DataEntryID 73
    General information
      Manuscript title: Overexpression of isocitrate lyase-glyoxylate bypass influence on metabolism in Aspergillus niger.
      PubMed ID: http://www.ncbi.nlm.nih.gov/pubmed/19271267
      Journal: Metabolic Engineering
      Year: 2009
      Authors: S. Meijer, J Otero, R. Olivares, M.R. Andersen, L. Olsson, J. Nielsen
      Affiliations: Department for Systems Biology, Center for Microbial Biotechnology, Building 223, Technical University of Denmark, Lyngby, Denmark.
      Keywords: Aspergillus niger, isocitrate lyase over-expression, glyoxylate bypass
      Full text article: https://kimosys.org/rails/active_storage/blobs/eyJfcmFpbHMiOnsibWVzc2FnZSI6IkJBaHBBcE1FIiwiZXhwIjpudWxsLCJwdXIiOiJibG9iX2lkIn19--de385283cd87256bce2e67238dc897b73186ba07/Meijer_2009.pdf
      Project name: not specified

    Experiment description
      Organism: Aspergillus niger
      Strain: N402 (W.T.) and icl overexpression
      Data type: flux measurements
      Data units: % substrate uptake
      Execution date: not specified

    Experimental details
      Temperature (°C): 30.0
      pH: 2.5
      Carbon source: glucose
      Culture mode: batch
      Process condition: aerobic
      Dilution rate (h⁻¹): not specified
      Working volume: 2.0 L
      Biomass concentration (g/L): 2.0-4.0
      Medium composition: Defined medium: 5 g/L of 1-13C glucose, 10 g/L NaNO3, 3 g/L KH2PO4, 2 g/L MgSO4.7H2O, 2 g/L NaCl, 0.2 g/L CaCl2.2H2O, 0.5 ml/L antifoam and 1 ml/L trace element solution 2. The trace element solution 2 contained 14.3 g/L ZnSO4.7H2O, 2.5 g/L CuSO4.5H2O, 0.5 g/L NiCl2.6H2O, 7 g/L MnCl2.2H2O and 13.8 g/L FeSO4.7H2O.
      General protocol information: Type analysis list: 13C constrained MFA; Platform list: GC-MS;
      Methods description: GC–MS analysis - GC–MS analysis was performed on an Agilent (PaloAlto,CA, USA) HP 6890 gas chromatograph coupled to a HP5973 quadrupole mass selective detector in positive electron impact ionization (EI+) operated at an electron energy of 70 eV. The GC was equipped with a 4.0mm i.d. Siltek gooseneck splitless deactivated liner (Restek, Bellefonte, PA, USA), and a Supelco (Bellefonte,PA,USA) SLB-5 MS column, 15m, 0.25mm i.d., 0.25 µm film. Helium of a purity of 99.999% was used as carrier gas at a constant linear gas velocity of 35 cm/s. Transfer line temperature was 280 ºC, quadrupole temperature 150 ºC and MS source 200 ºC. The GC–MS system was controlled from Agilent MSD Chemstation v. D.01.02.16. For both methods (ECF and DMF/DMA) 1 mL sample was injected in the splitless (30s, split 1:20) mode at 200 ºC using a hot needle.The MS scanrange was m/z 40–400 with 2.88 s/scan. To avoid sample carry-over the syringe was cleaned using five times 5mL acetone, then five times5 mL dichloromethane and finally 3 times rinsing with 3 µL sample.
Metabolic network analysis - The fluxes were determined using an in-house program code in Matlab V7.0.4 , which implements the metabolic network analysis framework from [1].

--------------References------------------
[1] Wiechert, W.,2001. C13 metabolic flux analysis. Metab. Eng. 3, 195–206. http://doi.org/dr89ms 
      Data file: http://kimosys.org/repository/73/download?parameter=1170; 
      Alternative formats: http://kimosys.org/repository/73/attached_files/120/download; http://kimosys.org/repository/73/attached_files/119/download; http://kimosys.org/repository/73/attached_files/92/download; http://kimosys.org/repository/73/attached_files/91/download; 

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      Entered by: Administrator KiMoSys
      Created: 2013-06-23 12:31:56 UTC
      Updated: 2020-04-24 16:10:34 UTC
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