DataEntryID 77
    General information
      Manuscript title: Integrating flux balance analysis into kinetic models to decipher the dynamic metabolism of Shewanella oneidensis.
      PubMed ID: http://www.ncbi.nlm.nih.gov/pubmed/22319437
      Journal: PLOS Computational Biology
      Year: 2012
      Authors: Xueyang Feng, You Xu, Yixin Chen, Yinjie J. Tang
      Affiliations: Department of Energy, Environmental and Chemical Engineering, Washington University, St. Louis, Missouri, United States of America
      Keywords: Shewanella oneidensis, dynamic metabolism, extracellular
      Full text article: https://kimosys.org/rails/active_storage/blobs/eyJfcmFpbHMiOnsibWVzc2FnZSI6IkJBaHBBcHNFIiwiZXhwIjpudWxsLCJwdXIiOiJibG9iX2lkIn19--11fed0a65063d72adb969872230557807060797e/Feng_2012.pdf
      Project name: not specified

    Experiment description
      Organism: Shewanella oneidensis
      Strain: MR-1 (ATCC 70050)
      Data type: time-series data of metabolites
      Data units: (g/L) and (mM)
      Execution date: not specified

    Experimental details
      Temperature (°C): 30.0
      pH: not specified
      Carbon source: lactate
      Culture mode: batch
      Process condition: aerobic
      Dilution rate (h⁻¹): —
      Working volume: 0.1 L
      Biomass concentration (g/L): ≈ 0.3 - 0.5
      Medium composition: defined medium
      General protocol information: Sampling Method: manually at different time; Quenching: —; Extraction list: not used; Analysis list: enzymatic;
      Methods description: The growth curve was monitored by dried biomass weight. The concentrations of lactate and acetate in the medium were measured using enzyme kits (r-Biopharm, Darmstadt, Germany). The concentration of pyruvate in the medium was measured with the enzyme assay developed by Marbach and Weli [1].

--------------------References-------------------
[1] Marbach EP, Weli MH (1967). Rapid enzymatic measurement of blood lactate and pyruvate. Use and significance of metaphosphoric acid as a common precipitant. Clin Chem 13: 314–325.
      Data file: http://kimosys.org/repository/77/download?parameter=1178; 
      Alternative formats: http://kimosys.org/repository/77/attached_files/102/download; 

    Submission and curation
      Entered by: Administrator KiMoSys
      Created: 2013-07-03 16:40:55 UTC
      Updated: 2020-04-24 16:10:34 UTC
      Version: 0
      Status: (reviewed) 2013-12-06 17:17:38 UTC
      Views: 223
      Downloads: 112