DataEntryID 79
    General information
      Manuscript title: Metabolic regulation analysis of wild-type and arcA mutant Escherichia coli under nitrate conditions using different levels of omics data.
      PubMed ID: http://www.ncbi.nlm.nih.gov/pubmed/22790675
      Journal: Molecular BioSystems
      Year: 2012
      Authors: Yoshihiro Toya, Kenji Nakahigashi, Masaru Tomita and Kazuyuki Shimizu
      Affiliations: Institute for Advanced Biosciences, Keio University, Tsuruoka 997-0017, Japan
      Keywords: Escherichia coli, intracelullar metabolites
      Full text article: no file uploaded
      Project name: not specified

    Experiment description
      Organism: Escherichia coli
      Strain: BW25113 and arcA mutant
      Data type: metabolites at steady-state
      Data units: (µM)
      Execution date: not specified

    Experimental details
      Temperature (°C): 37.0
      pH: 7.0
      Carbon source: glucose
      Culture mode: chemostat
      Process condition: aerobic and anaerobic
      Dilution rate (h⁻¹): 0.2
      Working volume: 1.0 L
      Biomass concentration (g/L): not specified
      Medium composition: Synthetic medium for batch and continuous: 48 mM Na2HPO4, 22 mM KH2PO4, 10 mM NaCl, 45 mM (NH4)2SO4, 4 g/L glucose was supplemented with 1 mM MgSO4, 1 mg mL 1 thiamine, 0.056 mg/L  CaCl2, 0.08 mg/L FeCl3, 0.01 mg/L MnCl2.4H2O, 0.017 mg/L ZnCl2, 0.0043 mg/L CuCl2.2H2O, 0.006 mg/L CoCl2.2H2O, and 0.06 mg/L Na2MoO4.2H2O. In addition, 40 mM of potassium nitrate was added to the medium for the cultures under nitrate conditions.
      General protocol information: Sampling Method: 5 mg of cells were passed through a 0.45 mm pore filter (Millipore) filled with saline.; Quenching: filter washed twice with 10 mL of Milli-Q water and  quickly plunged into 5 mL of methanol containing 2 mM CSA and methionine sulfone as internal standards within 1 min.; Extraction list: chloroform, sonication; Analysis list: CE-TOF-MS;
      Methods description: Capillary electrophoresis time-of-flight mass spectrometry (CE-TOFMS) measurements were performed on an Agilent CE capillary electrophoresis system (Agilent Technologies) and an Agilent G3250AA LC/MSD TOF system (Agilent Technologies). The raw CE-TOFMS data were analyzed using MasterHands software.
      Data file: http://kimosys.org/repository/79/download?parameter=1182; 
      Alternative formats: http://kimosys.org/repository/79/attached_files/143/download; http://kimosys.org/repository/79/attached_files/142/download; http://kimosys.org/repository/79/attached_files/141/download; http://kimosys.org/repository/79/attached_files/140/download; http://kimosys.org/repository/79/attached_files/111/download; http://kimosys.org/repository/79/attached_files/110/download; http://kimosys.org/repository/79/attached_files/109/download; http://kimosys.org/repository/79/attached_files/108/download; 

    Submission and curation
      Entered by: Administrator KiMoSys
      Created: 2013-07-08 15:05:36 UTC
      Updated: 2020-04-24 16:10:34 UTC
      Version: 0
      Status: (reviewed) 2013-12-06 17:17:38 UTC
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      Downloads: 50