Aebersold, Uwe Sauer, Nicola Zamboni, Matthias Heinemann
      Affiliations: Institute of Molecular Systems Biology, ETH Zurich
      Keywords: glucose repression; hexokinase 2; FY4; CEN.PK; PKA; metabolomics
      Full text article: https://kimosys.org/rails/active_storage/blobs/eyJfcmFpbHMiOnsibWVzc2FnZSI6IkJBaHBBcklFIiwiZXhwIjpudWxsLCJwdXIiOiJibG9iX2lkIn19--85fd6ba3f9b95fd3c2a455b0367773403c71a9cc/Kummel_2010.pdf
      Project name: not specified

    Experiment description
      Organism: Saccharomyces cerevisiae
      Strain: CEN.PK 113-7D, CEN.PK/JT4, FY4 
      Data type: metabolites at steady-state
      Data units: mM
      Execution date: not specified

    Experimental details
      Temperature (°C): 30
      pH: 5.0
      Carbon source: glucose
      Culture mode: batch
      Process condition: aerobic
      Dilution rate (h⁻¹): -
      Working volume: not specified L
      Biomass concentration (g/L): Calculated using an earlier determined OD-to-biomass DW correlation coefficient of 0.486 gDWL/OD for FY4 and 0.52 gDWL/OD for CEN.PK strains.
      Medium composition: Minimal defined medium with glucose as sole carbon source. Prepared from autoclaved salt and glucose solutions and sterile filtered solutions of vitamins and trace metals to reach concentrations as described in Verduyn et al. [1].
      General protocol information: Sampling Method: Intracellular metabolite concentrations of two biological and two technical replicates were determined from samples withdrawn from culture at an OD of approximately 1.5. Samples of 1–4mL were taken at each sampling time point.; Quenching: quenched in methanol at  -40 ºC. For the determination of the cAMP concentrations, a sample volume of 10mL was taken. After centrifuging for 3 min at 15 550 g in a rotor precooled to -9 ºC, the samples were frozen at -40 ºC.; Extraction list: hot ethanol; Analysis list: LC-MS, GC-TOF;
      Methods description: For quantification by GC-TOF, two sample aliquots were derivatized with either TMS-agent [N-methyl-N-(trimethylsilyl) trifluoroacetamide, Fluka] or TBDMS agent (N tertbutyldimethylsilyl-N-methyltrifluoroacetamide, Fluka). The samples were separated via GC on an HP5-MS column (Hewlett-Packard, length 30m ID 0.25 film 0.25 mm) and injected for MS analysis into a TOF spectrometer (Pegasus III, Leco). Detailed information on process parameters are described in Ewald et al. [2].                                                                                                                                                                                                                                                                                                                                          
 
-------------References------------
[1] Verduyn C, Postma E, ScheffersWA andVan Dijken JP (1992) Effect of benzoic acid on metabolic fluxes in yeasts: a continuousculture study on the regulation of respiration and alcoholic fermentation. Yeast 8: 501–517. http://doi.org/fgpqk3                                                                                                                                                                                    [2] Ewald JC, Heux S and Zamboni N (2009) High-throughput quantitative metabolomics: workflow for cultivation, quenching, and analysis of yeast in a multiwell format. Anal Chem 81: 3623–3629. http://doi.org/chgtcq
      Data file: http://kimosys.org/repository/93/download?parameter=1201; 
      Alternative formats: no files uploaded

    Submission and curation
      Entered by: Administrator KiMoSys
      Created: 2018-07-07 19:01:50 UTC
      Updated: 2020-04-24 16:10:35 UTC
      Version: 1
      Status: (reviewed) 2018-07-07 21:17:37 UTC
      Views: 290
      Downloads: 57