DataEntryID 77 General information Manuscript title: Integrating flux balance analysis into kinetic models to decipher the dynamic metabolism of Shewanella oneidensis. PubMed ID: http://www.ncbi.nlm.nih.gov/pubmed/22319437 Journal: PLOS Computational Biology Year: 2012 Authors: Xueyang Feng, You Xu, Yixin Chen, Yinjie J. Tang Affiliations: Department of Energy, Environmental and Chemical Engineering, Washington University, St. Louis, Missouri, United States of America Keywords: Shewanella oneidensis, dynamic metabolism, extracellular Full text article: https://kimosys.org/rails/active_storage/blobs/eyJfcmFpbHMiOnsibWVzc2FnZSI6IkJBaHBBcHNFIiwiZXhwIjpudWxsLCJwdXIiOiJibG9iX2lkIn19--11fed0a65063d72adb969872230557807060797e/Feng_2012.pdf Project name: not specified Experiment description Organism: Shewanella oneidensis Strain: MR-1 (ATCC 70050) Data type: time-series data of metabolites Data units: (g/L) and (mM) Execution date: not specified Experimental details Temperature (°C): 30.0 pH: not specified Carbon source: lactate Culture mode: batch Process condition: aerobic Dilution rate (h⁻¹): — Working volume: 0.1 L Biomass concentration (g/L): ≈ 0.3 - 0.5 Medium composition: defined medium General protocol information: Sampling Method: manually at different time; Quenching: —; Extraction list: not used; Analysis list: enzymatic; Methods description: The growth curve was monitored by dried biomass weight. The concentrations of lactate and acetate in the medium were measured using enzyme kits (r-Biopharm, Darmstadt, Germany). The concentration of pyruvate in the medium was measured with the enzyme assay developed by Marbach and Weli [1]. --------------------References------------------- [1] Marbach EP, Weli MH (1967). Rapid enzymatic measurement of blood lactate and pyruvate. Use and significance of metaphosphoric acid as a common precipitant. Clin Chem 13: 314–325. Data file: http://kimosys.org/repository/77/download?parameter=1178; Alternative formats: http://kimosys.org/repository/77/attached_files/102/download; Submission and curation Entered by: Administrator KiMoSys Created: 2013-07-03 16:40:55 UTC Updated: 2020-04-24 16:10:34 UTC Version: 0 Status: (reviewed) 2013-12-06 17:17:38 UTC Views: 270 Downloads: 127